Product Details

Uniprot No.

Target Names

Alternative Names

EAA3_HUMAN antibody; EAAC 1 antibody; EAAC1 antibody; EAAT 3 antibody; Excitatory amino acid carrier 1 antibody; Excitatory amino acid carrier1 antibody; Excitatory amino acid transporter 3 antibody; Excitatory amino acid transporter3 antibody; Excitatory amino-acid carrier 1 antibody; GLUTAMATE TRANSPORTER; HIGH-AFFINITY antibody; MEAAC 1 antibody; MEAAC1 antibody; Neuronal and epithelial glutamate transporter antibody; REAAC 1 antibody; REAAC1 antibody; Slc1 a1 antibody; Slc1a 1 antibody; SLC1A1 antibody; Sodium dependent glutamate/aspartate transporter 3 antibody; Sodium-dependent glutamate/aspartate transporter 3 antibody; Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter; system Xag); member 1 antibody; SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER); MEMBER 1 antibody; Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter; system Xag); member 1 antibody; Solute carrier family 1 member 1 antibody; Solute carrier family 1; member 1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human SLC1A1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4A11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SLC1A1, also known as EAAT3 or EAAC1, serves as the primary neuronal glutamate transporter responsible for maintaining precise extracellular glutamate concentrations in the central nervous system. This high-affinity sodium-dependent transporter plays a critical role in preventing excitotoxicity by rapidly clearing glutamate from synaptic clefts, making it an essential target for researchers investigating neurodegenerative diseases, epilepsy, and psychiatric disorders including obsessive-compulsive disorder, where genetic variants in SLC1A1 have been implicated.

This recombinant monoclonal antibody, generated against a synthesized peptide from human SLC1A1, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host, you can expect uniform performance across lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The affinity-chromatography purification ensures high specificity for your target.

Validation in immunohistochemistry demonstrates reliable detection in formalin-fixed, paraffin-embedded human tissue sections. Testing in human liver cancer tissue using a Leica Bond system with citrate buffer antigen retrieval at pH 6.0 confirmed specific staining at 1:100 dilution, with recommended working concentrations ranging from 1:50 to 1:200 depending on your sample and detection system. The antibody has also been validated for ELISA applications, providing flexibility across different experimental platforms.

Whether you are mapping SLC1A1 expression patterns in human tissue specimens, investigating transporter dysfunction in neurological disease models, or characterizing glutamate signaling pathways, this antibody delivers the specificity and batch consistency essential for generating publication-quality data in neuroscience research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport. Negatively regulated by ARL6IP5.

