TRMT2A Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA223383A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:MCF7 whole cell lysate(30µg), Hela whole cell lysate(30µg) All lanes: TRMT2A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time：60s IHC image of CSB-RA223383A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA223383A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HeLa cell with CSB-RA223383A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Uniprot No.

Q8IZ69

Target Names

TRMT2A

Alternative Names

HpaII tiny fragments locus 9c protein antibody; HTF9C antibody; TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody; TRM2 tRNA methyltransferase 2 homolog A antibody; TRM2A_HUMAN antibody; TRMT2A antibody; tRNA (uracil-5-)-methyltransferase homolog A antibody; tRNA methyltransferase 2 homolog A antibody; tRNA methyltransferase 2, S. cerevisiae, homolog of, A antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human TRMT2A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5B1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

May be involved in nucleic acid metabolism and/or modifications.

Gene References into Functions

TRMT2A staining showed a strong correlation with likelihood of recurrence at five years in 67 HER2+ breast cancer. PMID: 20307320
Single nucleotid polymorphisms of this gene may be associated with a deficit in sustained attention within schizophrenia, in a Taiwanese cohort. PMID: 18075473

Protein Families

Class I-like SAM-binding methyltransferase superfamily, RNA M5U methyltransferase family

Database Links

HGNC: 24974

OMIM: 611151

KEGG: hsa:27037

STRING: 9606.ENSP00000252136

UniGene: Hs.713579

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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