Product Details

Uniprot No.

P0CG47

Target Names

UBB

Alternative Names

Polyubiquitin-B [Cleaved into: Ubiquitin], UBB

Species Reactivity

Human

Immunogen

A synthesized peptide from human UBB protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

18C12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Ubiquitin B represents one of the most evolutionarily conserved proteins across eukaryotes, serving as the fundamental building block of the ubiquitin-proteasome system. This polyubiquitin precursor is cleaved to generate functional ubiquitin monomers that orchestrate protein degradation, cell cycle regulation, DNA repair, and signal transduction pathways. Dysregulation of ubiquitin homeostasis has been implicated in neurodegenerative diseases, cancer progression, and immune dysfunction, making UBB a compelling target for researchers investigating proteostasis and cellular stress responses.

This recombinant monoclonal antibody, clone 18C12, offers the reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human UBB, the antibody is produced using recombinant technology, ensuring sequence-defined consistency between lots. This eliminates the variability inherent in traditional hybridoma-derived antibodies, allowing researchers to confidently compare results across extended studies and collaborative projects.

Validation studies demonstrate robust performance in immunofluorescence and flow cytometry applications. Immunofluorescence staining of MCF-7 breast cancer cells reveals clear signal with recommended dilutions of 1:50–1:200, while flow cytometric analysis of HeLa cells shows distinct positive population shifts compared to isotype controls. These validated human cell lines provide researchers with established starting points for their own experimental optimization.

The antibody is supplied in a stabilized liquid format containing glycerol for long-term storage stability at -20°C or -80°C. Affinity purification ensures high specificity for downstream applications. Whether investigating ubiquitin dynamics in cancer biology, exploring protein quality control mechanisms, or characterizing cellular stress responses, this antibody provides a reliable tool for visualizing and quantifying UBB expression at the single-cell level.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Gene References into Functions

This study demonstrated that the Deposition of mutant ubiquitin in parkinsonism-dementia complex of Guam. PMID: 29122008
The polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB have been characterized. PMID: 27861798
The C-terminal five residues of Ub, RLRGG, are responsible for the interaction with the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease. PMID: 27245450
A new crystallographic structure of human ubiquitin solved from crystals grown in the presence of magnesium. PMID: 26750481
Data suggest that both human ubiquitin and HFBII (hydrophobin-II from Trichoderma reesei) exhibit a critical surface hydration level (or effective hydrophobic interface at the surface) at which percolation transition of water network occurs. PMID: 25204743
UbB was significantly increased in prolonged Trichostatin A-selected HeLa cells and it played a key role in the maintenance of cervical cancer stem-like cells PMID: 24367661
downregulation of ubiquitin through Ubb-KD is a potential anti-cancer treatment by inhibiting ubiquitination at multiple sites related to oncogenic pathways and by weakening the ability of cancer cells to overcome increased stress. PMID: 24022007
A significant decrease in amyloid beta deposition and plaque formation suggests a role for the ubiquitin-proteasome system in the amyloid pathology of Alzheimer's disease. PMID: 22797007
age-dependent accumulation of Ubb(+1) , and how Ubb(+1) -mediated proteasome inhibition may contribute to Alzheimer's disease. [review] PMID: 22082077
Studies indicate that biomedical research on ubiquitin moves into translational research and drug discovery. PMID: 21544573
Studies indicate that DUBs recycle ubiquitin by processing polyubiquitin chains to generate free ubiquitin, and can be regulated by ubiquitination or phosphorylation. PMID: 21480003
analysis of orexin receptor 1 and 2 -arrestin-ubiquitin complexes PMID: 21378163
The results of this study demonstrated that the UBB mutation caused the subtle defect in spatial reference memory formation, caused by a decrease in forebrain proteasome activity. PMID: 21059367
Studies indicate that indicating that the inhibition of the ubiquitin-proteasome system could be used as a novel approach for cancer therapy. PMID: 20491623
Results suggest that the interaction between E2-25K and UBB(+1) is critical for the synthesis and accumulation of UBB(+1)-anchored polyubiquitin, which results in proteasomal inhibition and neuronal cell death. PMID: 20826778
Molecular misreading of the ubiquitin B gene and hepatic mallory body formation. PMID: 12055595
expression of UBB+1 causes proteasome inhibition and induces expression of heat-shock proteins; although UBB+1-expressing cells have a compromised ubiquitin-proteasome system, they are protected against oxidative stress PMID: 12871580
The temporal localization of frame-shift ubiquitin-B and amyloid precursor protein, and complement proteins in the brain of non-demented control patients with increasing Alzheimer's disease pathology. PMID: 12893422
In sporadic inclusion-body myositis, UBB+1 may be pathogenic by inhibiting proteasome, thereby promoting accumulation of cytotoxic misfolded amyloid-beta and phosphorylated-tau. PMID: 15452314
UBB+1, a mutant form of ubiquitin was present in the majority of NFTs, whereas co-existence of alpha-synuclein and UBB+1 was found in only a few neurons in cases of combined multiple system atrophy and Alzheimer's disease. PMID: 17237936
K63-polyubiquitination guards against chemical carcinogenesis by preventing mutagenesis and thus contributing to genomic stability PMID: 17395554
UBB(+1), at low expression levels, is efficiently degraded by the proteasome, but at high levels, the proteasome failed to degrade UBB(+1), causing its accumulation. PMID: 17405812
analyse the expression of mutant ubiquitin (UBB+1), in muscle biopsies from patients suffering from myotilinopathy and desminopathy PMID: 17931355
The expression a mutant human UBB analogous (UB14) in yeast markedly enhanced cellular susceptibility to toxic protein aggregates. PMID: 19214209

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Subcellular Location

[Ubiquitin]: Cytoplasm. Nucleus.

Protein Families

Ubiquitin family

Database Links

HGNC: 12463

OMIM: 191339

KEGG: hsa:7314

STRING: 9606.ENSP00000304697

UniGene: Hs.356190

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Code	CSB-RA036685A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of MCF-7 cell with CSB-RA036685A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA036685A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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UBB Recombinant Monoclonal Antibody

Product Details

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Target Background