UBA1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA821297A0HU
Size	US$210
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Image	Western Blot Positive WB detected in:HEK293 whole cell lysate(30µg) All lanes: UBA1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 118 kDa Observed band size: 118 kDa Exposure time：1min IHC image of CSB-RA821297A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA821297A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Uniprot No.

P22314

Target Names

UBA1

Alternative Names

Ubiquitin-like modifier-activating enzyme 1 (EC 6.2.1.45) (Protein A1S9) (Ubiquitin-activating enzyme E1), UBA1, A1S9T UBE1

Species Reactivity

Human

Immunogen

A synthesized peptide from human UBA1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8A10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

UBA1, or ubiquitin-like modifier-activating enzyme 1, serves as the essential first enzyme in the ubiquitin-proteasome pathway, catalyzing the ATP-dependent activation of ubiquitin that initiates protein degradation and cellular quality control. As the primary E1 enzyme in human cells, UBA1 plays a fundamental role in proteostasis, cell cycle regulation, and DNA damage response, making it a critical target for researchers investigating cancer biology, neurodegenerative diseases, and emerging therapeutic strategies targeting the ubiquitin system.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human UBA1, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, you can expect uniform performance across lots, eliminating the variability that can complicate long-term studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis in HEK293 whole cell lysates detects a clean band at the predicted 118 kDa molecular weight, confirming specific target recognition at dilutions between 1:500 and 1:2000. For tissue-based studies, immunohistochemistry in paraffin-embedded human kidney sections reveals clear staining patterns, while immunofluorescence in HeLa cells enables subcellular localization studies with robust signal quality.

The unconjugated format provides flexibility for pairing with your preferred detection systems, and the glycerol-stabilized formulation supports long-term storage stability at -20°C or -80°C. Whether you're characterizing UBA1 expression changes in disease models, validating ubiquitination pathway components, or exploring E1 enzyme biology in cellular contexts, this antibody delivers the specificity and application range to support your investigations.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.

Gene References into Functions

Polyubiquitination and proteasomal degradation of ezrin and CUGBP1 require Uba6, but not Uba1, and that Uba6 is involved in the control of ezrin localization and epithelial morphogenesis. These data suggest that distinctive substrate pools exist for Uba1 and Uba6 that reflect non-redundant biological roles for Uba6. PMID: 28134249
Results from a study on gene expression variability markers in early-stage human embryos shows that UBA1 is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
recent findings put UBA1 at the center of cellular homeostasis and neurodegeneration [review] PMID: 26432019
A ubiquitin shuttle DC-UbP reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes. PMID: 25207809
Conjugation of the ubiquitin activating enzyme UBE1 with the ubiquitin-like modifier FAT10 targets it for proteasomal degradation PMID: 25768649
Ubiquitin-activating enzyme is necessary for 17beta-estradiol-induced breast cancer cell proliferation and migration. PMID: 25138535
We expressed and purified the N-terminal domains of human E1 and determined their crystal structures, which contain inactive adenylation domain (IAD) and the first catalytic cysteine half-domain (FCCH) PMID: 25209502
Diminished ubiquitylation phenotype observed in enteropathogenic Escherichia coli infected cells corresponds to a strong reduction in the abundance of both Ube1 and Uba6. PMID: 22999844
Decrease in free ubiquitin levels under stress allows NEDD8 to be conjugated through Ube1. PMID: 22370482
UBA1 knockdown impairs formation of ubiquitin conjugates at DNA double-strand breaks. PMID: 22456334
Data show that largazole and ester/keto analogs inhibit ubiquitin E1 activation. PMID: 22279528
In conclusion, nUBE1L is a novel human E1 like gene highly expressed in adult testis, which plays key role in ubiquitin system, and accordingly influences spermatogenesis and male fertility. PMID: 15202508
analysis of ATP.Mg2+ binding in the catalytic cycle of ubiquitin-activating enzyme PMID: 16595681
unusual type of regulation at the level of the E1 enzyme is likely to affect numerous cellular processes and may represent a strategy to coordinate multiple phenotypic changes upon differentiation by using E1 as a "master switch." PMID: 17060614
Human Uba6 and Ube1 have distinct preferences for E2 charging in vitro, and their specificity depends in part on their C-terminal ubiquitin-fold domains, which recruit E2s PMID: 17597759
Large-scale mutation analysis in genes resulted in detection of 3 rare novel variants in exon 15 of UBE1 that segregate with X-linked infantile spinal muscular atrophy. PMID: 18179898
high-level expression of UBE1 was seen inextranodal NK/T cell lymphoma, nasal type; there was no significant difference between UBE1 expression and patients' outcome PMID: 18661401

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Involvement in disease

Spinal muscular atrophy X-linked 2 (SMAX2)

Subcellular Location

Cytoplasm. Mitochondrion. Nucleus.; [Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm.

Protein Families

Ubiquitin-activating E1 family

Tissue Specificity

Detected in erythrocytes (at protein level). Ubiquitous.

Database Links

HGNC: 12469

OMIM: 301830

KEGG: hsa:7317

STRING: 9606.ENSP00000338413

UniGene: Hs.533273

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Related pathways

Ubiquitin mediated proteolysis

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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