VSIG4 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA896869MA2HU
Size	US$210
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Image	IHC image of CSB-RA896869MA2HU diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA896869MA2HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
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Target Background

Product Details

Uniprot No.

Q9Y279

Target Names

VSIG4

Alternative Names

Complement receptor of the immunoglobulin superfamily antibody; CRIg antibody; Ig superfamily protein antibody; Protein Z39Ig antibody; UNQ317/PRO362 antibody; V set and immunoglobulin domain containing 4 antibody; V-set and immunoglobulin domain-containing protein 4 antibody; VSIG4 antibody; VSIG4_HUMAN antibody; Z39IG antibody

Species Reactivity

Human

Immunogen

Recombinant Human VSIG4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

16G9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

VSIG4, also known as complement receptor of the immunoglobulin superfamily (CRIg), serves as a critical regulator of innate immunity expressed predominantly on tissue-resident macrophages. This immunoglobulin superfamily member functions as a pattern recognition receptor that directly binds complement component C3b and its cleavage fragments, enabling macrophages to efficiently clear complement-opsonized pathogens and apoptotic cells. Beyond its role in pathogen clearance, VSIG4 has emerged as an important negative regulator of T cell activation, positioning it at the intersection of innate and adaptive immunity with significant implications for autoimmune disease, transplant tolerance, and tumor immunology research.

This recombinant monoclonal antibody, clone 16G9, offers the reproducibility and consistency that demanding immunological studies require. Generated against recombinant human VSIG4 protein and produced using recombinant technology with a human IgG1 framework, this antibody delivers sequence-defined specificity that eliminates the lot-to-lot variability inherent in traditional hybridoma-derived reagents. Affinity chromatography purification ensures high purity suitable for sensitive detection applications.

Validation studies demonstrate reliable performance across multiple experimental platforms. Immunohistochemistry on paraffin-embedded human placenta tissue, where tissue-resident macrophages abundantly express VSIG4, shows specific staining at dilutions of 1:50 to 1:200 using citrate buffer antigen retrieval. Immunofluorescence analysis in A549 cells confirms utility for cellular localization studies, revealing clear signal with DAPI counterstaining at 1:30 dilution.

For researchers investigating macrophage biology, complement-mediated immunity, or the immunomodulatory functions of VSIG4 in disease contexts, this antibody provides a dependable tool for characterizing expression patterns in human tissues and cell models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.

Gene References into Functions

Soluble VSIG4 levels are associated with the progression and recurrence of ovarian cancer, indicating that soluble VSIG4 may be used as a potential biomarker for predicting tumor prognosis. PMID: 28498255
VSIG4 signaling provides an anti-immune evasion mechanism that prevents the outgrowth of intracellular bacteria in macrophages PMID: 27440002
The VSIG4 upregulation by LMP1 was regulated at the transcriptional level via the NF-kB signaling axis. PMID: 28859984
VSIG4 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
we concluded that let-7g-5p inhibits epithelial-mesenchymal transition (EMT) consistent with reduction of glioma stem cell (GSC) phenotypes by targeting VSIG4 in glioblastoma. PMID: 27634309
Data indicate that rotein kinase calpha (PKCalpha) plays a role in downregulating complement receptor Ig (CRIg coded by V-set and Ig domain-containing protein 4 VSIG4) expression. PMID: 25687755
complement receptor of the immunoglobulin superfamily-L-factor H protects glomerular mesangial cells from complement-mediated injury and proliferative lesions PMID: 25114177
we identified VSIG4 as a potential diagnostic marker of severe preeclampsia. The determination of this gene may improve the prognostic assessment of severe preeclampsia. PMID: 24349325
Data indicate that massive V-set and Ig domain-containing 4 VSIG4(+) cell infiltration throughout the non-small-cell lung cancer samples. PMID: 24862966
we showed that a complement receptor of the Ig superfamily (CRIg, also known as Z39Ig), a receptor for complement fragments (C3b and iC3b), was expressed on a subset of intestinal macrophages in murine and human large intestine PMID: 21768202
These results suggest that T cells can opposite T cell hyporesponsiveness through dampening Z39Ig inhibitory signals from macrophages and thus maintain their anti-viral function in chronic hepatitis B. PMID: 20399148
hVSIG4 recombinant adenovirus-transfected DCs suppress T cell proliferation, cytokine production and activation marker expression with PMID: 19914289
Results report the identification and characterization of a Complement Receptor of the Immunoglobulin superfamily, CRIg, that binds complement fragments C3b and iC3b. PMID: 16530040
These data indicate that the macrophage Z39Ig is involved in the pathogenesis of inflammatory diseases through chemokine induction, which will promote the migration of inflammatory cells into the lesion area, and MMP-9 induction. PMID: 16882875
The specific expression of VSIG4 on resting macrophages suggests that VSIG4 may be important for the maintenance of T cell unresponsiveness in healthy tissues. PMID: 17016562
CRIg is not only a phagocytic receptor, but also a potent inhibitor of the alternative pathway convertases PMID: 17051150

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages.

Database Links

HGNC: 17032

OMIM: 300353

KEGG: hsa:11326

STRING: 9606.ENSP00000363869

UniGene: Hs.8904

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Related pathways

Complement and coagulation cascades

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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