XRCC6 Antibody

Datasheet
Code CSB-PA01617A0Rb
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate
    All lanes: XRCC6 antibody at 4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 70, 66 kDa
    Observed band size: 70 kDa

  • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA01617A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA01617A0Rb at dilution of 1:100

  • Immunofluorescent analysis of PC-3 cells using CSB-PA01617A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Product Details

Full Product Name Rabbit anti-Human XRCC6 Polyclonal antibody
Uniprot No. P12956
Target Names XRCC6
Alternative Names X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5\\\'-deoxyribose-5-phosphate lyase Ku70) (5\\\'-dRP lyase Ku70) (70 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CTCBF) (DNA repair protein XRCC6) (Lupus Ku autoantigen protein p70) (Ku70) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 6), XRCC6, G22P1
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human X-ray repair cross-complementing protein 6 ptotein (2-609AA)
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection. Garcia-Moreno M, et al,Molecular Cell,2019

Applications: WB
Review: Western blotting analysis with specific antibodies of the eluates of a representative RIC experiment in SINV-infected HEK293 cells.
PMID: 30799147

Target Data

Function Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Subcellular Location Nucleus, Chromosome
Protein Families Ku70 family
Database Links

HGNC: 4055

OMIM: 152690

KEGG: hsa:2547

STRING: 9606.ENSP00000352257

UniGene: Hs.292493

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