Product Details

Uniprot No.

Target Names

SUMO4

Alternative Names

dJ281H8.4 antibody; IDDM5 antibody; Small ubiquitin like modifier 4 protein antibody; Small ubiquitin-like protein 4 antibody; Small ubiquitin-related modifier 4 antibody; SMT3 suppressor of mif two 3 homolog 2 antibody; SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae) antibody; SMT3H4 antibody; SUMO 4 antibody; SUMO-4 antibody; SUMO4 antibody; SUMO4_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human SUMO4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

19F12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SUMO4 belongs to the small ubiquitin-like modifier family, a group of proteins that regulate cellular processes through post-translational conjugation to target substrates. Unlike other SUMO family members, SUMO4 expression is predominantly restricted to specific tissues and has garnered significant research interest due to its genetic association with type 1 diabetes susceptibility. The protein plays important roles in modulating immune responses and stress signaling pathways, making it a valuable target for investigators studying autoimmune disease mechanisms and cellular stress responses.

This recombinant monoclonal antibody, clone 19F12, offers the consistency and reproducibility that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies. The antibody was raised against a synthetic peptide derived from human SUMO4 and purified by affinity chromatography for optimal specificity.

Validation across multiple detection platforms demonstrates this antibody's versatility in your research applications. Immunohistochemistry studies in paraffin-embedded human colorectal cancer tissue show clear staining at dilutions of 1:50–1:200, while immunofluorescence analysis in HeLa cells reveals distinct signal with DAPI counterstaining for subcellular localization studies. Flow cytometry validation using 786-O renal carcinoma cells confirms reliable detection of intracellular SUMO4 following fixation and permeabilization, with clear separation from isotype control.

Whether investigating SUMO4's role in immune regulation, exploring its connections to metabolic disease, or examining sumoylation dynamics in cancer biology, this antibody provides the technical foundation for robust, reproducible cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I.

