UBA1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA105164A0HU
Size	US$210
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Image	IHC image of CSB-RA105164A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of PC-3 cell with CSB-RA105164A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing K562 cells stained with CSB-RA105164A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P22314

Target Names

UBA1

Alternative Names

Ubiquitin-like modifier-activating enzyme 1 (EC 6.2.1.45) (Protein A1S9) (Ubiquitin-activating enzyme E1), UBA1, A1S9T UBE1

Species Reactivity

Human

Immunogen

A synthesized peptide from human UBA1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

UBA1, also known as ubiquitin-activating enzyme E1, serves as the essential first enzyme in the ubiquitin-proteasome pathway, catalyzing the ATP-dependent activation of ubiquitin that initiates protein degradation and cellular quality control mechanisms. As the primary E1 enzyme in human cells, UBA1 plays a fundamental role in regulating protein turnover, cell cycle progression, and DNA damage responses, making it a critical target for researchers investigating proteostasis, cancer biology, and neurodegenerative disease mechanisms.

This recombinant monoclonal antibody, clone 8C10, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a sequence-defined rabbit IgG format, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The defined molecular composition ensures that your results remain comparable across experiments, whether you're establishing baseline data or building on previous findings.

Validation across multiple detection platforms demonstrates this antibody's versatility in your research toolkit. Immunohistochemistry studies in paraffin-embedded human kidney tissue using a Leica Bond system with citrate buffer antigen retrieval show clear target detection at 1:100 dilution. Immunofluorescence analysis in PC-3 prostate cancer cells reveals distinct staining patterns when counter-stained with DAPI, while flow cytometry experiments with K562 cells demonstrate a clear positive shift compared to isotype controls, confirming specific detection in suspension cell applications.

Whether you're investigating ubiquitin pathway dysregulation in malignancy, exploring therapeutic targets in protein homeostasis disorders, or characterizing E1 enzyme expression patterns across tissue types, this antibody provides the reliable detection performance needed to advance your understanding of this fundamental cellular machinery.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.

Gene References into Functions

Polyubiquitination and proteasomal degradation of ezrin and CUGBP1 require Uba6, but not Uba1, and that Uba6 is involved in the control of ezrin localization and epithelial morphogenesis. These data suggest that distinctive substrate pools exist for Uba1 and Uba6 that reflect non-redundant biological roles for Uba6. PMID: 28134249
Results from a study on gene expression variability markers in early-stage human embryos shows that UBA1 is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
recent findings put UBA1 at the center of cellular homeostasis and neurodegeneration [review] PMID: 26432019
A ubiquitin shuttle DC-UbP reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes. PMID: 25207809
Conjugation of the ubiquitin activating enzyme UBE1 with the ubiquitin-like modifier FAT10 targets it for proteasomal degradation PMID: 25768649
Ubiquitin-activating enzyme is necessary for 17beta-estradiol-induced breast cancer cell proliferation and migration. PMID: 25138535
We expressed and purified the N-terminal domains of human E1 and determined their crystal structures, which contain inactive adenylation domain (IAD) and the first catalytic cysteine half-domain (FCCH) PMID: 25209502
Diminished ubiquitylation phenotype observed in enteropathogenic Escherichia coli infected cells corresponds to a strong reduction in the abundance of both Ube1 and Uba6. PMID: 22999844
Decrease in free ubiquitin levels under stress allows NEDD8 to be conjugated through Ube1. PMID: 22370482
UBA1 knockdown impairs formation of ubiquitin conjugates at DNA double-strand breaks. PMID: 22456334
Data show that largazole and ester/keto analogs inhibit ubiquitin E1 activation. PMID: 22279528
In conclusion, nUBE1L is a novel human E1 like gene highly expressed in adult testis, which plays key role in ubiquitin system, and accordingly influences spermatogenesis and male fertility. PMID: 15202508
analysis of ATP.Mg2+ binding in the catalytic cycle of ubiquitin-activating enzyme PMID: 16595681
unusual type of regulation at the level of the E1 enzyme is likely to affect numerous cellular processes and may represent a strategy to coordinate multiple phenotypic changes upon differentiation by using E1 as a "master switch." PMID: 17060614
Human Uba6 and Ube1 have distinct preferences for E2 charging in vitro, and their specificity depends in part on their C-terminal ubiquitin-fold domains, which recruit E2s PMID: 17597759
Large-scale mutation analysis in genes resulted in detection of 3 rare novel variants in exon 15 of UBE1 that segregate with X-linked infantile spinal muscular atrophy. PMID: 18179898
high-level expression of UBE1 was seen inextranodal NK/T cell lymphoma, nasal type; there was no significant difference between UBE1 expression and patients' outcome PMID: 18661401

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Involvement in disease

Spinal muscular atrophy X-linked 2 (SMAX2)

Subcellular Location

Cytoplasm. Mitochondrion. Nucleus.; [Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm.

Protein Families

Ubiquitin-activating E1 family

Tissue Specificity

Detected in erythrocytes (at protein level). Ubiquitous.

Database Links

HGNC: 12469

OMIM: 301830

KEGG: hsa:7317

STRING: 9606.ENSP00000338413

UniGene: Hs.533273

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Ubiquitin mediated proteolysis

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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