UBE2L3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA212098A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), A549 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes:UBE2L3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time：10s IHC image of CSB-RA212098A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HeLa cell with CSB-RA212098A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells stained with CSB-RA212098A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P68036

Target Names

UBE2L3

Alternative Names

E2 F1 antibody; L UBC antibody; L-UBC antibody; UB2L3_HUMAN antibody; UBCE7 antibody; UbcH7 antibody; UbcM4 antibody; Ube2l3 antibody; Ubiquitin carrier protein L3 antibody; Ubiquitin conjugating enzyme E2 L3 antibody; Ubiquitin protein ligase L3 antibody; Ubiquitin-conjugating enzyme E2 L3 antibody; Ubiquitin-conjugating enzyme E2-F1 antibody; Ubiquitin-protein ligase L3 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human UBE2L3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5H4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

UBE2L3, also known as UbcH7, is a ubiquitin-conjugating enzyme that plays a central role in the ubiquitin-proteasome pathway, facilitating the transfer of ubiquitin to target proteins destined for degradation or functional modification. This E2 enzyme participates in critical cellular processes including cell cycle regulation, DNA repair, and immune signaling, making it a valuable target for researchers investigating protein homeostasis, cancer biology, and inflammatory pathways.

This recombinant monoclonal antibody, clone 5H4, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit host and affinity-chromatography purification ensure high specificity for your target.

Extensive validation demonstrates this antibody's versatility across multiple applications. Western blot analysis confirms reliable detection of UBE2L3 at the expected 18 kDa molecular weight across diverse human cell lines including K562, MCF-7, COLO-205, A549, SH-SY5Y, HepG2, and HeLa cells. Cross-species reactivity extends to mouse and rat brain tissue lysates, supporting translational research using rodent models. Immunohistochemistry validation in paraffin-embedded human lung cancer tissue demonstrates suitability for pathological investigations, while immunofluorescence staining in HeLa cells reveals clear cytoplasmic localization patterns. Flow cytometry analysis in HepG2 cells further expands your experimental options for quantitative single-cell studies.

Whether you are exploring ubiquitin pathway dysregulation in disease contexts, characterizing post-translational modification networks, or investigating epigenetic and nuclear signaling mechanisms, this antibody provides a dependable tool for advancing your research objectives.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.

Gene References into Functions

Crystal structure of HHARI and UbcH7 reveals UbcH7 approximately Ub binding RING1 domain of auto-inhibited HHARI. PMID: 28552575
Data suggest ordered binding of UBCH7-ubiquitin to E6AP Site 1 (for E6AP-Cys820/ubiquitin thioester formation) and E6AP Site 2 (for subsequent chain elongation); proximal indexation accounts for radially symmetric structure of E6AP, requirement for oligomerization in polyubiquitin chain formation, and mechanistic rationale for Cys820-ubiquitin thioester as platform in chain assembly. (E6AP = ubiquitin-protein ligase E6AP) PMID: 28924046
The TCGGC haplotype is associated with an increased risk of Hashimoto Thyroiditis(HT) and UBE2L3 gene is likely to be a susceptibility factor to HT in a Chinese Han population. PMID: 27094594
together with UBE2N and UBE2D2, synergistically contribute to Parkin-mediated mitophagy PMID: 24906799
The MAP1B-LC1 could interact with the ubiquitin-conjugating E2 enzyme UBE2L3 and that the ubiquitination/degradation mechanism triggered by MAP1B-LC1 could be prevented by inhibiting the ubiquitin-proteasome proteolytic pathway. PMID: 25483588
An interaction between LC1 and the ubiquitin-conjugating enzyme UBE2L3. PMID: 24566975
UbcH7 has a role in regulating 53BP1 stability and DSB repair PMID: 25422456
UBE2L3 polymorphism amplifies NF-kappaB activation and promotes plasma cell development, linking linear ubiquitination to multiple autoimmune diseases. PMID: 25640675
Report role of UBE2L3 genetic variants in confering risk of systemic lupus erythematosus in Chinese population. PMID: 24091983
Importance of HLA-C and UBE2L3 in the clearance of HBV infection in addition to HLA-DP and HLA-DQ. PMID: 24162738
The results suggest that variants carried on the SLE-associated UBE2L3 risk haplotype influence autoimmunity by modulating UBCH7 expression. PMID: 22476155
UBE2L3 was found to be a shared susceptibility gene for siffuse cutaneous systemic sclerosis in a Japanese population. PMID: 22294623
The interaction between UBE2L3 genotype and autoantibodies upon serum IFN-alpha suggests a biological role for this locus in patients with systemic lupus erythematosus. PMID: 22045845
Crystal structures of two bacterial E3s, Salmonella SopA and Escherichia coli NleL, both in complex with human E2 UbcH7, are reported. PMID: 22308380
UBCH7 exhibits activity with the RING-in-between-RING (RBR) family of E3s that includes parkin (also known as PARK2) and human homologue of ariadne (HHARI; also known as ARIH1) PMID: 21532592
Genetic variations of UBE2L3 and BCL3 are potential new risk genes for Crohn's disease. PMID: 20601676
UbcH7 charged with ubiquitin has an affinity for E6-associated protein (E6AP) similar to that of uncharged UbcH7. PMID: 20039703
UBE2L3, the c-myc-regulated gene is involved in genotype-C-HBV-related HCC, suggesting that c-myc is related to the hepatocarcinogenic activity of genotype-C HBV. PMID: 16703398
identification of the amino acid residues responsible for the HECT-E2 interaction, and for the dynamical properties of the ubiquitin transfer process, may be of relevant interest for pharmacological and therapeutical purposes PMID: 18805400
These data suggest a role for UbcH7 targets in the completion of cell cycle S phase and entry into G2. PMID: 18946090

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Ubiquitin-conjugating enzyme family

Tissue Specificity

Ubiquitous, with highest expression in testis.

Database Links

HGNC: 12488

OMIM: 603721

KEGG: hsa:7332

STRING: 9606.ENSP00000344259

UniGene: Hs.108104

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Related pathways

Ubiquitin mediated proteolysis

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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