VDAC1 Antibody

Code CSB-RA025821A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, LO2 whole cell lysate, Raji whole cell lysate, Rat heart tissue, Mouse brain tissue
    All lanes: VDAC1 antibody at 0.7μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 31 KDa
    Observed band size: 31 KDa

  • IHC image of CSB-RA025821A0HU diluted at 1:73.125 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA025821A0HU diluted at 1:73.125 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Uniprot No. P21796
Target Names VDAC1
Alternative Names N2441 antibody; OMP2 antibody; POR1 antibody; hVDAC1 antibody; MGC111064 antibody; Mitochondrial Porin antibody; Outer mitochondrial membrane protein porin 1 antibody; Plasmalemmal porin antibody; Porin 31HL antibody; Porin 31HM antibody; VDAC antibody; VDAC-1 antibody; Vdac1 antibody; VDAC1_HUMAN antibody; Voltage dependent anion channel 1 antibody; Voltage dependent anion selective channel protein 1 antibody; Voltage-dependent anion-selective channel protein 1 antibody; YNL055C antibody; YNL2441C antibody
Species Reactivity Human, Mouse, Rat
Immunogen A synthesized peptide derived from human VDAC1
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective
Gene References into Functions
  1. VDAC1 allows Ca(2+) access to the MCU, facilitating transport of Ca(2+) to the matrix, and also from the IMS to the cytosol. Intra-mitochondrial Ca(2+) controls energy production and metabolism by modulating critical enzymes in the tricarboxylic acid (TCA) cycle and fatty acid oxidation. PMID: 29594867
  2. associate dysfunction in Fe-S cluster biogenesis with cleavage of VDAC1 PMID: 29596470
  3. HK1 competes with SOD1 G93A mutant from familial amyotrophic lateral sclerosis cases for binding VDAC1.SOD1 G93A mutant from familial amyotrophic lateral sclerosis cases binds VDAC1 with high affinity. PMID: 27721436
  4. Study shows that silencing voltage-dependent anion channel 1 (VDAC1) expression using short interfering RNA-VDAC1 in 9 glioblastoma-related cell lines, including patient-derived cells, led to marked decreases in VDAC1 levels and cell growth. PMID: 28339833
  5. VDAC1 plays an important role in dengue virus infection. PMID: 27779201
  6. VDAC1 is a direct target of miR-320a in non-small cell lung cancer (NSCLC) cells, and miR-320a inhibits VDAC1 expression in NSCLC cells PMID: 27304056
  7. The results of this study shown VDAC1, the Potential Target of miR-320a, is Upregulated in Response to HIV-1 Tat. PMID: 27761954
  8. the present study indicated that VDAC1 may interact with HPV16 E7 to promote the malignant progression of HPV-related cervical cancer PMID: 27419626
  9. Porin expression was lower in patients with heart failure with preserved ejection fraction compared to controls. PMID: 27179829
  10. Studied voltage-dependent anion channel 1 (VDAC1) structure and oligomerization using an Escherichia coli cell-free protein synthesis system and bicelle crystallization. PMID: 28608415
  11. In this study, molecular dynamics simulations and single-channel experiments of VDAC-1 show agreement for the current-voltage relationships of an "open" channel and they also show several subconducting transient states that are more cation selective in the simulations. We observed voltage-dependent asymmetric distortions of the VDAC-1 barrel and the displacement of particular charged amino acids. PMID: 27653481
  12. VDAC1 was accumulated in the desmin highly stained area of muscle fibers of desminopathy patients. PMID: 27941998
  13. work raises the interesting possibility that cholesterol-mediated regulation of VDAC1 may be facilitated through a specific binding site at the functionally important Glu(73) residue. PMID: 28396346
  14. this study describes novel drug candidates with a defined mechanism of action that involves inhibition of VDAC1 oligomerization, apoptosis, and mitochondrial dysfunction. The compounds VBIT-3 and VBIT-4 offer a therapeutic strategy for treating different diseases associated with enhanced apoptosis and point to VDAC1 as a promising target for therapeutic intervention. PMID: 27738100
  15. Results from the simulations show that HK2 binding restricts the movement of the VDAC1 N-terminal helix. As a result, VDAC1 is kept in the open state most of the time and probably allows a constant supply of ATP to HK2 for glycolysis. PMID: 27544294
  16. The findings of this study suggest that inhibition of intracellular Ca(2+/-) overload could protect cells from damage and that VDAC1 plays a considerable role in Cr(VI)-induced liver injury. PMID: 27898307
