VAMP2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA928839A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), Jurkat whole cell lysate(30µg), U-251MG whole cell lysate(30µg), HEK293 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes:VAMP2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 13 kDa Observed band size: 16 kDa Exposure time：2min IHC image of CSB-RA928839A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA928839A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

P63027

Target Names

VAMP2

Alternative Names

FLJ11460 antibody; RATVAMPB antibody; RATVAMPIR antibody; SYB antibody; SYB2 antibody; Synaptobrevin 2 antibody; Synaptobrevin-2 antibody; VAMP 2 antibody; VAMP-2 antibody; Vamp2 antibody; VAMP2_HUMAN antibody; Vesicle associated membrane protein 2 antibody; Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody; Vesicle-associated membrane protein 2 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human VAMP2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1F5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

VAMP2, also known as synaptobrevin-2, serves as a critical component of the SNARE complex that mediates synaptic vesicle fusion with the presynaptic membrane. This small membrane protein plays an essential role in neurotransmitter release and has broader implications in regulated exocytosis across various cell types, making it a key target for researchers investigating synaptic transmission, vesicle trafficking, and neurological disorders.

This recombinant monoclonal antibody, clone 1F5, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Validation studies demonstrate robust performance across multiple applications and sample types. In western blot analysis, the antibody detects VAMP2 in a diverse panel of human cell lines including A549, SH-SY5Y, Jurkat, U-251MG, and HEK293, as well as mouse and rat brain tissue lysates at dilutions ranging from 1:500 to 1:5000. The observed band at 16 kDa runs slightly higher than the predicted 13 kDa molecular weight, which likely attruibutes to the mobility bias in SDS-PAGE (influence of amino acid composition/conformation) or minor post-translational modifications (such as low-abundance phosphorylation, palmitoylation). This cross-species reactivity with human, mouse, and rat samples provides flexibility for comparative studies and translational research models.

Immunohistochemistry applications have been validated in paraffin-embedded human brain tissue and breast cancer samples using citrate buffer antigen retrieval, demonstrating clear staining at 1:100 dilution. This antibody supports signal transduction research and offers a dependable tool for investigating vesicle-mediated processes in both normal physiology and disease contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.

Gene References into Functions

miR-493-5p overexpression promotes cell apoptosis and inhibits the proliferation and migration of liver cancer cells by negatively regulating the expression of VAMP. PMID: 29328362
VAMP2 is a promising new plasma cell marker PMID: 27247366
VAMP2 is involved in Porphyromonas gingivalis recycling pathway.VAMP2 is localized in early endosomes in gingival epithelial cells. PMID: 26617273
The present study addressed for the first time the unique substrate recognition mechanism of LC/F5 substrate cleavage of VAMP-2 by Botulinum Neurotoxin subtype F5. PMID: 26794648
This study showed that decreased Levels of VAMP2 correlate with Duration of Dementia. PMID: 26639969
VAMP2-NRG1 is a novel oncogenic fusion gene representing a new addition to the list of NRG1 fusion genes, which together may form an important diagnostic and clinical category of lung adenocarcinoma cases PMID: 26134228
A large vesicular pool of VAMP2 maintained by AP180 is crucial to sustain efficient neurotransmission. PMID: 26412491
miR-206 regulates lung surfactant secretion by limiting the availability of VAMP-2 protein. PMID: 25481410
The genetic variations of VAMP2, Synaptotagmin XI might be indication of the relationship between these genes and idiopathic generalized epilepsy PMID: 24164654
BoNT/F5 cleaves substrate synaptobrevin-2 in a different location than the other BoNT/F subtypes, between (54)L and (55)E. PMID: 22172278
unique mechanism of SNARE motif-dependent endocytic sorting and identify the ANTH domain proteins AP180 and CALM as cargo-specific adaptors for synaptobrevin 2 endocytosis PMID: 21808019
VAMP2 mediates the trafficking of alpha5beta1 integrin to the plasma membrane and VAMP2-dependent integrin trafficking is critical in cell adhesion, migration and survival. PMID: 19822142
SNAP-23 and syntaxin-4 are expressed in human eosinophils and are likely candidates for association with VAMP-2 during docking, which is followed by exocytosis PMID: 11842301
VAMP-2 mediates exocytosis of specific and tertiary granules of neutrophils through its interaction with Q-SNARE/R-SNARE complexes located at the neutrophil plasma membrane. PMID: 12517971
VAMP-2 can be one of the v-SNAREs for constitutive exocytosis PMID: 16195891
addition of PIP2 to Stx4/SNAP23 vesicles inhibited the fusion reaction, and its addition to VAMP2 vesicles was stimulatory PMID: 17001002
The vesicular distribution may be attributed in part to the direct interaction between PKD3 and vesicle-associated membrane protein VAMP2, through which PKD3 may regulate VAMP2 vesicle trafficking by facilitating its recruitment to the target membrane. PMID: 17196367
VAMP2 can be phosphorylated by activated PKCzeta in vitro and the presence of ProF increases the PKCzeta-dependent phosphorylation of VAMP2 in vitro PMID: 17313651
Results indicate that the sorting determinants of synaptic vesicle proteins can operate independently of a neuronal context and implicate the association of VAMP2 with synaptophysin I in the specification of the pathway of synaptic vesicle biogenesis. PMID: 17331077
Overexpression of CALM leads to the reduction of cell surface VAMP2, whereas knockdown of CALM by RNA interference results in the accumulation of surface VAMP2. PMID: 18182011
BoNT/B and TeNT possess similar organization but have unique residues to recognize and cleave VAMP-2 PMID: 18511417
analysis of the substrate recognition mechanism of VAMP/synaptobrevin-cleaving clostridial neurotoxins PMID: 18511418
These results do not suggest that a common genetic variant at VAMP2 or VAMP3 contributes to the development of bipolar affective disorder in German patients. PMID: 18628682

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Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell membrane.

Protein Families

Synaptobrevin family

Tissue Specificity

Nervous system and skeletal muscle.

Database Links

HGNC: 12643

OMIM: 185881

KEGG: hsa:6844

STRING: 9606.ENSP00000314214

UniGene: Hs.25348

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Secreted Extracellular Vesicles Synaptic vesicle cycle Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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