Product Details

Uniprot No.

Target Names

Alternative Names

5''-deoxyribose-5-phosphate lyase Ku70 antibody; 5''-dRP lyase Ku70 antibody; 70 kDa subunit of Ku antigen antibody; ATP dependent DNA helicase 2 subunit 1 antibody; ATP dependent DNA helicase II 70 kDa subunit antibody; ATP-dependent DNA helicase 2 subunit 1 antibody; ATP-dependent DNA helicase II 70 kDa subunit antibody; CTC box binding factor 75 kDa subunit antibody; CTC box-binding factor 75 kDa subunit antibody; CTC75 antibody; CTCBF antibody; DNA repair protein XRCC6 antibody; G22P1 antibody; Ku 70 antibody; Ku autoantigen p70 subunit antibody; Ku autoantigen, 70kDa antibody; Ku p70 antibody; Ku70 antibody; Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody; Kup70 antibody; Lupus Ku autoantigen protein p70 antibody; ML8 antibody; Thyroid autoantigen 70kD (Ku antigen) antibody; Thyroid autoantigen antibody; Thyroid lupus autoantigen antibody; Thyroid lupus autoantigen p70 antibody; Thyroid-lupus autoantigen antibody; TLAA antibody; X ray repair complementing defective repair in Chinese hamster cells 6 antibody; X-ray repair complementing defective repair in Chinese hamster cells 6 antibody; X-ray repair cross-complementing protein 6 antibody; XRCC 6 antibody; Xrcc6 antibody; XRCC6_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human XRCC6

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Gene References into Functions