  17. Our study suggested that miR-7 suppressed the expression of VDAC1 in hepatocellular carcinoma PMID: 26831666
  18. Results shows that beta-barrel of human VDAC1 embedded into a membrane is highly flexible and that Ca2+, a key regulator of metabolism and apoptosis, strongly decreases its plasticity suggesting that physiological VDAC function depends on the molecular plasticity of its channel. PMID: 27021164
  19. High VDAC1 expression is associated with cervical cancer. PMID: 26716410
  20. Studies using B16F10 and A375 cells genetically modified for ATF2 indicated that mitochondrial ATF2 was able to dissociate Bim from the Mcl-1/Bim complex to trigger VDAC1 oligomerization. PMID: 26462148
  21. findings also suggest that VDAC1 may be a novel biomarker for gastric cancer PMID: 26646027
  22. serum starvation induces CREB1 expression, in turn activating miR-320a expression, which then down-regulates VDAC1 expression to facilitate mitophagy PMID: 26472185
  23. Reducing VDAC1 expression induces a non-apoptotic role for pro-apoptotic proteins in glioblastoma multiforme cancer cell differentiation. PMID: 27080741
  24. The works available on VDAC cysteines support the notion that VDAC1, VDAC2, and VDAC3 proteins are paralogs with a similar pore-function and slightly different, but important, ancillary biological functions. (Review) PMID: 26947058
  25. The protective effect of miR-7 is partly exerted through promoting mitochondrial function by targeting VDAC1 expression. PMID: 26801612
  26. Data show that voltage-dependent anion channel 1 (VDAC1) knockout cells are resistant to AMP-activated protein kinase (AMPK) and mechanistic target of rapamycin (mTOR) modulation by itraconazole, indicating VDAC1 is the mediator of the activity. PMID: 26655341
  27. Amyloid beta -mediated toxicity involves mitochondrial and plasma membrane VDAC1, leading to mitochondrial dysfunction and apoptosis induction. PMID: 26542804
  28. PGC-1alpha deficiency exacerbates high glucose-induced apoptosis in human umbilical vein endothelial cells through activation of VADC1. PMID: 26191154
  29. The serum lever of Alzheimer's disease were increase and the expression of VDAC1 strongly correlated with the Mini-Mental State Examination scores of the AD patients. PMID: 25502766
  30. VDAC1 is involved in the process of mitochondria-mediated apoptosis by mediating the release of apoptotic proteins and interacting with anti-apoptotic proteins. (Review) PMID: 25448878
  31. The functional interactions between VDAC and alpha-syn, revealed by the present study, point toward the long sought after physiological and pathophysiological roles for monomeric alpha-syn in PD and in other alpha-synucleinopathies PMID: 26055708
  32. Results show that BNIP3 interacts with the voltage-dependent anion channel (VDAC) to directly induce mitochondrial release and nuclear translocation of EndoG. PMID: 25436615
  33. TP53 regulation of VDAC1 cleavage occurs through mitochondrial Mieap and is dependent on the endolysosomal pH. PMID: 25691661
  34. These data suggest that an interaction between Mcl-1 and VDAC promotes lung cancer cell migration by a mechanism that involves Ca(2+)-dependent reactive oxygen species production. PMID: 25341036
  35. data indicate that the BH4 domain of Bcl-XL, but not that of Bcl-2, selectively targets VDAC1 and inhibits apoptosis by decreasing VDAC1-mediated Ca(2+) uptake into the mitochondria PMID: 25681439
  36. Voltage-dependent structural changes of hVDAC1 PMID: 24728177
  37. VDAC1 was expressed and reconstituted into two-dimensional lipid crystalline bilayers with characteristics identical to wild type samples. PMID: 25545271
  38. Results indicate that mitochondrial function associated with VDAC1 is decreased in sporadic and experimental Parkinson's disease, and this decrease is associated with alpha-synuclein accumulation and aggregation PMID: 24825319
  39. Data indicate that voltage-dependent anion channel 1 (VDAC1) is involved in plasminogen kringle 5 (K5)-induced activation of the mitochondrial apoptosis pathway. PMID: 25296756
  40. Data indicate that curcumin interacts with residues in the alpha helical N-terminus of voltage dependent anion channel VDAC-1 and in the channel wall. PMID: 25459681
  41. Ca(2+)-mediated regulation of VDAC1 expression levels is associated with cell death induction. PMID: 24704533
  42. Label-free quantitative comparison of DN urinary exosomes vs control group and SRM further validation, resulted in the discovery of a panel of three proteins (AMBP, MLL3 and VDAC1) which changes in DN. PMID: 24211404
