SLC2A3 Recombinant Monoclonal Antibody

Code CSB-RA162667A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: Jurkat whole cell lysate
    All lanes: SLC2A3 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 54 kDa
    Observed band size: 55-70 kDa
  • IHC image of CSB-RA162667A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA162667A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA162667A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Overlay Peak curve showing MCF7 cells stained with CSB-RA162667A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Target Names
SLC2A3
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 3 (Glucose transporter type 3, brain) (GLUT-3), SLC2A3, GLUT3
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human SLC2A3
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
14B1
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

CUSABIO meticulously generated the SLC2A3 recombinant monoclonal antibody through a systematic approach. Initially, B cells were isolated from the spleen of an animal immunized with the a synthesized peptide derived from human SLC2A3. Subsequently, RNA was extracted from the B cells and reverse-transcribed into cDNA. The gene encoding the SLC2A3 antibody was then amplified using a degenerate primer and inserted into a vector. Through transfection, the recombinant vector was introduced into host cells, enabling efficient expression of the SLC2A3 recombinant monoclonal antibodies. These antibodies were subsequently harvested from the cell culture supernatant and subjected to purification using affinity chromatography. Rigorous validation, including ELISA, WB, IHC, and FC testings, was performed to confirm the specific reactivity of the SLC2A3 recombinant monoclonal antibody with human SLC2A3 protein, ensuring its reliability and suitability for various applications.