KU70 defect influenced the expression of P53, BCL2 and BAX, remaining Jurkat cells in a pro-apoptosis status and KU70 silencing prolonged the survival time and impaired the tumorigenesis ability in Jurkat-xenografted mice. PMID: 30273928
Small cell lung cancer (SCLC) subtype had amplified risk with XRCC7 6721G>T. Gene-environment interaction analysis revealed that XRCC6 61C>G showed a strong protective effect towards lung cancer. Survival analysis revealed poor prognosis in case of XRCC6 61C>G SCLC subtype. XRCC7 6721G>T subjects with SCLC subtype showed an increased susceptibility while poor prognosis in case of XRCC6 61C>G. PMID: 29397516
verexpression of USP50 has no effect on Ku70 mRNA levels, while it reduces Ku70 protein levels by promoting Ku70 degradation, suggesting that USP50 may indirectly regulate Ku70 protein stability. PMID: 29101126
Observations suggest that IER5 is a novel regulator of the non-homologous end-joining pathway pathway for DNA double-strand breaks repair, possibly through its interaction with PARP1 and Ku70. PMID: 29104487
The DNA binding domain of Ku70 was essential for formation of the Ku70-STING complex. Knocking down STING in primary human macrophages inhibited their ability to produce IFN-lambda1 in response to transfection with DNA or infection with the DNA virus HSV-2 (herpes simplex virus-2). Together, these data suggest that STING mediates the Ku70-mediated IFN-lambda1 innate immune response to exogenous DNA or DNA virus infection. PMID: 28720717
analysis of a novel cellular stress response mechanism in cancer cells and a key role of LSD1/SIRT1/KU70 dynamic interaction in regulating DNA repair and mutation acquisition PMID: 27384990
Single nucleotide polymorphisms in the Ku70 gene XRCC6 were independently associated with Elevated Creatine Kinase Levels in Unhealthy Male Nonagenarians. PMID: 26913518
Although PAXX-deficient cells lack c-NHEJ phenotypes, PAXX forms a stable ternary complex with Ku bound to DNA. Thus, PAXX plays an accessory role during c-NHEJ that is largely overlapped by XLF's function. PMID: 27705800
HTLV-1 infection enhanced the association between Ku70 and stimulator of IFN genes, suggesting that stimulator of IFN genes was involved in Ku70-mediated host defenses against HTLV-1 infection; findings suggest a new sensor that detects HTLV-1 reverse transcription intermediate and controls HTLV-1 replication PMID: 28821586
Nuclear PTEN interferes with binding of Ku70 at double-strand breaks through post-translational poly(ADP-ribosyl)ation. PMID: 27741411
Results show that DDB2 is critical for chromatin association of XRCC5/6 in the absence of DNA damage and provide evidence that XRCC5/6 are functional partners of DDB2 in its transcriptional stimulatory activity. PMID: 28035050
High KU70 expression is associated with drug resistance in glioblastoma. PMID: 27593939
These results suggest that Ku-mediated repression of p53 mRNA translation constitutes a novel mechanism linking DNA repair and mRNA translation. PMID: 26964895
VCP removes sterically trapped Ku70/80 rings from DNA in double-strand break repair. PMID: 27716483
found that the rs228593, rs2267437 and rs1805388 functional polymorphisms probably alter the level of expression of the ATM, XRCC6 and LIG4 genes, respectively, being important in the maintenance of genomic instability in MDS PMID: 27497341
Data show that the increased acetylation of Ku autoantigen 70kDa (Ku70) in sirtuin 6 protein (SIRT6)-depleted cells disrupt its interaction with Bax apoptosis regulator protein (Bax), which finally resulted in Bax mitochondrial translocalization. PMID: 28238784
Ku70 (XRCC6) was found to bind directly within the CR4 of E1A from human adenovirus type 5. PMID: 27769014
periodic phosphorylation of Ku70 by cyclin-cyclin dependent kinases prevents the interaction of Ku with replication origin after initiation events in S-phase. PMID: 27402161
The results of this preclinical study imply that Ku70 might be a primary resistance factor of gemcitabine, and Ku70 silence could significantly chemo-sensitize gemcitabine in pancreatic cancer cells. PMID: 28153717
results reveal that Src plays a protective role against hyperactive apoptotic cell death by reducing apoptotic susceptibility through phosphorylation of Ku70 at Tyr-530. PMID: 27998981
High Expression of XRCC6 Promotes Human Osteosarcoma Cell Proliferation through the beta-Catenin/Wnt Signaling Pathway. PMID: 27455247
Data show that X-ray repair cross-complementing protein 6 ( Ku70) is found to interact with closed circular DNA. PMID: 27825805
The rs2267437 in XRCC6 was associated with increased initial DSB damage. PMID: 26974709
No significant difference in genotype distribution was found between the colorectal cancer patients and controls, or any significant association with cancer-specific or disease-free survival in patients. PMID: 27328741
Ku70 expression may play an important role in hepatocellular carcinoma development PMID: 26797321
Results show that under hypoxia, Ku70 and DNA-PKcs interact with nuclear RON which activates non-homologous end joining DNA repair conferring chemoresistance. PMID: 26772202
We believe that for the first time, genetic variants at XRCC6 and MVP genes are associated with risk of more aggressive disease, and would be taken into account when assessing the malignancy of prostate cancer PMID: 26754263
XRCC6 may play an important role in the carcinogenesis of NPC and could serve as a chemotherapeutic target for personalized medicine and therapy. PMID: 26149939
these data unveil an involvement of phospho-Ku70 in fast but inaccurate DNA repair; a new paradigm linked to both the deregulation of canonical nonhomologous end-joining and the resistance of malignant cells PMID: 26337656
Ku is intact and retains DNA binding activity in early apoptotic cells. PMID: 26976509
Data suggest that heat shock factor 1 (HSF1) interacts with both Ku autoantigens Ku70 and Ku86 to induce defective non-homologous end joining (NHEJ) repair activity and genomic instability. PMID: 26359349
Our results demonstrate that the phosphorylation-mediated dissociation of Ku70/80 from DSBs frees DNA ends, allowing the initiation of HR in S phase and providing a mechanism of DSB repair pathway choice in mammalian cells. PMID: 26712563
retinoblastoma tumor suppressor protein variants disabled for the interaction with XRCC5 and XRCC6, including a cancer-associated variant, are unable to support canonical non-homologous end-joining despite being able to confer cell-cycle control PMID: 25818292
the results of the present study suggested that Ku70 acetylation mediated TSA-induced apoptosis in CRC cells. In addition, Ku70 was found to be indispensable in TSA-induced apoptosis due to its role in protecting Bax from proteosomal degradation. PMID: 25695595
A direct interaction between Ku70/86 and BRG1 brings together SWI/SNF remodeling capabilities and TOP2beta activity to enhance the transcriptional response to hormone stimulation. PMID: 26055322
Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination. PMID: 24769731
loss of expression correlates with decreased survival in gall bladder malignancies patients PMID: 25046228
SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax. PMID: 25299772
Polymorphism in XRCC6 gene is associated with Systemic Lupus Erythematosus. PMID: 25756210
Both the CG carriers/G allele carriers of rs2267437 (XRCC6) and the haplotype AT/CC established by the SNPs of XRCC5 are associated with ESCC (Esophageal Squamous Cell Carcinoma) susceptibility. PMID: 25702660
EEF1A1, SSRP1, and XRCC6 are novel interacting partners of the mineralocorticoid receptor PMID: 25000480
single nucleotide polymorphisms in promoter region might play different roles in various cancers [review] PMID: 25569644
RECQL4 stimulates higher order DNA binding of Ku70/Ku80 to a blunt end DNA substrate. Taken together, these results implicate that RECQL4 participates in the NHEJ pathway of DSB repair via a functional interaction with the Ku70/Ku80 complex. PMID: 24942867
the VNTR polymorphism at the promoter region of XRCC5, but not XRCC6, may have a role in breast cancer risk or age at diagnosis of breast cancer PMID: 24615008
SET/TAF-Ibeta interacts with Ku70/80 in the nucleus and inhibits Ku70 acetylation. PMID: 24305947
A single nucleotide polymorphism that tags both the XRCC6 and SREBF2 genes strongly modifies the association between bladder cancer risk and smoking PMID: 24382701
Enhanced DNA-PKcs and Ku 70/80 expression may be closely associated with gastric carcinoma. PMID: 24187467
This meta-analysis suggests that the XRCC6 rs2267437 polymorphism may affect breast cancer susceptibility and increase the risk of cancer in Asian populations and in the general population. PMID: 23745766
findings suggest that the XRCC6 genotype could serve as a predictor of childhood leukemia risk and XRCC6 could serve as a target for personalized medicine and therapy PMID: 24324074
KU70/KU80 may play a role in DNA DSBs repair in HR-deficient tumours. Further study of other NHEJ markers in sporadic BC is warranted. PMID: 23624778

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Subcellular Location

Nucleus. Chromosome.

Protein Families

Ku70 family

Database Links

HGNC: 4055

OMIM: 152690

KEGG: hsa:2547

STRING: 9606.ENSP00000352257

UniGene: Hs.292493

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Code	CSB-RA076471A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: JURKAT whole cell lysate(30µg), HEK293 whole cell lysate(30µg), A431 whole cell lysate(30µg), Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A549 whole cell lysate(30µg) All lanes: Ku70 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time：2min IHC image of CSB-RA076471A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing HepG2 cells stained with CSB-RA076471A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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XRCC6 Recombinant Monoclonal Antibody

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