  43. the C-terminus end of VDAC faces the mitochondrial inter-membrane space. PMID: 24324700
  44. This review examines the significance of this new form of VDAC1 for anticancer therapy.[review] PMID: 24272356
  45. Nucleotide interactions of the human voltage-dependent anion channel. PMID: 24668813
  46. Increase in mRNA levels of the voltage-dependent anion channel 1 gene is associated with Alzheimer's disease. PMID: 24063855
  47. Abnormal interaction of VDAC1 with amyloid beta and phosphorylated tau causes mitochondrial dysfunction in Alzheimer's disease. PMID: 22926141
  48. VDAC binds tissue-type plasminogen activator (t-PA) on human neuroblastoma SK-N-SH cells PMID: 23161549
  49. VDAC 1, 2, and 3 recruit Parkin to defective mitochondria to promote mitochondrial autophagy. PMID: 23060438
  50. The N-terminal helix of VDAC1 controls entry into elliptic beta-barrel states which underlie VDAC closure. PMID: 22841291
  51. Interfering with Bcl-xL binding to the mitochondria by VDAC1-based peptides may serve to induce apoptosis in cancer cells. PMID: 22589539
  52. analysis of lipid dynamics and protein-lipid interactions in 2D crystals formed with the beta-barrel integral membrane protein VDAC1 PMID: 22435461
  53. A single cysteine-residue-bearing VDAC1 demonstrates that the N-terminal region lies inside the channel pore. PMID: 22397371
  54. there are two possible mechanisms triggered by MAP4: stabilization of MT networks; DYNLT1 modulation, which is connected with VDAC1, and inhibition of hypoxia-induced mitochondrial permeabilization PMID: 22164227
  55. PBR can inhibit endothelial activation through inhibition of mitochondrial ROS and/or VDAC-1 expression in endothelial cells PMID: 22616995
  56. specific lipid composition of the outer mitochondrial membrane might be of crucial relevance and, thus, a potential cue for regulating the oligomeric state of VDAC PMID: 22325275
  57. VDAC1 and CNTNAP1 associate with gamma-secretase in detergent-resistant membranes and affect amyloid precursor protein processing. PMID: 22315232
  58. hypoxia induces the appearance of a C-terminal truncated form of VDAC1 PMID: 22389449
  59. VDAC1 silencing potentiates apoptotic challenges. PMID: 21720385
  60. VDAC is the ATP conduit in the IP receptor-mediated signaling pathway in human erythrocytes. PMID: 22159995
  61. Dissection of VDAC1 dimerization/oligomerization as presented here provides structural insight into the oligomeric status of cellular VDAC1 under physiological and apoptotic conditions. PMID: 22117062
  62. Considering the ubiquitous expression modus of APP, beta- and gamma-secretases and type-1 VDAC/eukaryotic porin a basic model of apoptosis might be given. PMID: 21442216
  63. A total of 1.77 mus molecular dynamics simulations of human VDAC isoform 1, was performed. PMID: 21281574
  64. Canonical Monte Carlo/Brownian dynamics (GCMC/BD) simulations to explore the ion transport properties of human VDAC isoform 1 (hVDAC1; PDB:2K4T) embedded in an implicit membrane. PMID: 21281575
  65. Mitochondria are aggregated by p62, following its recruitment by Parkin in a VDAC1-independent manner. PMID: 20890124
  66. Results imply that micro- to millisecond dynamics in the N-terminal part of the barrel are essential for VDAC interaction and gating. PMID: 21148773
  67. The results of this study demonstrate, for the first time, the anticancer therapeutic potential of VDAC1 downregulation by means of shRNA. PMID: 20404552
  68. Data suggest that VDAC1 oligomerization is involved in mitochondrion-mediated apoptosis and may represent a general mechanism common to numerous apoptogens acting via different initiating cascades. PMID: 20937774
  69. VDAC1 expression was not altered cervical neoplasms. PMID: 20846459
  70. describe the crystallization of voltage-dependent anion channel-1 produced by a bacterial cell-free expression system PMID: 20435015
  71. The substitution of the VDAC3 N-terminus with the VDAC1 N-terminus caused the chimaera to become more active than VDAC1. PMID: 20434446
  72. H. pylori VacA p58 (vacuolating cytotoxin A p58 domain) induced apoptosis in gastric epithelial carcinoma cells and was associated with the up-regulation of Bax/VDAC1 (voltage-dependent anion channel 1) and the downregulation of Bcl-2 PMID: 20132430
  73. these data provide a mechanism to explain how Nek1 regulates cell death by affecting the opening and closing of VDAC1. PMID: 20230784
  74. Data suggest that the two cysteine residues are not required for apoptosis or VDAC1 oligomerization. PMID: 20192921
