Product Details

Uniprot No.

Target Names

Alternative Names

E3 ubiquitin/ISG15 ligase TRIM25 antibody; EFP antibody; Estrogen responsive finger protein antibody; Estrogen-responsive finger protein antibody; RING finger protein 147 antibody; RNF 147 antibody; RNF147 antibody; TRI25 antibody; TRI25_HUMAN antibody; TRIM 25 antibody; Trim25 antibody; Tripartite motif containing 25 antibody; Tripartite motif containing protein 25 antibody; Tripartite motif protein TRIM25 antibody; Tripartite motif-containing protein 25 antibody; Tripartite motifcontaining25 antibody; Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody; Z147 antibody; Zinc finger protein 147 (estrogen responsive finger protein) antibody; Zinc finger protein 147 antibody; Zinc finger protein-147 antibody; ZNF 147 antibody; ZNF147 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human TRIM25

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7E8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TRIM25 serves as a critical E3 ubiquitin and ISG15 ligase that plays essential roles in innate immune signaling, particularly in the RIG-I-mediated antiviral response pathway. Originally identified as an estrogen-responsive finger protein, TRIM25 has emerged as a key regulator of interferon production and has been implicated in various cellular processes including cell proliferation and cancer biology, making it a compelling target for researchers investigating immune regulation, viral defense mechanisms, and oncogenic pathways.

This recombinant monoclonal antibody, clone 7E8, offers the reproducibility and consistency that demanding experimental workflows require. Because it is produced from a defined genetic sequence in rabbit host cells, you can expect uniform performance across different lots, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations. The antibody has been raised against a synthetic peptide derived from human TRIM25 and purified by affinity chromatography to ensure high specificity.

Validation studies demonstrate reliable performance across multiple applications. In western blot analysis of MCF-7 whole cell lysate, the antibody detects a band at the predicted molecular weight of 71 kDa, confirming accurate target recognition in this breast cancer cell line at dilutions between 1:500 and 1:2000. Flow cytometry validation using BxPC-3 pancreatic cancer cells shows clear positive signal separation from isotype control, supporting intracellular detection studies at dilutions of 1:50 to 1:200.

Whether you are investigating TRIM25's role in antiviral immunity, exploring its function in estrogen receptor signaling, or examining its contributions to cancer cell biology, this antibody provides a dependable tool for advancing your research objectives.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks.

