Product Details

Uniprot No.

Target Names

Alternative Names

Cyclin selective ubiquitin carrier protein antibody; dJ447F3.2 antibody; Mitotic specific ubiquitin conjugating enzyme antibody; UB E2C antibody; UBCH 10 antibody; UbcH10 antibody; UBE 2C antibody; Ube2c antibody; UBE2C_HUMAN antibody; Ubiquitin carrier protein C antibody; Ubiquitin carrier protein E2 C antibody; Ubiquitin carrier protein E2C antibody; Ubiquitin conjugating enzyme E2 C antibody; Ubiquitin conjugating enzyme E2C antibody; Ubiquitin protein ligase C antibody; Ubiquitin-conjugating enzyme E2 C antibody; Ubiquitin-protein ligase C antibody

Species Reactivity

Human

Immunogen

Recombinant Human UBE2C protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG

Clone No.

22E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

UBE2C, also known as UbcH10, is a critical E2 ubiquitin-conjugating enzyme that plays an essential role in cell cycle progression through its involvement in the anaphase-promoting complex/cyclosome (APC/C) pathway. This enzyme facilitates the ubiquitination and subsequent degradation of mitotic cyclins, making it indispensable for proper cell division. Elevated UBE2C expression has been documented across numerous cancer types, positioning it as a significant marker for researchers investigating cell proliferation, tumor biology, and potential therapeutic targets.

This recombinant monoclonal antibody, clone 22E1, offers the reproducibility and consistency that demanding experimental workflows require. Generated using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The mouse IgG2a isotype antibody was raised against recombinant human UBE2C protein and has been affinity-purified to ensure optimal specificity for your target.

Validation studies demonstrate robust performance across multiple applications. Immunohistochemistry testing in paraffin-embedded human colorectal cancer and stomach tissue sections shows clear staining at 1:300 dilution using citrate buffer antigen retrieval, providing researchers studying gastrointestinal malignancies with a reliable detection tool. Flow cytometry analysis in HeLa cells confirms intracellular detection capability, with distinct signal separation from isotype controls when used at 1:100 dilution following formaldehyde fixation and Triton X-100 permeabilization.

Whether you're exploring cell cycle regulation, investigating ubiquitin-proteasome pathway dynamics, or characterizing UBE2C expression patterns in cancer research, this antibody delivers the performance and consistency needed for meaningful experimental outcomes in epigenetics, nuclear signaling, and cell biology applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Gene References into Functions

