Name: UBA1 Antibody
Brand: CUSABIO
SKU: CSB-PA00149A0Rb

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) UBA1 Polyclonal antibody

Uniprot No.

P22314

Target Names

UBA1

Alternative Names

A1S9 antibody; A1S9 protein antibody; A1S9T and BN75 temperature sensitivity complementing antibody; A1S9T antibody; A1ST antibody; AMCX1 antibody; CFAP124 antibody; CTD-2522E6.1 antibody; GXP 1 antibody; GXP1 antibody; MGC4781 antibody; POC20 antibody; POC20 centriolar protein homolog antibody; Protein A1S9 antibody; SMAX2 antibody; Uba1 antibody; UBA1, ubiquitin-activating enzyme E1 homolog A antibody; UBA1_HUMAN antibody; UBA1A antibody; UBE 1 antibody; UBE 1X antibody; UBE1 antibody; UBE1X antibody; Ubiquitin activating enzyme E1 antibody; Ubiquitin-activating enzyme E1 antibody; Ubiquitin-like modifier-activating enzyme 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ubiquitin-like modifier-activating enzyme 1 protein (730-881AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The UBA1 Antibody (Product code: CSB-PA00149A0Rb) is Non-conjugated. For UBA1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA00149B0Rb	UBA1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA00149C0Rb	UBA1 Antibody, FITC conjugated
Biotin	CSB-PA00149D0Rb	UBA1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function	Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
Gene References into Functions	Polyubiquitination and proteasomal degradation of ezrin and CUGBP1 require Uba6, but not Uba1, and that Uba6 is involved in the control of ezrin localization and epithelial morphogenesis. These data suggest that distinctive substrate pools exist for Uba1 and Uba6 that reflect non-redundant biological roles for Uba6. PMID: 28134249 Results from a study on gene expression variability markers in early-stage human embryos shows that UBA1 is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249 recent findings put UBA1 at the center of cellular homeostasis and neurodegeneration [review] PMID: 26432019 A ubiquitin shuttle DC-UbP reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes. PMID: 25207809 Conjugation of the ubiquitin activating enzyme UBE1 with the ubiquitin-like modifier FAT10 targets it for proteasomal degradation PMID: 25768649 Ubiquitin-activating enzyme is necessary for 17beta-estradiol-induced breast cancer cell proliferation and migration. PMID: 25138535 We expressed and purified the N-terminal domains of human E1 and determined their crystal structures, which contain inactive adenylation domain (IAD) and the first catalytic cysteine half-domain (FCCH) PMID: 25209502 Diminished ubiquitylation phenotype observed in enteropathogenic Escherichia coli infected cells corresponds to a strong reduction in the abundance of both Ube1 and Uba6. PMID: 22999844 Decrease in free ubiquitin levels under stress allows NEDD8 to be conjugated through Ube1. PMID: 22370482 UBA1 knockdown impairs formation of ubiquitin conjugates at DNA double-strand breaks. PMID: 22456334 Data show that largazole and ester/keto analogs inhibit ubiquitin E1 activation. PMID: 22279528 In conclusion, nUBE1L is a novel human E1 like gene highly expressed in adult testis, which plays key role in ubiquitin system, and accordingly influences spermatogenesis and male fertility. PMID: 15202508 analysis of ATP.Mg2+ binding in the catalytic cycle of ubiquitin-activating enzyme PMID: 16595681 unusual type of regulation at the level of the E1 enzyme is likely to affect numerous cellular processes and may represent a strategy to coordinate multiple phenotypic changes upon differentiation by using E1 as a "master switch." PMID: 17060614 Human Uba6 and Ube1 have distinct preferences for E2 charging in vitro, and their specificity depends in part on their C-terminal ubiquitin-fold domains, which recruit E2s PMID: 17597759 Large-scale mutation analysis in genes resulted in detection of 3 rare novel variants in exon 15 of UBE1 that segregate with X-linked infantile spinal muscular atrophy. PMID: 18179898 high-level expression of UBE1 was seen inextranodal NK/T cell lymphoma, nasal type; there was no significant difference between UBE1 expression and patients' outcome PMID: 18661401 Show More Hide All
Involvement in disease	Spinal muscular atrophy X-linked 2 (SMAX2)
Subcellular Location	Cytoplasm. Mitochondrion. Nucleus.; [Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm.
Protein Families	Ubiquitin-activating E1 family
Tissue Specificity	Detected in erythrocytes (at protein level). Ubiquitous.
Database Links	HGNC: 12469 OMIM: 301830 KEGG: hsa:7317 STRING: 9606.ENSP00000338413 UniGene: Hs.533273

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Code	CSB-PA00149A0Rb
Size	US$299Purchase it in Cusabio online store (only available for customers from the US)
Image	Western Blot Positive WB detected in: Hela whole cell lysate All lanes: UBA1 antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 118, 114 kDa Observed band size: 118, 114 kDa Immunofluorescence staining of Hela cells with CSB-PA00149A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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UBA1 Antibody

Product Details

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Target Background

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