STX3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA280045A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), K562 whole cell lysate(30µg), whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: Syntaxin 3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 33 kDa Observed band size: 36 kDa Exposure time：3min Overlay Peak curve showing A431 cells stained with CSB-RA280045A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q13277

Target Names

STX3

Alternative Names

Syntaxin-3, STX3, STX3A

Species Reactivity

Human

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

20C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Syntaxin-3 plays a central role in membrane trafficking and vesicle fusion events, particularly in polarized epithelial cells where it facilitates the targeted delivery of proteins to the apical membrane. This SNARE protein is essential for maintaining cellular polarity and has garnered significant research interest for its involvement in secretory pathways, neurotransmitter release, and epithelial barrier function.

This recombinant monoclonal antibody against human STX3 offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies and collaborative projects.

Validation testing demonstrates robust performance across multiple experimental platforms. Western blot analysis at 1:1000 dilution reveals specific detection of Syntaxin-3 across a diverse panel of human cell lines, including HeLa, HepG2, SH-SY5Y, K562, COLO-205, HEK293, MCF-7, and A431 cells. The observed band size of 36 kDa, slightly higher than the predicted 33 kDa molecular weight, likely attributes to the mobility deviation of STX3 in SDS-PAGE (influence of amino acid composition/conformation) or minor post-translational modifications (such as low-abundance glycosylation/phosphorylation). Flow cytometry applications have been validated using A431 cells with clear positive signal separation from isotype controls, with recommended dilutions ranging from 1:50 to 1:200.

Whether investigating epithelial polarity mechanisms, secretory pathway dynamics, or SNARE complex assembly, this antibody provides a reliable tool for researchers studying membrane trafficking biology. Its validated performance in both protein detection and single-cell analysis applications offers flexibility for comprehensive characterization of STX3 expression and localization in your experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Potentially involved in docking of synaptic vesicles at presynaptic active zones.

Gene References into Functions

Stx3S is differentially expressed in normal human tissues, during epithelial cell polarization, and in breast cancer versus normal breast tissue. Inhibition of endogenous Stx3S expression alters the expression of cancer-associated genes and promotes cell proliferation. PMID: 29475951
mechanism study shows that STX3 binds to PTEN and increases PTEN ubiquitination and degradation, thus leading to activation of the PI3K-Akt-mTOR signaling. Therefore, STX3 promotes the growth of breast cancer cells by regulating the PTEN-PI3K-Akt-mTOR signaling. PMID: 29408595
we report for the first time a missense mutation of a novel lens specific gene STX3 in a phenotype associating autosomal recessive congenital cataract and intellectual disability. PMID: 25358429
GLUT5 required an interaction cascade of Rab11, Myo5B, Slp4a, Munc18-2, and Vamp7 with Stx3. PMID: 26553929
Rab11a-knockdown cells displayed mislocalization of STX3 and the presentation of microvilli in the basolateral compartment PMID: 25673875
the secretion of IL-1alpha, IL-1beta, IL-12b, and CCL4 occurs during gelatinase degranulation, a process controlled by STX3. PMID: 25548252
The results showed that there was reduction in aggregation propensity of syntaxin-3 with point mutation at Val286. PMID: 25630102
hcmv-miR-US33-5p-mediated inhibition of HCMV DNA synthesis and of viral replication are specifically mediated by down-regulation of STX3 expression. PMID: 25583387
loss of STX3 function causes variant microvillus inclusion disease PMID: 24726755
syntaxin3 has a latent prosurvival function and is involved in maintenance of homeostasis in epidermal keratinocytes PMID: 24171760
In insulin secretion, SYN-3 plays a role in the mediation of newcomer SG exocytosis and SG-SG fusion that contributes to biphasic GSIS. PMID: 23132338
siRNA knockdown (KD) of syntaxins 3 and 4 in HeLa cells reduced cell surface expression of alpha5beta1 and alpha3beta1 integrins PMID: 21720706
STX-3 and SNAP-23 are crucial for the release of all chemokines in mature human mast cells PMID: 21981832
These results demonstrate a function for STX3 in human cytomegalovirus morphogenesis, and unravel a new role for this SNARE protein in late endosomes/lysosomes compartments. PMID: 21371234
syntaxin 3 interacts with CFTR in vivo. PMID: 20844248
Munc18b is functionally coupled to the assembly of exocytic SNARE complexes and increases exocytosis by interacting with the N-peptide and closed-conformation C-terminus of Stx3, thereby neutralizing the secretion-inhibitory effect of this SNARE. PMID: 20695848
Data show that the BCG phagosome is relatively depleted in LAMP-2, NPC1, flotillin-1, vATPase, and syntaxin 3. PMID: 19815536

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Subcellular Location

Membrane; Single-pass type IV membrane protein.

Protein Families

Syntaxin family

Database Links

HGNC: 11438

OMIM: 600876

KEGG: hsa:6809

STRING: 9606.ENSP00000338562

UniGene: Hs.180711

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Synaptic vesicle cycle

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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