SMARCE1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA242217A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), K562 whole cell lysate(30µg), whole cell lysate(30µg), U-251MG whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), A549 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: SMARCE1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 47 kDa Observed band size: 57 kDa Exposure time：3min IHC image of CSB-RA242217A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA242217A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA242217A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA242217A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q969G3

Target Names

SMARCE1

Alternative Names

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 (BRG1-associated factor 57) (BAF57), SMARCE1, BAF57

Species Reactivity

Human

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

18A8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SMARCE1, also known as BAF57, serves as an essential subunit of the SWI/SNF chromatin remodeling complex, where it plays a critical role in regulating gene expression through nucleosome repositioning. This protein participates in diverse cellular processes including transcriptional regulation, DNA repair, and cell cycle control, making it a valuable target for researchers investigating chromatin dynamics, epigenetic mechanisms, and cancer biology.

This recombinant monoclonal antibody, generated in rabbit using a synthesized peptide from human SMARCE1, offers the consistency and reliability that demanding experiments require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring reproducible results across long-term studies and multi-site collaborations.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis detects SMARCE1 in a broad panel of human cell lines including HeLa, HepG2, SH-SY5Y, K562, U-251MG, A549, and Jurkat cells, with the observed band appearing at approximately 57 kDa rather than the predicted 47 kDa—a difference likely attributable to post-translational modifications such as phosphorylation or glycosylation that are characteristic of chromatin-associated proteins. Immunohistochemistry applications have been validated in paraffin-embedded human breast cancer and testis tissues, while immunofluorescence studies in A549 cells reveal clear nuclear localization consistent with SMARCE1's chromatin-associated function. Flow cytometry validation in SH-SY5Y cells further expands the experimental flexibility this antibody provides.

Whether investigating SWI/SNF complex function in normal development or exploring its dysregulation in malignancies where SMARCE1 alterations have been implicated, this antibody delivers the specificity and versatility needed for meaningful discoveries.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells.

Gene References into Functions

High SMARCE1 expression is associated with eventual relapse and metastasis in breast cancer. PMID: 28377514
miR-29a promotes hepatitis B virus (HBV) replication and expression through regulating SMARCE1 in HBV-infected HepG2.2.15 cells. PMID: 28740345
We report here three additional individuals with clinical features consistent with CSS and alterations in SMARCE1, one of which is novel. The probands all exhibited dysmorphic facial features, moderate developmental and cognitive delay, poor growth, and hypoplastic digital nails/phalanges, including digits not typically affected in the other genes associated with CSS. PMID: 27264197
SMARCE1 mutational hits, including novel SMARCE1 mutations, were found in six of eight tested patients with clear cell meningioma PMID: 27891692
An exhaustive analysis of the BAF57 molecular and biochemical properties, cellular functions, loss-of-function phenotypes in living organisms and pathological manifestations in cases of human mutations. [review] PMID: 27149204
a family with a pediatric CCM patient and an adult CCM patient and several asymptomatic relatives carrying a germline SMARCE1 mutation. PMID: 26803492
Addition of the EGFR inhibitor gefitinib restores the sensitivity of SMARCE1-knockdown cells to MET and ALK inhibitors in NSCLCs. Our findings link SMARCE1 to EGFR oncogenic signaling and suggest targeted treatment options for SMARCE1-deficient tumors. PMID: 25656847
The results suggested that BAF57 is involved in ovarian cancer cell growth and sensitivity to anticancer agents, and that BAF57 may be a target for ovarian cancer therapy. PMID: 25611552
BAF complex gene SMARCE1 is mutated in Coffin-Siris syndrome patients. PMID: 25081545
Genotype-phenotype correlation of Coffin-Siris syndrome caused by mutations in SmarCE1 gene. PMID: 25168959
Since both TTF1 and SMARCE1 are involved in chromatin remodeling, our results imply an epigenetic regulatory mechanism for T-cell recruitment that invites deciphering. PMID: 24880093
these results demonstrate that loss of SMARCE1 is relevant to cranial as well as spinal meningiomas PMID: 25143307
Data indicate that BAF57 deregulation predisposes to metastasis. PMID: 23493350
Our findings identify multiple-spinal-meningioma disease as a new discrete entity and establish a key role for the SWI/SNF complex in the pathogenesis of both meningiomas and tumors with clear-cell histology. PMID: 23377182
mutations in BAF57 could impinge on several oncogenic signaling pathways contributing to the origin and/or development of breast cancer. PMID: 21465167
BAF57 expression was significantly associated with the surgical stage, grade of the tumor, myometrial invasion, lympho-vascular space invasion and lymph node metastasis in 111 endometrial carcinomas. PMID: 22419023
Knockdown of BAF57 resulted in an accumulation of cells in the G(2)-M phase, inhibition of colony formation, and impaired growth in soft agar. Knockdown of BAF57 also caused transcriptional misregulation of various cell cycle-related gene. PMID: 20460533
BAF57-mediated cell death is associated with up-regulation of proapoptotic genes including the tumor suppressor familial cylindromatosis (CYLD), which is found to be a direct target of BAF57. PMID: 16135788
BAF57 is a critical regulator of estrogen receptor function in breast cancer cells PMID: 16769725
Ability of SMARCE 1 in modulating the replication efficiency of HBV. PMID: 17669635

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Involvement in disease

Meningioma (MNGMA); Coffin-Siris syndrome 5 (CSS5)

Subcellular Location

Nucleus.

Database Links

HGNC: 11109

OMIM: 603111

KEGG: hsa:6605

STRING: 9606.ENSP00000323967

UniGene: Hs.743978

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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