Product Details

Uniprot No.

Target Names

Alternative Names

Tyrosine-protein kinase receptor TYRO3 (EC 2.7.10.1) (Tyrosine-protein kinase BYK) (Tyrosine-protein kinase DTK) (Tyrosine-protein kinase RSE) (Tyrosine-protein kinase SKY) (Tyrosine-protein kinase TIF), TYRO3, BYK DTK RSE SKY TIF

Species Reactivity

Human

Immunogen

A synthesized peptide from human TYRO3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8D8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TYRO3 is a receptor tyrosine kinase belonging to the TAM family, playing essential roles in cell survival, proliferation, and the regulation of innate immunity. This kinase participates in the clearance of apoptotic cells and has emerged as a significant focus in cancer research, where its overexpression has been linked to tumor progression and therapeutic resistance across multiple malignancies.

This recombinant monoclonal antibody, clone 8D8, offers researchers the reproducibility and consistency that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format provides excellent sensitivity while the affinity-chromatography purification delivers a clean reagent suitable for demanding applications.

Validation studies confirm reliable performance in Western blot and immunohistochemistry workflows. In Western blot analysis of Jurkat whole cell lysate, the antibody detects TYRO3 at an observed molecular weight of approximately 128 kDa, which is higher than the predicted 97 kDa. This size difference is consistent with extensive post-translational glycosylation characteristic of receptor tyrosine kinases. Immunohistochemical staining in paraffin-embedded human cervical cancer tissue demonstrates clear visualization of TYRO3 expression, with optimal results achieved using citrate buffer antigen retrieval and overnight primary antibody incubation.

The antibody's validated performance across both biochemical and tissue-based applications makes it well-suited for researchers investigating TAM receptor biology, tumor microenvironment interactions, or therapeutic target validation in oncology programs.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.; (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.; (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

