SEC61A1/SEC61A2 Recombinant Monoclonal Antibody

SEC61A1 and SEC61A2 encode the alpha subunits of the Sec61 translocon complex, a highly conserved heterotrimeric channel embedded in the endoplasmic reticulum membrane. This complex serves as the primary gateway for co-translational and post-translational protein translocation into the ER, making it essential for the biosynthesis of secretory and membrane proteins. Given its central role in proteostasis, the Sec61 complex has emerged as a focus in research spanning ER stress responses, unfolded protein response signaling, and therapeutic targeting in oncology.

This recombinant monoclonal antibody, derived from rabbit host and produced using defined genetic sequences, offers the consistency and reproducibility that demanding experimental workflows require. Unlike traditional hybridoma-derived antibodies, recombinant production ensures lot-to-lot uniformity, allowing you to confidently compare results across extended studies without concerns about antibody variability affecting your data.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis confirms specific detection at the predicted 52 kDa molecular weight across diverse human cell lines including U-251MG, SH-SY5Y, A431, HepG2, HeLa, and A549, with cross-species reactivity confirmed in mouse and rat liver tissue lysates. Immunohistochemistry staining in paraffin-embedded human colorectal cancer tissue reveals clear target localization, while immunofluorescence in HeLa cells and flow cytometry analysis in HepG2 cells further expand your application options for subcellular localization and quantitative single-cell studies.

Whether investigating ER translocation mechanisms, characterizing secretory pathway dysfunction in disease models, or exploring Sec61 as a therapeutic vulnerability, this antibody provides reliable detection across human, mouse, and rat samples to support your translational research objectives.