Code | CSB-RA931459A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The TK1 recombinant monoclonal antibody production generally commences with the insertion of the TK1 antibody-encoding gene into expression vectors. These vectors are subsequently delivered into host cells through polyethyleneimine-mediated transfection. The host cells, housing these vectors, are cultured to generate and release the antibodies. Following purification via affinity chromatography, the antibodies are subjected to assessments through ELISA, WB, IF, and FC tests, confirming their ability to recognize the human TK1 protein.
Thymidine kinase (TK1) is a critical enzyme involved in DNA synthesis and nucleotide salvage pathways. Its primary function is to phosphorylate thymidine, generating essential nucleotide precursors for DNA replication. Beyond its fundamental role in DNA metabolism, thymidine kinase has clinical applications in antiviral and anticancer therapies and serves as a diagnostic marker for certain diseases.
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