Gene References into Functions

Data suggest that TYRO3-mediated phosphorylation of ACTN4 is involved in invasiveness of melanoma cells; TYRO3-mediated phosphorylation of ACTN4 requires FAK activation at tyrosine 397. (TYRO3 = TYRO3 protein tyrosine kinase; ACTN4 = actinin alpha 4; FAK = focal adhesion kinase isoform FAK1) PMID: 29274473
Phosphatidylserine mediated hyperactivation of TYRO3.TYRO3 promotes epithelial cell efferocytosis in a tyrosine kinase-dependent manner.TYRO3 role in AKT-dependent drug resistance. PMID: 28184013
Taken together, our data indicate that elevated plasma Gas6 levels is associated with the severity of disease during HTNV infection in humans, suggesting that Gas6 may play an important role by binding with Tyro3 on monocytes. PMID: 28537534
Study identified the Gas6/TAM receptor pathway with Tyro3 and Mer as novel targets in colorectal cancer. PMID: 27486820
These results show that TYRO3, AXL and GAS6 are expressed at higher levels in LMS and expression of its ligands correlates to a worse PFS in LMS patients. PMID: 29024938
Patients with macroalbuminuria diabetes had higher circulating levels of sMer and more urinary soluble Tyro3 and sMer than normoalbuminuric diabetics. Increased clearance of sTyro3 and sMer was associated with loss of tubular Tyro3 and Mer expression in diabetic nephropathy tissue. During in vitro diabetes, human kidney cells had down-regulation of Tyro3 and Mer mRNA and increased shedding of sTyro3 and sMer. PMID: 28668213
TYRO3 is overexpressed in the early stage of colon cancer development and aberrant expression of TYRO3 promotes tumorigenesis and induces EMT through the regulation of SNAI1. PMID: 27132510
In this paper, we review the biology of the Gas6/Tyro3, Axl, and MerTK(collectively named TAM system)and the current evidence supporting its potential role in the pathogenesis of multiple sclerosis . PMID: 27801848
these data suggest that Tyro3 contributes significantly to tumor growth, aggressiveness and liver dysfunction PMID: 26573872
Tyro3 gene dosage modulates Mertk-associated retinal degeneration, provide strong evidence for a direct role for TYRO3 in RPE phagocytosis, and suggest that an eQTL can modify a recessive Inherited photoreceptor degenerations. PMID: 26656104
The mRNA expression levels of Tyro-3, Axl were decreased in pSS patients. When considering the plasma level, increased levels of soluble Mer was observed with statistically significant difference. PMID: 25881761
genetic ablation of a receptor tyrosine kinase encoded byTyro3in mice or the functional neutralization of its ortholog in human dendritic cells resulted in enhanced type 2 immunity. PMID: 27034374
the results of the present study demonstrated that the acquired taxol resistance of ovarian cancer cells was associated with ROS-dependent upregulation in the expression of Tyro3 RTK and the subsequent activation of Akt. PMID: 25815442
Significantly increased levels of sMer, sTyro3 and sAxl may be important factors contributing to the deficit in phagocytosis ability in systemic lupus erythematosus . PMID: 25878564
Tetherin phosphorylation induces the recruitment of Syk which is required for downstream NF-kappaB activation. PMID: 25211072
These studies demonstrate that, despite their similarity, TYRO3, AXL, and MER are likely to perform distinct functions in both immunoregulation and the recognition and removal of ACs. PMID: 25074926
Gas6, a ligand of Tyro3, exerts an autocrine activities though Tyro3 and Axl in a subgroup of leiomyosarcoma. PMID: 23354874
The effects of TYRO3-knockdown by small interfering RNA (siRNA) on proliferation, cell-cycle distribution, and cell signaling in different breast neoplasm cell lines were compared. PMID: 24982338
These findings indicate that Tyro3 is a critical signal for synovial hyperplasia, osteoclast differentiation and bone erosion during arthritis. PMID: 23632195
[review] Receptor tyrosine kinases Tyro-3, Axl and Mer, collectively designated as TAM, are involved in the clearance of apoptotic cells. PMID: 23662598
Low expression of the Syk gene may play an important role in tumorigenesis in esophageal cancer. PMID: 23865365
data indicate that Tyro3 may confer increased survival signals in melanoma cells and can be stymied using inhibitory mAbs PMID: 23570341
analysis of novel and selective spiroindoline-based inhibitors of Sky kinase PMID: 22119469
Axl,Tyro3,DC-SIGN and LSECtin are identified as new virus receptors for Lassa virus cell entry. PMID: 22156524
plasma concentrations of sMer and sTyro3 were significantly increased in patients with active systemic lupus erythematosis and Rheumatoid arthritis PMID: 21496228
expression is downregulated in atherosclerotic carotid plaque PMID: 21384080
Our findings suggest that a TYRO3/AXL-GAS6 autocrine circuit sustains the malignant features of thyroid cancer cells PMID: 21343401
Gas6 and Tyro3, Axl and Mer (TAM) receptors have roles in human and murine platelet activation and thrombus stabilization PMID: 20546121
correlation of decreased protein S levels with lupus disease activity is consistent with a role for the TAM receptors in scavenging apoptotic cells and controlling inflammation PMID: 20637106
Findings indicate genetic association between MERTK and TYRO3 allelic variants and carotid atherosclerosis. PMID: 20664904
Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. PMID: 20348395
Results identified the receptor protein tyrosine kinase TYRO3 as an upstream regulator of MITF expression by a genome-wide gain-of-function cDNA screen. PMID: 19805117
Gas6 receptors were not upregulated in any of the allograft groups, except for the Axl receptor, which increased only in acute tubular necrosis. PMID: 12768229
Tyro3 may have roles in signal transduction and cell adhesion PMID: 14623883
Axl, Sky and Mer are Gas6 receptors that enhance platelet activation and regulate thrombotic responses PMID: 15733062
Results identify and characterise a novel interaction between RanBPM and the related receptor tyrosine kinases, Axl and Sky. PMID: 15964779
Here we show the involvement of members of the Tyro3 receptor tyrosine kinase family-Axl, Dtk, and Mer-in cell entry of filoviruses. PMID: 17005688
Possible strategies for targeted inhibition of the TAM family in the treatment of human cancer. PMID: 18620092

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, AXL/UFO subfamily

Tissue Specificity

Abundant in the brain and lower levels in other tissues.

Database Links

HGNC: 12446

OMIM: 600341

KEGG: hsa:7301

STRING: 9606.ENSP00000263798

UniGene: Hs.381282

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Code	CSB-RA795810A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg) All lanes: TYRO3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 97 kDa Observed band size: 128 kDa Exposure time：3min IHC image of CSB-RA795810A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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TYRO3 Recombinant Monoclonal Antibody

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