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The preparation of the VDAC1 recombinant monoclonal antibody involves the utilization of DNA recombinant technology and in vitro genetic manipulation. The process initiates with the immunization of animals using a synthesized peptide derived from human VDAC1, followed by the isolation and selection of positive B cells. Single clone identification and screening of these positive B cells are performed. The light and heavy chains of the VDAC1 antibody are then amplified using PCR and inserted into a plasmid vector. This recombinant vector is subsequently transfected into a host cell line for antibody expression. The VDAC1 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It exhibits strong reactivity with VDAC1 protein from human, mouse, and rat samples and is particularly suitable for ELISA, WB, and IHC applications.
The VDAC1 protein is a mitochondrial outer membrane protein that functions as a channel for the transport of small metabolites, ions, and signaling molecules between the cytosol and the mitochondrial intermembrane space. It is involved in the regulation of cellular metabolism, apoptosis, and autophagy. VDAC1 is essential for energy production in cells, as it facilitates the transport of ATP, ADP, and other nucleotides across the mitochondrial membrane. It also plays a role in the regulation of mitochondrial permeability, which is important for the release of cytochrome c and other pro-apoptotic factors from the mitochondria during apoptosis. Additionally, VDAC1 has been implicated in the regulation of mitochondrial dynamics, mitochondrial biogenesis, and the response to oxidative stress.
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