Code | CSB-RA782732A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
To create a recombinant monoclonal antibody against SOD2 acetylated at K68, CUSABIO's approach began with the immunization of a rabbit using a synthesized peptide derived from human SOD2 protein. Subsequent steps involved isolating B cells from the immunized rabbit and extracting RNA from these cells. The extracted RNA was reverse-transcribed into cDNA, which was utilized as a template for extending SOD2 antibody genes using degenerate primers. These extended SOD2 antibody genes were integrated into a plasmid vector and introduced into host cells for expression. The acetyl-SOD2 (K68) recombinant monoclonal antibody was purified from the cell culture supernatant through affinity chromatography and evaluated for its utility in ELISA and IHC applications. It only reacts with human SOD2 protein acetylated at K68.
SOD2 is an enzyme responsible for scavenging superoxide radicals within the mitochondria, thereby protecting cells from oxidative damage. The acetylation of SOD2 at K68 is known to regulate enzymatic activity, protect against oxidative stress, as well as participate in mitochondrial function and cellular signaling.
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