Gene References into Functions

meta-analysis demonstrated that the G allele of the SUMO4 M55V polymorphism could be a susceptible risk locus to type 2 diabetes mellitus, mainly in the Chinese population, while the association in other ethnic population needs to be further validated PMID: 28494838
Both donor and recipient SUMO4 rs237025 polymorphisms contribute to the development of new-onset diabetes mellitus after liver transplantation. PMID: 28689037
Results show that SUMO-4 is expressed in normal placental development. SUMO-4 expression was increased in pre-eclamptic placentas and in models of oxidative stress and hypoxic injury suggesting that SUMO-4 hyper-SUMOylation may be a potential post-translational mechanism in the stressed pre-eclamptic placenta. PMID: 28545138
study suggests that SUMO4 c.163 G to A polymorphism is associated with the susceptibility to diabetic nephropathy in north Indian subjects with type 2 diabetes PMID: 27055882
SUMO4 M55V polymorphism and type-2 diabetes were significantly associated with a possible SUMO4 region to type-2 diabetes susceptibility. PMID: 25189908
No association between SUMO4 gene polymorphisms and acoronary artery disease with and without type 2 diabetes mellitus. PMID: 23042402
SUMO4 Met55Val variant was associated with increased insulin resistance in Chinese patients with newly diagnosed type 2 diabetes. PMID: 21671169
study demonstrates significant associations of SUMO4 M55V polymorphism with type 1 diabetes mellitus in Asian and Caucasian population and with type 2 diabetes mellitus in Asian population PMID: 22936652
The C438T polymorphism in the SUMO4 gene is associated with significantly increased risk of papulopustular skin lesions in HLA-B51-positive Behcet's disease patients. PMID: 21901353
This meta-analysis indicates that the SUMO4 M55V polymorphism confers susceptibility to type I diabetes in Asians and Europeans. PMID: 22884980
the association between small ubiquitin-like modifier 4 (SUMO4) gene polymorphisms and type 2 diabetes mellitus PMID: 22425950
Letter: report SUMO4 polymorphisms associated with protective effect in Tunisian rheumatoid arthritis patients. PMID: 21906435
The SUMO4 gene single nucleotide polymorphism variant is not associated with susceptibility of the type 2 diabetes polymorphism. PMID: 20728233
SUMO4 gene polymorphisms may be involved in the development of skin lesions, vascular Behcet's disease, as well as the severity of the disease. PMID: 20868570
SUMO4 gene M55V variant is associated with the genetic susceptibility of type 1 diabetes mellitus (T1D) in Chinese children. PMID: 21158221
This study suggests that in Taiwan the SUMO4 Met 55Val polymorphism is associated with susceptibility to T2DM and Type 2 diabetic patients with GG genotype have worse glycemic control. PMID: 19915388
Observational study of gene-disease association. (HuGE Navigator) PMID: 20728233
Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20448286
Meta-analysis of gene-disease association. (HuGE Navigator) PMID: 20518843
total of 2317 families were genotyped for 15 SNPs in the SUMO4 region. PMID: 19956095
we report evidence for an association between T1D and multiple single-nucleotide polymorphisms in 197 kb of genomic DNA in the IDDM5 interval. PMID: 15247916
SUMO4 sumoylation may play an important role in the regulation of intracellular stress PMID: 16236267
The M55V polymorphism was significantly associated with type 1 diabetes in Asian populations . PMID: 16306380
results indicate that the small ubiquitin-like protein 4(SUMO4) is a more common autoimmune disease gene and a supplementary risk factor to type 1 diabetes PMID: 16735488
SUMO4 SNP did not significantly influence predisposition to and features of rheumatoid artritis, in contrast to previous genetic and functional evidence that suggested its involvement. PMID: 16755651
Genetic and functional evidence is provided supporting SUMO4 as a type 1 diabetes mellitus susceptibility gene. PMID: 17130563
No significant association was found between SUMO4 M55V and LADA. PMID: 17130565
SUMO4 gene methionine-valine variant is associated with severity of diabetic nephropathy in patients with type 2 diabetes. PMID: 17229939
Our results show that Asian-Indians with T1DM are different from other Asian populations. Asian-Indians show more similarity to Caucasians with respect to the association of SUMO4 M55V variant in T1DM. PMID: 17373940
contribution of the SUMO4 Met55Val polymorphism to both type 1 and type 2 diabetes susceptibility in the Japanese population PMID: 17374705
SUMO4 protein appears to be either in extremely low abundance in human kidney or HEK293 cells or it is not present at all.It remains possible that SUMO4 protein is more abundant in other cell types or can be induced by hormonal or environmental challenges PMID: 17459725
we conclude that SUMO4 M55V is associated with type I diabetes melitus in association with high-risk HLA-DR3 and DR4, but not by itself. PMID: 17554341
study of the prevalence of four polymorphisms,CTLA-4 -318 C/T, 159 C/T, 3' STR and SUMO4 163 AG in latent autoimmune diabetes in adults; no association with either of the polymorphisms has been found PMID: 17558709
reported a positive association of the SUMO4 M55V variant with diabetic nephropathy in an Asian cohort of 430 patients with type 2 diabetes PMID: 17660269
data indicate that the M55V polymorphism in the small ubiquitin-like protein 4(SUMO4) gene is associated with a reduced risk of diabetic retinopathy in type 1 diabetes PMID: 17926234
we will update the genetic evidence supporting SUMO4 as a T1D susceptibility gene and discuss the possible explanations for the discrepant associations observed in Caucasians vs Asians.[review] PMID: 17990297
no associations of the SUMO4 rs237025 A>G polymorphism with the susceptibility of psoriasis were detected. PMID: 18330586
SUMO4 +438 C allele is associated with susceptibility to Behcet's Disease (BD) in HLA-B51 negative Chinese Han patients, while the AGAT haplotype is protectively associated with BD in HLA-B51 negative patients PMID: 18657476
These data not only confirmed previously published data, but also provided additional evidence suggesting a role for SUMO4 sumoylation in the regulation of intracellular stress. PMID: 18708028
This study examines whether small ubiquitin-like modifier 4 (SUMO4) polymorphisms were associated with Vogt-Koyanagi-Harada (VKH) syndrome in the Chinese Han population. PMID: 19122825
SUMO4 may act as a negative feedback regulator to prevent excessive activation of NFkappaB. PMID: 19222990
Association of the polymorphism of SUMO4 with risk of coronary heart disease in type 2 diabetes is suggested. PMID: 19410319
Observational study of gene-disease association. (HuGE Navigator) PMID: 19915388
Observational study of gene-disease association. (HuGE Navigator) PMID: 19410319
Observational study of gene-disease association. (HuGE Navigator) PMID: 19122825
Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 18466472
Observational study of gene-disease association. (HuGE Navigator) PMID: 18466531
Observational study of gene-disease association. (HuGE Navigator) PMID: 18330586
Observational study of gene-disease association. (HuGE Navigator) PMID: 12901503
Observational study of gene-disease association. (HuGE Navigator) PMID: 15730420

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Protein Families

Ubiquitin family, SUMO subfamily

Tissue Specificity

Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen.

Database Links

HGNC: 21181

OMIM: 600320

KEGG: hsa:387082

STRING: 9606.ENSP00000318635

UniGene: Hs.657168

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Code	CSB-RA101412A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA101412A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HeLa cell with CSB-RA101412A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 786-O cells stained with CSB-RA101412A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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SUMO4 Recombinant Monoclonal Antibody

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Target Background