Code | CSB-RA186773A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
The production process of the TRPM8 recombinant monoclonal antibody began with the synthesis of the gene encoding the TRPM8 monoclonal antibody. Immunizing animals using a synthesized peptide derived from human TRPM8 and isolating B cells from the immunized animals. B cells were fused with myeloma cells to generate hybridomas, which were screened for TRPM8 antibody production. The variable light and variable heavy domains of the TRPM8 antibody were then sequenced, and the TRPM8 monoclonal antibody gene was cloned into a vector. The recombinant vector was transfected into cells for cultivation, and the resulting TRPM8 recombinant monoclonal antibody was purified through affinity chromatography from the cell culture supernatant. This highly specific TRPM8 recombinant monoclonal antibody can react with both human and mouse TRPM8 samples and has been tested in ELISA and IHC applications.
The TRPM8 protein is a cation channel that is mainly expressed in sensory neurons. It is activated by cold temperatures and menthol and plays a role in thermosensation and nociception. When activated, TRPM8 allows the influx of calcium and sodium ions into the cell, leading to the depolarization of the neuron and the initiation of nerve impulses. In addition to its role in thermosensation and nociception, TRPM8 has also been implicated in various physiological processes, such as inflammation, cancer, and bladder function.
There are currently no reviews for this product.