Gene References into Functions

mir-26a and SLC1A1 may play a role in the Multiple Sclerosis pathogenesis, and may be potential targets for the development of new biomarkers and/or therapeutic tools. PMID: 28962897
SLC1A1 and DPF3 were strongly associated with idiopathic male infertility and were significantly correlated with semen quality alterations PMID: 27232852
results suggest that six common SNPs of SLC1A1 may not contribute to the development of obsessive compulsive disorder, but may contribute to certain aspect of trait empathy such as personal distress PMID: 29304071
observations of EAAT3 regulation in patients is giving us insight into the endogenous function of this transporter, as well as the consequences of altered function--{REVIEW} PMID: 27233497
Our study showed a lack of association between SLC1A1 variants and normal tension glaucoma in Japanese patients, suggesting that the SLC1A1 gene does not play a critical role in the development of the disorder in this patient population. PMID: 26771863
Numb is a pivotal adaptor protein that mediates the subcellular localization of EAAT3 through binding the YxNxxF (where x stands for any amino acid) motif. PMID: 27358480
observations provide insights into the molecular basis of hot water epilepsy and show the role of SLC1A1 variants in this intriguing neurobehavioral disorder PMID: 28324217
This study demonstrated that Variations within SLC1A1 are associated with risk of epileptogenesis following posttraumatic brain injury. PMID: 27153812
Lithium-sensitive GSK3ss is a powerful regulator of excitatory amino acid transporters EAAT3 and EAAT4. PMID: 27978527
Data suggest that EAAC1 transporters present on NST dendrites may play a minor role if any in glutamate clearance. PMID: 25515313
SPAK and OSR1 are negative regulators of EAAT3 activity PMID: 26112741
Excitatory amino acid carrier 1 (EAAC1) plays a pivotal role in neuronal glutathione synthesis to maintain cellular redox homeostasis. PMID: 26007177
Transport of either L-glutamate or L-selenocysteine by EAAT3 decreased intracellular pH, whereas transport of cysteine resulted in cytoplasmic alkalinization. PMID: 25275463
Rs301430 is a T/C functional polymorphism that influences ago of onset in obsessive-compulsive disorder. PMID: 24768158
variability within the SLC1A1 gene impacts both the presence and severity of posttraumatic stress disorder among a sample of combat-exposed veterans. PMID: 25445080
Our data suggest that SLC1A1 is unlikely to be a major susceptibility gene for schizophrenia in Han Chinese PMID: 23931931
Results indentify plausible candidates include non-recurrent deletions at the glutamate transporter gene SLC1A1, a CNV locus recently suggested to be involved in schizophrenia through linkage analysis, and duplications at 1p36.33 and CGNL1. PMID: 24163246
internalization of EAAT3 triggered by amphetamine increases glutamatergic signaling and thus contributes to the effects of amphetamine on neurotransmission. PMID: 25033183
EAAT3/EAAC1 expression is altered in pathological conditions, such as hypoxia/ischemia, multiple sclerosis, schizophrenia, and epilepsy. (Review) PMID: 24162932
SLC1A1, GRIN2B, and interactions between the two may potentially confer a susceptibility to obsessive-compulsive symptoms in schizophrenia patients receiving clozapine. PMID: 23660601
This study demonistrated that SLC!A1 no association with obsessive-compulsive disorder in Han Chinese population. PMID: 23411042
A single-marker association study of several single-nucleotide polymorphisms in the SLC1A1 genomic region was performed. PMID: 22776887
Individuals with schizophrenia carrying at least one C allele of rs35753505 (SNP8NRG221533) show decreased expression of SLC1A6 in the molecular layer of both cerebellar hemispheres, compared to individuals homozygous for the T allele. PMID: 22424243
This study demonisrrated that glutamate aspartate transporter (GLAST) within the human cochlea in patient with Meniere's disease. PMID: 23850643
P259R alters EAAT3 transport functions by decelerating conformational changes associated with sodium binding. PMID: 24214974
isoforms negatively modulate glutamate uptake and are differentially expressed in subjects with obsessive-compulsive disorder PMID: 23695234
The study provided suggestive evidence that the SLC1A1 rs10491734 was significantly associated with early-onset OCD in the Han Chinese population. PMID: 23564280
revealed weak association between OCD and one of nine tested SLC1A1 polymorphisms PMID: 23606572
A deletion at the SLC1A1 glutamate transporter gene originally identified as a copy number variant exclusively carried by members of a 5-generation Palauan family, was investigated. PMID: 23341099
The function of the one SLC1A1 missense variant reported previously in obsessive-compulsive disorder, Thr164Ala, was characterized and found that the Ala164 allele leads to decreased Vmax and Km. PMID: 22617815
Three single nucleotide polymorphisms in SLC1A1 (rs2228622, rs3780412 and rs3780413), which had been associated with second-generation antipsychotic agents-induced obsessive-compulsive symptoms, were investigated. PMID: 22531293
rapid extracellular K(+) application to EAAC1 under single turnover conditions (K(+) inside) resulted in outward transient current. We propose a charge compensation mechanism, in which the C-terminal transport domain bears overall negative charge of -1.23 PMID: 22707712
interaction of NCX1 and EAAC1 transporters leads to glutamate-enhanced ATP production in brain mitochondria PMID: 22479505
Neutralizing aspartate 83 modifies substrate translocation of excitatory amino acid transporter 3 (EAAT3) glutamate transporters. PMID: 22532568
mTOR is a novel powerful regulator of EAAT3 and may thus contribute to protection against neuroexcitotoxicity. PMID: 22483750
These findings suggest the involvement of SLC1A1 in the pathogenesis of schizophrenia. PMID: 22095641
This study provides suggestive evidence that DLGAP3 and its interactive effect with SLC1A1 might be involved in susceptibility to developing OC symptoms in schizophrenia patients receiving AAP treatment. PMID: 21990008
genotyped an additional 111 SNPs in or near SLC1A1, covering from 9 kb upstream to 84 kb downstream of the gene at average spacing of 1.7 kb per SNP, and conducted family-based association analyses in 1,576 participants in 377 families PMID: 21445956
SLC1A1 mutations leading to substitution of arginine to tryptophan at position 445 (R445W) and deletion of isoleucine at position 395 (I395del) cause dicarboxylic aminoaciduria, an autosomal recessive disorder of urinary glutamate and aspartate transport PMID: 21123949
SLC1A1 alleles may have a role in obsessive-compulsive disorder PMID: 19569082
Cysteine uptake by EAAC1 is important for zinc homeostasis and neuronal antioxidant function following transient cerebral ischemia. PMID: 21084597
association of SNP rs301430 with severity of repetitive behaviors and anxiety in autism spectrum disorder PMID: 20155310
suggests that T370S also alters the cation selectivity of this cation-binding site, as expected if T370 forms part of a cation-binding site in EAAT3. PMID: 20634426
the side chain of EAAC1 Met-367 fulfills a steric role in the positioning of the substrate in the binding pocket in a step subsequent to its initial binding PMID: 20424168
analysis of cation binding to the glutamate transporter EAAC1 probed with mutation of the conserved amino acid residue Thr10 PMID: 20378543
PIP5K2A is a novel signaling element in the regulation of EAAT3 activity PMID: 19644675
Dynamic equilibrium between coupled and uncoupled modes of a neuronal glutamate transporter PMID: 11823462
data show specific localization of glutamate transporters EAAT1, EAAT2 and EAAT3 in the human placenta during development PMID: 15135231
EAAT3 has a big extracellular vestibule, but does not undergo a large-scale motion to another state. Short channels connect the intracellular solution to the vestibule. Small conformational changes & glutamate uptake, occur independently in each subunit. PMID: 15716409
enhanced expression of glutamate transporters in multiple sclerosis constitutes a regulatory response of glial cells to toxic levels of glutamate in the CNS during inflammation and neurodegeneration PMID: 16061389

Hide All

Involvement in disease

Dicarboxylic aminoaciduria (DCBXA); Schizophrenia 18 (SCZD18)

Subcellular Location

Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. Early endosome membrane. Late endosome membrane. Recycling endosome membrane.

Protein Families

Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A1 subfamily

Tissue Specificity

Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).

Database Links

HGNC: 10939

OMIM: 133550

KEGG: hsa:6505

STRING: 9606.ENSP00000262352

UniGene: Hs.444915

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Code	CSB-RA548433A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA548433A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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SLC1A1 Recombinant Monoclonal Antibody

Product Details

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Target Background