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SYP Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA023024MA1HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: A549 whole cell lysatee
    All lanes: SYP antibody at 1:500
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 34 kDa
    Observed band size: 34 kDa
  • IHC image of CSB-RA023024MA1HU diluted at 1:200 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of Hela cell with CSB-RA023024MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Product Details

Uniprot No.
P08247
Target Names
SYP
Alternative Names
SYP Recombinant Monoclonal Antibody
Species Reactivity
Human
Immunogen
Recombinant Human SYP protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
mFc2a
Clone No.
2B2
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Synaptophysin serves as one of the most widely used markers for synaptic vesicles and neuroendocrine differentiation, making it indispensable for neuroscience research and diagnostic pathology. This integral membrane glycoprotein localizes to presynaptic vesicles throughout the nervous system and plays essential roles in synaptic vesicle biogenesis and neurotransmitter release. Its consistent expression pattern has established synaptophysin as a gold-standard marker for identifying neuronal and neuroendocrine tissues.

This recombinant monoclonal antibody, clone 2B2, offers the reproducibility that demanding research applications require. Because the antibody sequence is defined and produced through recombinant technology, you can expect consistent performance across experiments and between lots, eliminating the variability that can complicate long-term studies or multi-site collaborations. The mouse host and mFc2a isotype provide compatibility with standard secondary detection systems.

Validation across multiple techniques demonstrates this antibody's experimental flexibility. Western blot analysis of A549 whole cell lysate detects a band at the predicted 34 kDa molecular weight, confirming target specificity at dilutions between 1:500 and 1:2000. Immunohistochemistry testing in paraffin-embedded human colorectal cancer tissue shows reliable staining at 1:200 dilution following citrate buffer antigen retrieval, while immunofluorescence analysis in HeLa cells demonstrates clear signal suitable for subcellular localization studies.

Whether you are investigating synaptic plasticity, characterizing neuroendocrine tumors, or examining neuronal differentiation in cell culture models, this antibody provides a dependable tool validated in human samples across complementary detection platforms. The affinity-purified preparation arrives ready for immediate use in your neuroscience research workflows.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

