SENP2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA950918A0HU
Size	US$210
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Image	IHC image of CSB-RA950918A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA950918A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA950918A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA950918A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9HC62

Target Names

SENP2

Alternative Names

Axam2 antibody; DKFZp762A2316 antibody; KIAA1331 antibody; Senp2 antibody; SENP2_HUMAN antibody; Sentrin specific protease 2 antibody; Sentrin-specific protease 2 antibody; Sentrin/SUMO specific protease SENP2 antibody; Sentrin/SUMO-specific protease SENP2 antibody; SMT3 specific isopeptidase 2 antibody; SMT3-specific isopeptidase 2 antibody; Smt3ip2 antibody; SUMO1/sentrin/SMT3 specific peptidase 2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human SENP2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15B5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SENP2 (Sentrin-specific protease 2) plays a central role in the SUMOylation pathway, functioning as a cysteine protease that cleaves SUMO precursors and removes SUMO modifications from target proteins. This dynamic regulation of SUMOylation influences diverse cellular processes including transcriptional regulation, nuclear transport, and cell cycle progression, making SENP2 a compelling target for researchers investigating post-translational modification networks and their implications in disease states, particularly cancer biology.

This recombinant monoclonal antibody (clone 15B5) offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for human SENP2, while affinity chromatography purification delivers a reagent ready for sensitive detection applications.

Validation studies demonstrate robust performance across multiple platforms, providing flexibility for researchers approaching SENP2 biology from different experimental angles. Immunohistochemistry staining has been successfully performed in paraffin-embedded human skin tissue and human liver cancer sections using citrate buffer antigen retrieval, revealing clear target localization with DAB visualization. Immunofluorescence analysis in HepG2 cells shows distinct staining patterns when counter-stained with DAPI, enabling subcellular localization studies. Flow cytometry validation in HeLa cells confirms the antibody's utility for quantitative single-cell analysis, with clear separation from isotype control.

Whether investigating SENP2's role in cancer progression, exploring SUMOylation dynamics in cellular stress responses, or characterizing expression patterns across tissue types, this antibody provides a reliable tool for advancing cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Deconjugates SUMO2 from MTA1. Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB.

Gene References into Functions

research achievements of SENP2 are reviewed in order to understand its related functions and the underlying molecular mechanisms and provide a clue for future research on SENP2 PMID: 29908207
Quantitative high-throughput screening identifies cytoprotective molecules that enhance SUMO conjugation via the inhibition of SUMO-specific protease (SENP)2. PMID: 29146736
Data demonstrate that downregulation of SENP2 is correlated with poor prognosis in bladder cancer; SENP2 inhibits TGF-beta signaling and TGF-beta-induced EMT of bladder cancer cell; and that its overexpression contributes to suppress bladder cancer cell invasion and metastasis through deSUMOylation of TGF-betaRI. PMID: 28574613
The variability of the SENP1 and SENP2 genes may play a role in breast cancer occurrence. PMID: 27178176
SENP2 inhibits MMP13 expression in BC cells through de-SUMOylation of TBL1/TBLR1, which inhibits nuclear translocation of beta-catenin. PMID: 26369384
miR-181b targets SENP2 and positively regulated NF-kappaB activity. NF-kappaB activation by DNA damage in GBM cells confers resistance to radiation-induced death. PMID: 25633526
data identify SENP2 as an important regulator of fatty acid metabolism in skeletal muscle PMID: 25784542
SENP2 regulates the transcriptional function of MEF2A via direct de-SUMOylation. PMID: 25483061
p90RSK-mediated SENP2-T368 phosphorylation is a master switch in disturbed-flow-induced signaling. PMID: 25689261
ESR1 repression by SENP2 is independent of its SUMO protease activity. PMID: 24422630
Many nucleoporins are mislocalized and, in some cases, reduced in level when SENP1 and SENP2 are codepleted. PMID: 24196834
Chromosome segregation depends on precise spatial and temporal control of sumoylation in mitosis; SENP1 and SENP2 are important mediators of this control. PMID: 24048451
The SENP1 levels are influenced by the presence of Nup153, whereas SENP2 is not sensitive to changes in Nup153 abundance. PMID: 22688647
SENP2 inhibited bladder cancer cells migration and invasion by regulating the expression of MMP13. PMID: 24008762
SENP2 plays an important role in determining the dynamics and functional outcome of MEF2A SUMOylation and transcriptional activation. PMID: 23224591
SENP2 represses glycolysis and shifts glucose metabolic strategy PMID: 23691130
SENP2 regulates hepatocellular carcinoma cell growth by modulating the stability of beta-catenin. PMID: 23098437
cardiac overexpression of SENP2 in the mice with Nkx2.5 haploinsufficiency promoted embryonic lethality and severity of congenital heart diseases (CHDs). PMID: 22155005
Data suggest that SENP2 regulates antiviral innate immunity by deSUMOylating IRF3 and conditioning it for ubiquitination and degradation, and provide an example of cross-talk between the ubiquitin and SUMO pathways in innate immunity. PMID: 22028379
SENP2 null cells display biphasic NEMO SUMOylation and activation of IKK and NF-kappaB PMID: 21777808
a mechanism underlying the SENP2-mediated regulation of Mdm2 that is critical for genome integrity in p53-dependent stress responses. PMID: 21183956
Results demonstrate that SENP1 is the most efficient SUMO protease acting on Elk-1, and that SENP3 has little effect on Elk-1. SENP2 has an intermediate effect, but its ability to activate Elk-1 is independent from its SUMO-deconjugating activity. PMID: 20337593
association with the pore plays an important negative role in the regulation of SENP2 PMID: 11896061
Restricting SENP2 in the nucleus by mutations in the NES impairs its polyubiquitination, whereas a cytoplasm-localized SENP2 made by introducing mutations in the NLS can be efficiently polyubiquitinated. PMID: 16738331
Mutational analysis and biochemistry provide a mechanism for SENP2 substrate preferences that explains why SENP2 catalyzes SUMO deconjugation more efficiently than processing PMID: 17099700

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Subcellular Location

Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Cytoplasm.

Protein Families

Peptidase C48 family

Database Links

HGNC: 23116

OMIM: 608261

KEGG: hsa:59343

STRING: 9606.ENSP00000296257

UniGene: Hs.401388

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Wnt signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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