  75. FimA strengthens the VDAC1-hexokinase interaction and prevents dissociation of hexokinase from VDAC1 triggered by apoptotic stimuli. PMID: 20347420
  76. The dynamics of the molecular interactions between the C-terminal region of PB1-F2 protein and VDAC1 and ANT3 were expounded by employing an in silico approach. PMID: 19669810
  77. The length of the tag at the C-terminus is critical for the assembly of VDAC1, as well as the amino acid residues at positions 130, 222, 225 and 251 of the protein. PMID: 20117113
  78. Data identified VDAC1 (voltage-dependent anion channel 1) as a target for Parkin-mediated Lys 27 poly-ubiquitylation and mitophagy. PMID: 20098416
  79. Our data thus support a model of the PTP complex involving VDAC1 at the MOM, and indicate that VDAC1-dependent MPT is an upstream mechanism playing a causal role in oxidative stress-induced apoptosis. PMID: 19668262
  80. interacts with the dynein light chain Tctex1 and the heat-shock protein PBP74; results of this study represent additional evidence of membrane-associated trafficking of the voltage-dependent anion-selective channel 1 PMID: 12009301
  81. Activation of mitochondrial voltage-dependent anion channel by apro-apoptotic protein Bim PMID: 12118373
  82. Presence of a plasma membrane VDAC was demonstrated. Colocalisation of VDAC with caveolin-1. PMID: 12698369
  83. is a receptor for plasminogen kringle 5 on endothelial cells PMID: 12736244
  84. Identification of the protein-protein contact site and interaction mode of VDAC1 with Bcl-2 family proteins. PMID: 12767928
  85. a major function of VDAC1 in the plasma membrane is that of a NADH(-ferricyanide) reductase that may be involved in the maintenance of cellular redox homeostasis PMID: 14573604
  86. VDAC homodimerization could potentially determine its gating capacity to cyto c, and Bcl-2 blockage of VDAC homodimerization represents a novel mechanism for its inhibition of apoptosis. PMID: 14647451
  87. a model on how electron transport may occur in VDAC1. PMID: 15630200
  88. evidences of VDAC-COX interaction in vitro proved to be functional as evidenced by the effect of the human and yeast isoforms of VDAC on the oxidation of cytochrome c by the pure holoenzyme and by the effect of the COX epitope on VDAC permeability PMID: 16185087
  89. VDAC1 regulates apoptosis independent of the apoptosis-inducing pathway. PMID: 16585511
  90. Bcl-xL can bind to one or two VDAC1 molecules forming heterodimers and heterotrimers. PMID: 17209561
  91. gelsolin G5 domain inhibits HIV-Vpr-induced T-cell apoptosis by blocking the interaction between Vpr and VDAC PMID: 17254575
  92. hVDAC1 formed different structures in detergent micelles and phospholipid bilayers. PMID: 17336328
  93. Our analysis strongly suggests a 16-stranded, antiparallel beta-barrel with one large and seven short loops and turns. Initial crystallization trials of the protein yielded crystals diffracting to 8 Angstrom resolution. PMID: 17828567
  94. phosphorylated StAR interacts with voltage-dependent anion channel 1 (VDAC1) on the OMM, which then facilitates processing of the 37-kDa phospho-StAR to the 32-kDa intermediate. PMID: 18250166
  95. interference with the binding of Hexokinase-I to mitochondria by VDAC1-derived peptides may offer a novel strategy by which to potentiate the efficacy of conventional chemotherapeutic agents PMID: 18308720
  96. VDAC1 may act as a facultative regulator/effector of MMP, depending on the initial cytotoxic event. PMID: 18362892
  97. two serine residues of VDAC1, Ser-12 and Ser-103, can modulate VDAC1 protein level and thus the sensitivity to apoptosis stimuli PMID: 18381814
  98. Human voltage-dependent anion selective channel 1 is a target antigen for anti-glomerular endothelial cell antibody in mixed connective tissue disease. PMID: 18568384
  99. High-resolution structure analysis of human VDAC1 crystal lattice reveals that it belongs to trigonal space group P321, with unit-cell parameters a = 78.9, c = 165.7 A and one monomer in the asymmetric unit. PMID: 18607100
  100. Approaching the structure of human VDAC1, a key molecule in mitochondrial cross-talk. PMID: 18690523

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Subcellular Location Mitochondrion outer membrane, Multi-pass membrane protein, Cell membrane, Multi-pass membrane protein, Membrane raft, Multi-pass membrane protein
Protein Families Eukaryotic mitochondrial porin family
Tissue Specificity Heart, liver and skeletal muscle.
Database Links

HGNC: 12669

OMIM: 604492

KEGG: hsa:7416

STRING: 9606.ENSP00000265333

UniGene: Hs.519320

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