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Customer Reviews and Q&A

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Target Background

Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Gene References into Functions
  1. Syp can be quantified on circulating tumor cells and that Syp expression correlates with resistance to abiraterone and enzalutamide in castration resistant prostate cancer. PMID: 29289429
  2. Report of a novel SYP mutation identified in a patient with a severe neurodevelopmental disorder. Mutation does not impact on synaptic vesicles endocytosis or trafficking of common cargoes. However it is unable to support sybII retrieval in synaptophysin knockout neurons. It fully restores plasma membrane levels of sybII, suggesting that activity-dependent trafficking of sybII is specifically affected by this mutation. PMID: 28887151
  3. The expression of MAP2 and SYP proved most relevant to neurocognitive functioning in HIV-infected patients. PMID: 26637429
  4. Synaptophysin-positive epithelial proliferations suggest breast neoplasms. PMID: 28630050
  5. Suggest that INSM1 is valuable for identification of the high-grade thoracic neuroendocrine carcinomas that are sometimes negative for synaptophysin, chromogranin, and CD56. PMID: 28719469
  6. High synaptophysin expression is associated with neuroendocrine differentiation in colorectal cancer. PMID: 28351413
  7. Study reveals the presence of two selected neuronal markers, MAP-2 and SYP mRNAs and protein expression, in eutopic endometrium and in endometriotic lesions. PMID: 26604067
  8. Anomalous expression of all intermediate filament proteins and synaptophysin was found in significant subsets of malignant melanoma. PMID: 26022451
  9. Docosahexaenoic acid-containing phosphatidylcholines and PSD-95 decrease after loss of synaptophysin and before neuronal loss in patients with Alzheimer's disease. PMID: 25410733
  10. This study demonstrated that synaptophysin was significantly lower in Down syndrome with Alzheimer disease compared with Down syndrome alone and similar to sporadic Alzheimer disease. PMID: 24927707
  11. The results of this study suggested that SYP may be primarily associated with ADHD-I and its genetic mechanism may be gender-specific. PMID: 23726717
  12. The paucity or absence of synaptophysin-positive cells in all three phenotypes of Barrett's mucosa might mirror a sequela of chronic inflammation caused by the particular pathogenic bacteria present in the immediate oesophageal microenvironment. PMID: 23763443
  13. Data suggest that multi-antibody assay of TTF1, Vimentin, p63 CD56, chromogranin and synaptophysin may be of special value, especially in diagnosing small biopsies. PMID: 23418554
  14. the author showed that, in cutaneous basal cell carcinoma, the expression of NCAM and c-KIT was high, PDGFRA was intermediate, and chromogranin A and synaptophysin was relatively low PMID: 23292839
  15. Depressed patients showed significant decreases in synaptophysin (SYN) and VGLUT1 expression. PMID: 23022470
  16. Lower levels of synaptophysin were found in the visual cortex of dementia with Lewy bodies patients compared to controls. PMID: 23242284
  17. Normal goblet cells found in duodenal adenomas express synaptophysin. PMID: 22843923
  18. The data of this study suggested that the common polymorphisms of the SYP gene appear not to play a major role in conferring susceptibility to schizophrenia. PMID: 22348818
  19. This is the first report of immunoreactivity for inhibin-alpha and synaptophysin in synovial sarcoma PMID: 22055401
  20. NCAM1, SYPT and CGA expressions are differently regulated by neuroendocrine phenotype-specific transcription factors in lung cancer cells. PMID: 22449227
  21. Finding that synaptophysin is produced by Paneth cells adds new information which might help to unravel the riddle of the ultimate biological significance of these puzzling cells. PMID: 22210727
  22. The expression of synaptophysin and GAD67 was downregulated in the basolateral nucleus of schizophrenics. PMID: 22099865
  23. Prescence of of APOEepsilon4 allele and MAPT H2 haplotype in frontotemporal lobar degeneration had a significant influence on the expression of synaptophysin and SNAP-25 PMID: 21073671
  24. Significant decreases in synaptophysin levels are observed in hippocampal neurons from Alzheimer's disease patients. PMID: 20847448
  25. during prenatal development synaptophysin expression increased gradually in the red nucleus PMID: 12077483
  26. we have identified a functional binding site for REST in the first intron of the human synaptophysin gene indicating that REST blocks human synaptophysin gene transcription through an intronic neuron-specific silencer element. PMID: 12492469
  27. AP180 had an overall expression similar to synaptophysin, the immunoreactivity for the two proteins did not always co-localize. PMID: 12493563
  28. This protein was found in cells of adrenal cortical neoplasms. PMID: 12575533
  29. expression and localization of chromogranin A (CgA), chromogranin B (CgB), synaptophysin, and insulin were ultrastructurally studied with the immunogold technique in porcine and human pancreatic islet neuroendocrine cells PMID: 12826904
  30. Synaptophysin immunoreactivity was significantly reduced in both the inner and outer molecular layers of the dentate gyrus (both p<0.01 by paired t-test), but not in the hilus. In contrast to patients with schizophrenia. PMID: 15694236
  31. Synaptophysin was absent near neurons bearing clusters of oligomer Abeta, suggesting that accumulated oligomer Abeta, but not fibrillar Abeta, is closely associated with synaptic failure, a major cause of cognitive dysfunction. PMID: 16721760
  32. Results describe patterns of synaptophysin protein and mRNA expression in the human hippocampal formation from birth to old age. PMID: 16807900
  33. Synaptophysin regulates micro-opioid receptor trafficking and signaling. PMID: 17005904
  34. Overexpression of Synaptophysin is associated with squamous carcinoma and adenocarcinoma of the lung PMID: 17724707
  35. synaptophysin levels slowly increased from birth until age 5 and then increased more rapidly to peak in late childhood around age 10 then decrease until the adult level was reached by age 16 PMID: 17916412
  36. Synaptophysin(SYN) was correlated with mini-mental status examination scores. Frontal cortex SYN protein levels may protect neuronal function in oldest-old individuals and reflect compensatory responses that may be involved with maintaining cognition. PMID: 18006193
  37. Chromogranin A-, serotonin-, synaptophysin- and vascular endothelial growth factor-positive endocrine cells and the prognosis of colorectal cancer: an immunohistochemical and ultrastructural study. PMID: 18771509
  38. Additional immunohistochemical detection of neuroendocrine differentiation (chromogranin-A, synaptophysin, and neural-cell adhesion molecule) in non-small cell lung cancer is presently not of prognostic importance. PMID: 19652998
  39. Chromogranin A, synaptophysin and CK 20 were not expressed in any basal cell carcinoma PMID: 19724850

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Involvement in disease
Mental retardation, X-linked 96 (MRX96)
Subcellular Location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
Protein Families
Synaptophysin/synaptobrevin family
Tissue Specificity
Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.
Database Links

HGNC: 11506

OMIM: 300802

KEGG: hsa:6855

STRING: 9606.ENSP00000263233

UniGene: Hs.632804

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