Code | CSB-RA633872A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
To prepare the SOD2 recombinant monoclonal antibody, a combination of protein technology and DNA recombinant technology was employed. Initially, mice were immunized with a synthesized peptide derived from human SOD2. After a certain period, the spleen was removed from the mice under sterile conditions. Total RNA was extracted from spleen cells and used to synthesize cDNA, which acted as the template for PCR amplification of the SOD2 antibody gene. The obtained SOD2 antibody gene was then inserted into a vector and transfected into host cells for culture. The SOD2 recombinant monoclonal antibody was purified from the supernatant of cell culture using affinity chromatography. It has undergone rigorous verification and is suitable for detecting human and rat SOD2 proteins in ELISA, WB, and IHC experiments.
The SOD2 protein, also known as manganese superoxide dismutase (MnSOD), is an enzyme that plays a critical role in the cell's antioxidant defense system. It catalyzes the conversion of superoxide radicals into hydrogen peroxide and molecular oxygen, which prevents the accumulation of superoxide radicals, preventing damage to the mitochondrial membrane and the DNA inside the mitochondria. SOD2 has been shown to play a role in other cellular processes, such as regulating the activity of transcription factors and influencing cell signaling pathways. The dysregulation of SOD2 has been implicated in a variety of diseases, including cancer, neurodegenerative disorders, and cardiovascular disease.
Applications : western blot analysis
Sample type: cell
Review: The protein expression levels were determined by western blot analysis. Effect of PTX on SOD1, SOD2 and CAT protein expression in rat lung tissue following chlorine exposure.
By Anonymous