Product Details

Uniprot No.

P04183

Target Names

TK1

Alternative Names

Thymidine kinase, cytosolic (EC 2.7.1.21), TK1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human TK1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Thymidine kinase 1 serves as a critical enzyme in the pyrimidine salvage pathway, phosphorylating thymidine to thymidine monophosphate during DNA synthesis. Its expression is tightly coupled to the cell cycle, peaking during S phase, which makes TK1 an established marker for cellular proliferation. This characteristic has positioned TK1 as a valuable target in cancer research, where elevated serum levels correlate with tumor burden and treatment response across multiple malignancies.

This recombinant monoclonal antibody, generated from clone 6F6, offers the reproducibility that demanding research applications require. Because recombinant technology produces antibodies from defined genetic sequences rather than hybridoma supernatants, you can expect consistent performance across experiments and between lot numbers. This sequence-defined production eliminates the variability that can compromise longitudinal studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your workflow. Western blot analysis confirms specific detection of TK1 at the expected 26 kDa molecular weight across a diverse panel of human cell lines, including 293T, Jurkat, MCF7, A549, PC-3, and K562 cells. This broad validation across epithelial, hematopoietic, and transformed cell types provides confidence when working with various experimental models. Immunofluorescence staining in HeLa cells reveals the cytosolic localization pattern consistent with TK1's known cellular distribution, while flow cytometry analysis demonstrates clear separation between specific signal and isotype control, enabling quantitative assessment of TK1 expression at the single-cell level.

Whether you're investigating cell cycle dynamics, evaluating proliferation markers in cancer models, or exploring nucleotide metabolism, this antibody provides a reliable tool for studying TK1 biology across multiple experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA931459A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate All lanes: Thymidine Kinase 1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa Immunofluorescence staining of Hela with CSB-RA931459A0HU at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 491-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA931459A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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TK1 Recombinant Monoclonal Antibody

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