Product Details

Uniprot No.

Target Names

SEPTIN2

Alternative Names

SEPTIN2 antibody; DIFF6 antibody; KIAA0158 antibody; NEDD5 antibody; SEPT2 antibody; Septin-2 antibody; Neural precursor cell expressed developmentally down-regulated protein 5 antibody; NEDD-5 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human SEPTIN2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13F7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SEPTIN2 belongs to the septin family of GTP-binding proteins that play essential roles in cytokinesis, membrane dynamics, and cytoskeletal organization. As a core component of septin filaments, SEPTIN2 contributes to the formation of diffusion barriers at the cell membrane and participates in coordinating cell division. Its involvement in these fundamental cellular processes makes it a valuable target for researchers investigating cell cycle regulation, membrane compartmentalization, and cytoskeletal remodeling in both normal physiology and disease states.

This recombinant monoclonal antibody, generated from clone 13F7, offers the consistency and reliability that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, researchers benefit from lot-to-lot reproducibility that supports longitudinal studies and ensures comparable results across experiments. The rabbit IgG format and affinity-chromatography purification further contribute to specificity and low background in detection applications.

Validation studies demonstrate robust performance in Western blot analysis, where the antibody detects SEPTIN2 at the expected 41 kDa molecular weight across multiple human cell lines including PC-3, HeLa, A549, A-431, and HEK293. This concordance between predicted and observed band sizes confirms target specificity. For flow cytometry applications, the antibody has been validated using 786-O renal carcinoma cells with intracellular staining protocols, showing clear separation from isotype control and enabling quantitative analysis of SEPTIN2 expression at the single-cell level.

This antibody serves researchers exploring cytokinesis mechanisms, septin biology, and cytoskeletal dynamics, providing a dependable tool for both qualitative and quantitative assessment of SEPTIN2 in human cell models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Gene References into Functions

Results demonstrate that in stored platelets, septine-2 and septin-6 mRNAs have miR- 223 target sites, septin-2 and septin-6 are in complex with Ago-2. The results demonstrate that like in nucleated cells, enucleated platelets also have microRNA-based mechanisms for the regulation of their septins. PMID: 29943706
Study discloses both SEPT2 and SEPT7 are essential for breast cancer cell migration and invasion by controlling MEK/ERK MAPKs activation. PMID: 27557506
Depletion of septin 2 reduces Drp1 recruitment to mitochondria and results in hyperfused mitochondria and delayed FCCP-induced fission. PMID: 27215606
septins protect ErbB2 from ubiquitylation, endocytosis and lysosomal degradation. Septin oligomerization regulates persistent expression of ErbB2/HER2 in gastric cancer cells. PMID: 27048593
The results showed that SEPT2 has an oncogenic function through accelerated cell proliferation and invasion in biliary tract cancers, and is negatively regulated by miR-140-5p. PMID: 27155525
From the nucleotide-free structure some interesting conclusions about the nucleotide binding properties of Cdc11 can be drawn; especially when aligning the structure with the structure of GDP bound Sept2 PMID: 26780475
These results suggest that SEPTIN2-mediated cytoskeletal rearrangement and STATHMIN-mediated differentiation may contribute to changes in cell morphology and differentiation of H/RS cells with CD99 upregulation in Hodgkin lymphoma. PMID: 26000982
Data indicate that forchlorfenuron (FCF) exhibits differential binding preference for septins SEPT2 and SEPT3. PMID: 24787956
Authors report here that the septins SEPT2, -9, -11, and probably -7 form fibrillar structures around the chlamydial inclusion. PMID: 25293760
Suggest PPAR-gamma activation down-regulates hepatocellular carcinoma cell SEPT2 levels to prevent tumor growth. PMID: 25592041
SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9. PMID: 23572511
The results shown in study support the hypothesis that single Septin 2, when present in excess or with unbalanced stoichiometries, may be unstable and assemble into amyloid-like structures. PMID: 21967827
Data illustrated roles of SEPT9 that might contribute to hetero-trimeric septin complex formation. SEPT9 can substitute for septins of the SEPT2 group and partially for SEPT7. PMID: 21767235
The SEPT2 provides the directional guidance cues necessary for polarizing the epithelial microtubule network. PMID: 21788367
septin-2 plays a fundamental role in regulating barrier function by altering cortical actin expression. PMID: 20870893
the role of SEPT2 and SEPT11 in the InlB-Met interactions PMID: 21504731
Data show that Septins of the SEPT6 group preferentially interacted with septins of the SEPT2 group, SEPT3 group and SEPT7 group. PMID: 21082023
Nedd5 is involved in the process of cytokinesis in human brain tumours. PMID: 12125979
septins may form a mitotic scaffold for CENP-E and other effectors to coordinate cytokinesis with chromosome congression and segregation PMID: 15774761
Nedd5 C-terminal is a novel autoantigen in systemic lupus erythematosus (SLE) patients and may be a valuable tool for diagnosing neuropsychiatric SLE. PMID: 15987492
protein encoded by SEPT2 is highly homologous to septins 1, 4 and 5 and is involved in the coordination of several key steps of mitosis PMID: 16682951
septin 2, 6, and 7 complexes make up polymerized filaments PMID: 16914550
crystal structures of the human SEPT2 G domain and the heterotrimeric human SEPT2-SEPT6-SEPT7 complex PMID: 17637674
Study shows that in epithelial cells septin 2 fibers colocalize with a subset of microtubule tracks composed of polyglutamylated tubulin. PMID: 18209106
A novel MLL-SEPT2 fusion variant in therapy-related myelodysplastic syndrome is reported. PMID: 18656699
confirmed that the phosphorylation of SEPT2 on Ser218 by CK2 was crucial to the proliferation of HCC. These results suggest that SEPT2 might be a promising target for liver cancer therapy PMID: 19165576
SEPT2 is essential for the InlB-mediated entry of Listeria, but SEPT11 is not, which distinguishes the roles of different mammalian septins PMID: 19234302

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Midbody. Cytoplasm, cell cortex. Cell projection, cilium membrane. Cell projection, cilium, flagellum.

Protein Families

TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family

Tissue Specificity

Widely expressed. Up-regulated in liver cancer.

Database Links

HGNC: 7729

OMIM: 601506

KEGG: hsa:4735

STRING: 9606.ENSP00000353157

UniGene: Hs.721234

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Code	CSB-RA982588A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: PC-3 whole cell lysate(30µg), HeLa whole cell lysate(30µg), A549 whole cell lysate, A-431 whole cell lysate(30µg), HEK293 whole cell lysate(30µg) All lanes: SEPTIN2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 2min Overlay Peak curve showing 786-O cells stained with CSB-RA982588A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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SEPTIN2 Recombinant Monoclonal Antibody

Product Details

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Target Background