Gene References into Functions

formation of unanchored K63-linked poly-ubiquitin chains is unchanged by NS1 binding, indicating that RING dimerisation of TRIM25 is not affected by NS1 PMID: 29739942
The RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain, which we postulate to be a novel RNA-binding domain. PMID: 29117863
TRIM25 inhibits hepatocellular carcinoma progression by targeting MTA1. PMID: 28861931
TRIM25 is a key determinant of breast cancer metastasis. PMID: 28813674
V interaction with TRIM25 and RIG-I prevents TRIM25-mediated ubiquitination of RIG-I and disrupts downstream RIG-I signaling to the mitochondrial antiviral signaling protein. PMID: 29321315
HPV E6 oncoprotein antagonizes the activation of the cytoplasmic innate immune sensor RIG-I by targeting its upstream regulatory enzymes TRIM25 and USP15. We further show that the RIG-I signaling cascade is important for an antiviral innate immune response to HPV16 infection PMID: 29263274
this study shows thatTRIM25 targets ERG for degradation in prostate cancer PMID: 27626314
TRIM25 actively participates in higher-order assembly of the RIG-I signalosome. PMID: 27425606
Our results indicate that TRIM25 is associated with cisplatin resistance and 14-3-3sigma-MDM2-p53 signaling pathway is involved in this process, suggesting targeting TRIM25 may be a potential strategy for the reversal of cisplatin resistance. PMID: 28867193
expression of TRIM25 might be critical for lung cancer cell migration, proliferation as well as doxorubicin resistance. PMID: 26113559
Disturbed p53-MDM2 feedback loop contributing to the pathogenesis of thoracic aortic dissection may be linked to TRIM25 overexpression. PMID: 27903487
the data uncover TRIM25 as a bona fide zinc-finger antiviral protein cofactor that leads to increased zinc-finger antiviral protein modification enhancing its translational inhibition activity. PMID: 28060952
Studies showed that 70% of breast cancer patients were positive for Trim25 expression. PMID: 27528750
we provide a detailed characterization of the TRIM ligases TRIM25 and TRIM32 and show how their oligomeric state is linked to catalytic activity PMID: 27154206
The severe acute respiratory syndrome coronavirus N protein was found to bind to the SPRY domain of the tripartite motif protein 25 (TRIM25) E3 ubiquitin ligase, thereby interfering with the association between TRIM25 and retinoic acid-inducible gene I (RIG-I) and inhibiting TRIM25-mediated RIG-I ubiquitination and activation. PMID: 28148787
TRIM25 is required for Zinc finger antiviral protein optimal binding to target mRNA. PMID: 28202764
TRIM25 may exert its function through TGF-beta pathway. PMID: 26754079
TRIM25 plays an additional role in RIG-I/MDA5 signaling other than RIG-I ubiquitination via activation of NF-kappaB. PMID: 26299329
Data show that TRIM25 enhances p53 and Mdm2 abundance by preventing their proteasomal degradation. At the same time, TRIM25 inhibits p53's transcriptional activity and dampens the response to DNA damage. PMID: 25728675
The PR-2B DENV-2 produced increased levels of subgenomic flavivirus RNA (sfRNA) relative to genomic RNA during replication. PR-2B sfRNA showed sequence dependent binding to and prevention of tripartite motif 25 (TRIM25) deubiquitylation, which is critical for sustained and amplified retinoic acid-inducible gene 1 (RIG-I)-induced type I interferon expression. PMID: 26138103
Trim25 binds to the conserved terminal loop (CTL) of pre-let-7 and activates TuT4, allowing for more efficient Lin28a-mediated uridylation. PMID: 25457611
Our data uncovers a previously unknown functional link between gp78 and TRIM25 and provides mechanistic insight into gp78-mediated protein ubiquitylation. PMID: 24810856
Data indicate ubiquitin-specific protease 15 (USP15) as a critical regulator of the tripartite motif protein 25 (TRIM25)- and RNA sensor retinoic acid-inducible gene-I (RIG-I)-mediated antiviral immune response PMID: 24399297
In controls, RNF125 is the highest expressed gene, whereas in HIV infection progressors, RIG-I is either the highest expressed gene or is expressed similarly to RNF125 and TRIM25. PMID: 24131985
Study reports that RLR activation triggers MAVS ubiquitination on lysine 7 and 10 by the E3 ubiquitin ligase TRIM25 and marks it for proteasomal degradation concomitantly with downstream signaling. PMID: 22626058
EFP mediates estrogen-induced ATBF1 protein degradation in breast cancer cells. PMID: 22452784
Studies indicate that the splice variant of RIG-I encodes a protein that lacks the first CARD of RIG-I, and the variant RIG-I protein is not ubiquitinated by TRIM25. PMID: 21890623
RING-IBR-RING E3 ligase domains of HOIL-1L and HOIP bind and induce proteasomal degradation of TRIM25, whereas the NZF domain of HOIL-1L competes with TRIM25 for RIG-I binding PMID: 21292167
Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. PMID: 21266548
The regulation of Efp and bFGF expression during endometrial carcinogenesis suggests their potential utility as a prognostic biomarker. PMID: 20127022
Efp controls breast cancer growth through ubiquitin-dependent proteolysis of 14-3-3sigma. PMID: 12943693
Results indicate that estrogen-responsive finger protein is widely expressed and may play important roles in various human tissues, possibly through estrogen receptors. PMID: 15130519
Over expression of Estrogen-responsive finger protein is associated with tumor progression of breast carcinoma PMID: 16144914
14-3-3 sigma expression inversely correlated with estrogen receptor alpha, progesterone receptor and estrogen-responsive finger protein, and positively correlated with myometrial invasion and lymph node metastasis in endometrial adenocarcinoma PMID: 16271083
Efp is an IFN-responsive gene that potentially mediates IFN actions, involved in ISGylation and ubiquitination of proteins including Efp itself. PMID: 17069755
Our results indicate that EFP functions as a cofactor for ERalpha-mediated transcription. PMID: 17418098
role of the intact tandem CARD for TRIM25-mediated RIG-I activation; TRIM25 binds the RIG-I first CARD and subsequently ubiquitinates its second CARD PMID: 18948594
estradiol and tamoxifen may regulate the growth of endometrial carcinoma cells by stimulating VEGF production through Efp. PMID: 19148513
Viral nonstructural protein 1 inhibits activation of RIG-I by binding to TRIM25. PMID: 19454344
NS1 inhibits TRIM25-mediated RIG-I ubiquitination, thereby suppressing RIG-I signal transduction. PMID: 19454348

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Subcellular Location

Cytoplasm. Cytoplasm, Stress granule. Nucleus.

Tissue Specificity

Expressed in breast tumors (at protein level). Ubiquitous.

Database Links

HGNC: 12932

OMIM: 600453

KEGG: hsa:7706

STRING: 9606.ENSP00000323889

UniGene: Hs.528952

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Code	CSB-RA090616A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate(30µg) All lanes: TRIM25 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time：20s Overlay Peak curve showing BxPC-3 cells stained with CSB-RA090616A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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TRIM25 Recombinant Monoclonal Antibody

Product Details

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Target Background