UBE2C is involved in the anti-proliferative and pro-apoptotic functions of ECRG4 in esophageal squamous cell carcinoma. PMID: 29268240
loss of BRCA1 function results in an increase in UBE2C expression and chemical resistance to doxorubicin in breast cancer cells. PMID: 28881292
FoxM1 promotes glioma progression by enhancing UBE2C transcription PMID: 28767320
SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S ubiquitin-conjugating enzymes to ubiquitylate beta-TrCP1 via K11-linkage for degradation. PMID: 27910872
UBE2C and HOXA1 RNA and protein are differentially expressed in conventional and Spitz nevi and melanoma. PMID: 28657121
High UBE2C expression is associated with esophageal squamous cell carcinoma. PMID: 27588470
Urinary UBE2C cell-free RNA may be a valuable diagnostic marker for bladder cancer. PMID: 27528424
A role was identified for UBE2C as a marker of the androgen signaling pathway in prostate cancer. PMID: 27550197
Data suggest that the activity of AURKA might be regulated by UBE2C through regulating the activity of anaphasepromoting complex. UBE2C may be a new marker in the diagnosis of gastric cancer and may be a potential therapeutic target for the treatment of gastric adenocarcinoma. PMID: 28260026
High UBCH10 expression is associated with bortezomib-resistance in multiple myeloma. PMID: 28000886
the expression of UbcH10 in colorectal cancer samples as compared with healthy tissue sample from the same patient according to age at surgery, was examined. PMID: 26744065
UbcH10 may promote gastric cancer growth. PMID: 27349176
Identification of UBE2C as a target of wild-type and GOF mutant p53 further highlights the contribution of p53 in regulation of spindle assembly checkpoint PMID: 27129209
The anaphase-promoting complex/cyclosome C activity in human cells is tuned by the combinatorial use of three E2 ubiquitin-conjugating enzymes, namely UBE2C, UBE2S, and UBE2D. PMID: 26904940
Study shows an overexpression of UbcH10 mRNA and protein in the vast majority of colorectal cancer (CRC) patients analyzed and indicates that UbcH10 expression regulates CRC growth. PMID: 25917796
the present study showed that miR-196a promoted cell proliferation by targeting UBE2C in breast cancer. Thus, miR-196a may be a potential oncogene in breast cancer and a promising therapeutic target in breast cancer treatment. PMID: 26062455
UBE2C could regulate phospho-ERK1/2 level. PMID: 25832867
Data suggest that ubiquitin-conjugating enzyme UbcH10 may be a promising target for the therapy of breast cancer. PMID: 25739083
Upregulation of miR-17/20a promotes gastric cancer cell growth by targeting UBE2C. PMID: 25760688
UbcH10 overexpression increases carcinogenesis and blocks ALLN susceptibility in colorectal cancer. PMID: 25376843
in addition to binding APC11's RING, UBCH10 is corecruited via interactions with APC2 PMID: 25825779
excess E2F1 due to Rb inactivation recruits the complex of Cdc20 and the anaphase-promoting complex/cyclosome to deregulate the expression of UBCH10 PMID: 25368385
UBE2C may be a marker for diagnosis of nonpalpable breast lesions but not benign or malignant tumors in mammography core biopsies. Suppression of UBE2C may be a potential therapy target in breast cancer. PMID: 24699941
the UBE2C gene is capable of promoting proliferation and invasion of lung cancer cells. Therefore, UBE2C may play a pivotal role in development and invasion of lung cancer. PMID: 24815438
Results suggest that UBE2C acts as an oncogene in prostate cancer progression. PMID: 24512726
Data suggest that ubiquitin conjugating enzyme UBE2C may serve as a potential therapeutic target aimed at inducing radiation and chemo sensitization. PMID: 24072565
Elevated levels of S100A8 and UBE2C were observed. PMID: 24114735
UBE2C positivity is independently associated with shorter cancer-specific survival in bladder cancer patients who underwent radical cystectomy. PMID: 23826418
Data indicate that expression-based risk indices of three genes UBE2C, TPX2, and MELK were more strongly associated with poor 5-year survival in adenocarcinoma patients. PMID: 23357462
High expression of UbcH10 was associated with pancreatic ductal adenocarcinoma. PMID: 23355337
the expression of cyclin D1, MCM7, TRIM29, and UBE2C was found to be significantly associated with progression to muscle-invasive bladder cancer. PMID: 23201130
UbcH10 overexpression has a critical role in lung carcinogenesis, and the evaluation of UbcH10 expression levels may be a new tool for the characterisation of non small cell lung carcinoma. PMID: 23102841
Data sugggest that UbcH10 may play an important role in non-small cell lung cancer (NSCLC) carcinogenesis, and silencing UbcH10 may represent a potential therapeutic strategy for NSCLC. PMID: 22760214
Overexpression of UbcH10 may be a useful indicator of endometrial carcinoma. PMID: 22415060
growth rate of SiHa and HeLa transfected with dominant negative-UBE2C was significantly reduced compared to vector control PMID: 22694363
High UBE2C mRNA expression was found to be of adverse prognostic significance in high-risk breast cancer patients PMID: 22056852
UbcH10 expression was associated with tumor invasion of the portal vein, tumor size, TNM staging, and tumor differentiation in hepatocellular carcinoma. PMID: 21354912
Effect of UBE2C overexpression on the proliferation of 293T cell line PMID: 21651863
Mediator complex subunit (MED)1 phosphorylation leads to UBE2C locus looping, UBE2C gene expression and cell growth in castration-resistant prostate cancer. PMID: 21556051
Spindle assembly checkpoint protein Cdc20 transcriptionally activates expression of ubiquitin carrier protein UbcH10. PMID: 21454660
Elevated expression of UbcH10 is associated with breast cancer. PMID: 19038004
elevated levels of Ubch10 and Cdc20 degrade cyclin B in hpv-16 iinfected cells, required for exit from mitosis, permitting initiation of the next round of DNA synthesis and cell cycle progression. PMID: 20739533
The unstructured N-terminal region of UbcH10 directly binds the 19 S regulatory complex of the 26 S proteasome, and it mediates the initiation of substrate translocation. PMID: 20007692
The overexpression of UbcH10 gene plays a critical role in the carcinogenesis and tumor progression of colorectal cancer. PMID: 19779934
show that UbcH10 overexpression leads to precocious degradation of cyclin B by the anaphase-promoting complex/cyclosome, supernumerary centrioles, lagging chromosomes, and aneuploidy. PMID: 20065091
Data imply that knocking-down UbcH10 protein expression may represent a potential therapeutic option for glioma. PMID: 19657671
UbcH10 is highly expressed in various human primary tumors and UbcH10 has an ability to promote cell growth and malignant transformation. PMID: 12874022
UbcH10 plays an important role in tumor development and that its inhibition in combination with agonists of the TRAIL receptor may provide an enhanced therapeutic index PMID: 15208666
UbcH10 overexpression is involved in thyroid cell proliferation and may represent a marker of thyroid anaplastic carcinomas PMID: 16106252
results showing aberrations in levels of gene expression and locus copy number of ubiquitin-conjugating enzyme E2C gene (UBE2C) suggest that this gene may play an important role in tumor progression leading to advanced colon cancer with liver metastasis PMID: 16772118

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Protein Families

Ubiquitin-conjugating enzyme family

Database Links

HGNC: 15937

OMIM: 605574

KEGG: hsa:11065

STRING: 9606.ENSP00000348838

UniGene: Hs.93002

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Code	CSB-RA025440MA1HU
Size	US$210
	Order now
Image	IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA025440MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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UBE2C Recombinant Monoclonal Antibody

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Target Background