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Target Background

Function
Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport.
Gene References into Functions
  1. means of immunolabeled cells for GLUT-3 varied approximately from 19% to 73% PMID: 30049187
  2. PPAR-gamma and Akt regulate GLUT1 and GLUT3 surface localization during Mycobacterium tuberculosis infection PMID: 28852964
  3. miR-29c was down-regulated in Prostate cancer samples. SLC2A3, a regulator of glycolysis, was validated as a direct target of miR-29c. Moreover, functional studies showed miR-29c could inhibit cell growth, induce apoptosis and deceased the rate of glucose metabolism. PMID: 29715514
  4. Both common and rare SLC2A3 variation impacting regulation of neuronal glucose utilization and energy homeostasis may result in neurocognitive deficits known to contribute to ADHD risk. PMID: 28224622
  5. N-methyl-D aspartate (NMDA) receptor activity increases GLUT3 expression. PMID: 28951307
  6. identify a subset of tumors within the "proneural" and "classical" subtypes that are addicted to aberrant signaling from integrin alphavbeta3, which activates a PAK4-YAP/TAZ signaling axis to enhance Glut3 expression PMID: 29198914
  7. GLUT3-mediated glucose utilization and glycogenolysis in platelets promotes alpha-granule release, platelet activation, and postactivation functions. PMID: 28663252
  8. effects of physiologically relevant phospholipids on glucose transport in liposomes containing purified GLUT4 and GLUT3. The anionic phospholipids, phosphatidic acid, phosphatidylserine, phosphatidylglycerol, and phosphatidylinositol, were found to be essential for transporter function by activating it and stabilizing its structure. PMID: 27302065
  9. High Glut3 expression is associated with gastric cancer. PMID: 26643879
  10. GLUT3 and OCT4 expression were correlated suggesting that Human embryonic stem cells self-renewal is regulated by the rate of glucose uptake. PMID: 26639784
  11. Using lipidic cubic phase crystallization and microfocus X-ray diffraction, we determined the structure of human GLUT3 in complex with D-glucose at 1.5 A resolution in an outward-occluded conformation PMID: 26176916
  12. We observed a strong relationship between characteristics of adaptive change to hypoxia, GLUT3 expression among birth weight-discordant twin PMID: 25728965
  13. The SLC2A3 duplication was the most frequent CNV detected and the only significant finding in our combined analysis , indicating that the SLC2A3 duplication might serve as a genetic modifier of congenital heart defect PMID: 25892112
  14. Glut3 is a downstream target of mTORC1, and it is critical for oncogenic mTORC1-mediated aerobic glycolysis and tumorigenesis. PMID: 25578782
  15. Data from human genetic analysis, molecular biology and a Drosophila Huntington's disease (HD) model strongly support the hypothesis that increased dosage of SLC2A3 ameliorates HD phenotypes. PMID: 24452335
  16. Patients after coronary artery bypass graft surgery, who have low GLUT3 levels are at increased risk for new-onset postoperative atrial fibrillation. PMID: 24463787
  17. deletions and single-nucleotide variations involving the GLUT3 gene that may be associated with increased susceptibility to myelomeningocele (MM). PMID: 24813597
  18. study presents discovery and characterization of a large copy-number variant (CNV) within the chr12p13.31 interval; genotyping of this CNV in multiple population samples produced evidence that a deletion spanning the SLC2A3 gene confers substantial protection against rheumatoid arthritis PMID: 24178905
  19. Decreased miR-106a in GBM tissues and conferred a poor survival of GBM patients. SLC2A3 was identified as a core target of miR-106a in GBM cells. PMID: 24124917
  20. Data suggest that expression of GLUT3/SLC2A3 (but not GLUT1 or GLUT4) is up-regulated in placenta in pregnancies complicated by intrauterine growth restriction (with expression of GLUT3 in cytotrophoblasts greater than in syncytiotrophoblasts). PMID: 24011442
  21. sensitive and specific marker for embryonal carcinomas and yolk sac tumors PMID: 23343953
  22. Nutrient restriction contributes to tumor progression by enriching for brain tumor initiating cells (BTICs); BTICs outcompete for glucose uptake by co-opting the high affinity neuronal glucose transporter, type 3 (Glut3, SLC2A3). PMID: 23995067
  23. GLUT3 shows increasing promoter methylation across gestation. PMID: 22901689
  24. The level of GLUT3 is correlated with the level of full-length CREB in Alzheimer brain samples. PMID: 23341039
  25. hypoxia-induced increases in glucose uptake through GLUT3 are important for lipid synthesis in macrophages, and may contribute to foam cell formation in hypoxic regions of atherosclerotic lesions. PMID: 22876317
  26. Data show that elevated CAV1 upregulates glucose uptake and ATP production through HMGA1-mediated GLUT3 transcription, suggesting that CAV1 may render tumor cells growth advantages by enhancing aerobic glycolysis. PMID: 22706202
  27. We clarified that early hypoxia-responsive genes are functionally associated with glycolysis, including SLC2A3. PMID: 22645302
  28. High GLUT3 is associated with endometrial and breast cancers. PMID: 22270867
  29. miR-195-5p is a novel and also the first identified miRNA that targets GLUT3, and the aberrant decreased expression of miR-195-5p and consequent GLUT3 up-regulation may contribute to bladder carcinogenesis. PMID: 22265971
  30. GLUT3 is present in the syncytial microvillous membrane early in gestation and decreases thereafter, supporting the idea that GLUT3 is of greater importance for glucose uptake early in gestation. PMID: 22000473
  31. Human glucose transporter type 3 (GLUT3)in the brain exhibits water channel properties PMID: 22113212
  32. Results suggest a possible transregulation effect on SLC2A3, which might lead to glucose deficits in dyslexic children and could explain their attenuated mismatch negativity in passive listening tasks. PMID: 19786962
  33. DNA-damaging agents reduce GLUT3 expression in cancer cells through activation of the MEK-ERK pathway independently of p53, leading to cell death or apoptosis PMID: 20870738
  34. In pluripotent stem cells and in human cancer disease, podocalyxin may function in part to regulate and maintain the cell surface expression of the glucose-3-transporter. PMID: 20599725
  35. expression of HIF-1alpha and GLUT-3 in glioma was correlated significantly with tumors' pathological grade, which can be taken as a pair of useful markers for predicting the biological behavior of glioma PMID: 19782666
  36. GLUT3 is expressed by normal articular chondrocytes. PMID: 11991658
  37. Restricted to regenerating muscle fibres and nerves in adult human muscle. GLUT3 may be important for glucose supply in fetal muscle fibres and regenerating adult muscle fibres. PMID: 12397394
  38. Embedded in microvillous (maternal-facing) and basal (fetal-facing) membranes of syncytiotrophoblast, main placental barrier layer. (review) PMID: 12583599
  39. in platelets glucose transport through GLUT3 is regulated by changes in surface expression and affinity modulation, which are both under control of PKB PMID: 16049004
  40. Regulation of GLUT3 and glucose uptake by the cAMP signallling pathway in the breast cancer cell line ZR-75 is reported. PMID: 17559076
  41. IGF-1 plays a role in maintaining muscle GLUT3 expression and basal glucose uptake via the transcriptional factor Sp1. PMID: 17920708
  42. GLUT3 expression was studied in normal and degenerate intervertebral discs. PMID: 18172662
  43. In hyperthyroidism: 1) basal abundance of GLUT3 and GLUT4 on the plasma membrane is increased and 2) the sensitivity of the recruitment of GLUT3 and GLUT4 transporters on the plasma membrane in response to IGF-I is increased PMID: 18299470
  44. GLUT-3 gene expression level was high in head and neck carcinoma (HNCs), and its expression was associated with an increased incidence of lymph node metastasis of HNCs. PMID: 18401196
  45. differential expression of Glut-3 by benign and malignant melanocytic lesions PMID: 18764953
  46. Sertoli cells express GLUT1 and GLUT3 throughout pubertal development. PMID: 18802725
  47. the activation of AMP-activated protein kinase and its regulation of cell surface GLUT3 expression is critical in mediating neuronal tolerance to excitotoxicity PMID: 19261894
  48. The expression pattern of GLUT3 is reported in newly diagnosed esophageal adenocarcinoma by means of immunohistochemistry. PMID: 19554504
  49. The neuronal glucose transporter 3 was decreased to a bigger extent in Type 2 Diabetes Mellitus brain than in Alzheimer's disease brain. PMID: 19659459

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Perikaryon. Cell projection.
Protein Families
Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family, Glucose transporter subfamily
Tissue Specificity
Highly expressed in brain. Expressed in many tissues.
Database Links

HGNC: 11007

OMIM: 138170

KEGG: hsa:6515

STRING: 9606.ENSP00000075120

UniGene: Hs.419240

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