Product Details

Uniprot No.

Q13596

Target Names

SNX1

Alternative Names

Sorting nexin-1, SNX1

Species Reactivity

Human

Immunogen

A synthesized peptide from human SNX1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8E11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Sorting nexin-1 plays a central role in intracellular trafficking, functioning as a key component of the retromer complex that mediates retrograde transport from endosomes to the trans-Golgi network. This protein's involvement in cargo sorting and membrane remodeling makes it an important target for researchers investigating endosomal dynamics, receptor recycling, and neurodegenerative disease mechanisms where trafficking defects contribute to pathology.

This recombinant monoclonal antibody, generated from clone 8E11, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, researchers can expect reliable performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation data demonstrates robust performance across multiple applications. Western blot analysis detects SNX1 in diverse human cell lines including HT-29, A431, Jurkat, HL-60, and HEK293T lysates, with an observed band at approximately 74 kDa. This migration above the predicted 59 kDa molecular weight likely reflects post-translational modifications such as phosphorylation, which is known to regulate SNX1 function. Immunofluorescence staining in HeLa cells reveals the expected cytoplasmic localization pattern consistent with endosomal distribution, while flow cytometry validation confirms utility for quantitative single-cell analysis of SNX1 expression levels.

The flexibility to move between biochemical, imaging, and cytometric approaches using a single well-characterized reagent streamlines experimental design for studies examining endosomal trafficking, autophagy regulation, or receptor signaling dynamics. This antibody serves researchers working across cell biology, neuroscience, and cancer biology where understanding intracellular sorting mechanisms informs both basic discovery and therapeutic development.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1) and Shiginella dysenteria toxin stxB. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking.

Gene References into Functions

Data suggest that GLP1R signaling in pancreatic beta-cells leading to insulin secretion involves interactions of GLP1R with HIP1, SNX1, and SNX27; HIP1 appears to regulate coupling of cell surface GLP1R activation with endocytosis; SNX1 and SNX27 appear to control balance between GLP1R plasma membrane recycling and lysosomal degradation. PMID: 29284659
SNX1 was down-regulated in CRC and associated with poor prognosis. Our data also showed that SNX1 inhibited the proliferation of CRC cells and increased the sensitivity to the most commonly used drugs for CRC. PMID: 26643894
miR-95 functions as an oncogene role in non-small cell lung cancer cells by directly targeting SNX1. PMID: 24835695
Results show that suppression of EGFR membrane recycling by SNX1 appears to be critical for the activation of EGFR /PI3K/AKT signaling pathway in human lung cancer cells. PMID: 25653196
we suggest that SNX1 is involved in the negative regulation of ligand-induced EGFR phosphorylation and mediates EGFR/pEGFR trafficking out of early endosomes PMID: 22859339
SNX1 has a crucial role in D(5)R trafficking and SNX1 depletion results in D(5)R dysfunction and thus may represent a novel mechanism for the pathogenesis of essential hypertension PMID: 23152498
This study found that significant evidence of association with SNX1 (VEGAS p = 0.035) in patient with Alzheimer disease. PMID: 22673115
SNX4, but not SNX1 and SNX8, is associated with the Rab11-recycling endosomes and that a high frequency of SNX4-mediated tubule formation is observed as endosomes undergo Rab4-to-Rab11 transition. PMID: 21973056
Results demonstrate that miR-95 increases proliferation by directly targeting SNX1, defining miR-95 as a new oncogenic miRNA in CRC. PMID: 21427358
SNX1 and SNX2 interact with Kalirin-7. Overexpression of SNX1 or SNX2 and Kalirin-7 partially redistributes both SNXs to the plasma membrane, and results in RhoG-dependent lamellipodia formation. PMID: 20604901
These data describe a novel function of SNX1 in the regulation of P2Y(1) receptor recycling and suggest that SNX1 plays multiple roles in endocytic trafficking of G-protein coupled receptors. PMID: 20070609
controls down-regulation of protease-activated receptor-1 PMID: 12058063
PX domain-dependent/early endosomal association of SNX1 is important for its ability to regulate the targeting of internalized epidermal growth factor receptor for lysosomal degradation PMID: 12198132
identified sorting nexin 1 (SNX1) as a novel partner of enterothelin-1 (ENT-1); show that Ent-1 and SNX1 co-eluted in macromolecular complexes containing part of epidermal growth factor receptor(EGFR) PMID: 12657642
SNX1 associates with a microdomain of the early endosome where it regulates tubular-based endosome-to-trans Golgi network retrieval of the cation-independent mannose-6-phosphate receptor. PMID: 15498486
NMR structure of the PX domain of SNX1 reveals an overall fold that is similar to high-affinity PX domains PMID: 15673616
HkRP1 is involved in the process of tubulation of sorting nexin-1 positive membranes from early endosome subdomains PMID: 15882442
These findings establish an essential role for endogenous SNX1 in sorting activated PAR1 to a distinct lysosomal degradative pathway that is independent of retromer, Hrs, and Tsg101. PMID: 16407403
Overexpression of SNX1 is able to attenuate the effect of SNX5 on epidermal growth factor (EGF) receptor degradation. PMID: 16487940
These observations indicate that the mammalian retromer complex assembles by sequential association of SNX1/2 and Vps26-Vps29-Vps35 subcomplexes on endosomal membranes and that SNX1 and SNX2 play interchangeable but essential roles. PMID: 17101778
The loss of SNX1 may play a significant role in the development and aggressiveness of human colon cancer, at least partially through the mechanism of increased signaling from endosomes. PMID: 17145813
Together, these findings demonstrate a role for SNX1 in retrieval of E-cadherin from a degradative endosomal pathway and in membrane trafficking pathways that regulate E-cadherin recycling. PMID: 17502486
sortilin and mannose-6-phosphate receptors recycle to the TGN in SNX1-dependent carriers, which we named endosome-to-TGN transport carriers PMID: 18088323

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Subcellular Location

Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium.

Protein Families

Sorting nexin family

Database Links

HGNC: 11172

OMIM: 601272

KEGG: hsa:6642

STRING: 9606.ENSP00000453785

UniGene: Hs.188634

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Code	CSB-RA986243A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(30µg), A431 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), HL-60 whole cell lysate(30µg), HEK293T whole cell lysate(30µg) All lanes: Sorting nexin 1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 59 kDa Observed band size: 74 kDa Exposure time：2min Immunofluorescence staining of Hela cell with CSB-RA986243A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA986243A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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