TXNDC12 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA995859A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), K562 whole cell lysate(30µg), Rat liver tissue lysate(30µg) All lanes:TXNDC12 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time：3min IHC image of CSB-RA995859A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing HepG2 cells stained with CSB-RA995859A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

O95881

Target Names

TXNDC12

Alternative Names

AG1 antibody; AGR1 antibody; anterior gradient homolog 1 antibody; endoplasmic reticulum protein ERp19 antibody; Endoplasmic reticulum resident protein 18 antibody; Endoplasmic reticulum resident protein 19 antibody; endoplasmic reticulum thioredoxin superfamily member, 18 kDa antibody; ER protein 18 antibody; ER protein 19 antibody; ERP 18 antibody; ERP16 antibody; ERp18 antibody; ERp19 antibody; hAG 1 antibody; hAG1 antibody; hTLP19 antibody; PDIA16 antibody; protein disulfide isomerase family A, member 16 antibody; thioredoxin domain containing 12 (endoplasmic reticulum antibody; Thioredoxin domain-containing protein 12 antibody; thioredoxin like protein p19 antibody; Thioredoxin-like protein p19 antibody; TLP19 antibody; TXD12_HUMAN antibody; TXNDC12 antibody

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide from human TXNDC12 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21A7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Possesses significant protein thiol-disulfide oxidase activity.

Gene References into Functions

We conclude that ERp19 contributes to tumorigenicity and metastasis of gastric cancer PMID: 25940440
ERp18 shows specificity towards a component of the complement cascade, pentraxin-related protein PTX3. PMID: 19887585
putative physiological role for endoplasmic reticulum thioredoxin superfamily member p18(ERp18) in native disulfide bond formation is discussed PMID: 12761212
ERp16 mediates disulfide bond formation in the ER and plays an important role in cellular defense against prolonged ER stress PMID: 18628206
the solution structure of oxidized ERp18 as determined using NMR spectroscopy PMID: 19361226

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Subcellular Location

Endoplasmic reticulum lumen.

Tissue Specificity

Widely expressed.

Database Links

HGNC: 24626

OMIM: 609448

KEGG: hsa:51060

STRING: 9606.ENSP00000360688

UniGene: Hs.476033

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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