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YWHAE Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA115871A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), A431 whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Jurkat whole cell lysate(30µg), U-937 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Mouse brain tissue lysate(30µg)
    All lanes: 14-3-3 epsilon antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 29 kDa
    Observed band size: 29 kDa
    Exposure time：1min
  • IHC image of CSB-RA115871A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Overlay Peak curve showing Hela cells stained with CSB-RA115871A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P62258
Target Names
YWHAE
Alternative Names
14-3-3 protein epsilon (14-3-3E), YWHAE
Species Reactivity
Human, Mouse
Immunogen
A synthesized peptide from human YWHAE protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
8D9
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

YWHAE, also known as 14-3-3 protein epsilon, belongs to the highly conserved 14-3-3 protein family that plays essential roles in signal transduction, cell cycle regulation, and apoptotic pathways. Through phosphoserine/phosphothreonine-binding interactions, YWHAE modulates the activity of numerous signaling proteins, making it a significant target in cancer biology, neuroscience, and cell signaling research.

This recombinant monoclonal antibody, generated from clone 8D9, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host, researchers benefit from lot-to-lot uniformity that eliminates the variability often encountered with traditional hybridoma-derived antibodies. This reliability is particularly valuable for longitudinal studies or when standardizing protocols across research groups.

Validation data demonstrates robust performance across multiple applications. In western blot analysis, the antibody detects a clean 29 kDa band matching the predicted molecular weight of YWHAE, confirmed across an extensive panel of human cell lines including HeLa, MCF-7, A431, HEK293T, Jurkat, and U-937. Cross-species reactivity with mouse samples has been validated in both NIH/3T3 cells and mouse brain tissue lysate, supporting translational research using murine models. Immunohistochemistry staining in paraffin-embedded human glioma tissue reveals clear target localization, while flow cytometry analysis in HeLa cells shows distinct positive signal separation from isotype controls.

The antibody's versatility across western blot, immunohistochemistry, and flow cytometry applications provides flexibility for researchers investigating 14-3-3 protein function in oncology, neurodegeneration, or fundamental cell biology studies where consistent, reliable detection of YWHAE is essential.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.
Gene References into Functions
  1. results support YWHAE being a risk gene for Major Depressive Disorder in the Han Chinese population. PMID: 28414084
  2. YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells. PMID: 27863420
  3. we report recurrent BCOR exon 16 internal tandem duplications and YWHAE-NUTM2B fusions in half of infantile soft tissue undifferentiated round cell sarcoma and most primitive myxoid mesenchymal tumor of infancy cases, but not in other pediatric sarcomas. PMID: 26945340
  4. For metastatic YWHAE-rearranged HG-ESS, prolonged disease control following diagnosis was seen, with notable responses to anthracycline-based therapy. This emphasizes the need for appropriate molecular testing of uterine mesenchymal malignancies. PMID: 28390819
  5. phosphorus NMR and time-resolved tryptophan fluorescence measurements suggest that 14-3-3zeta interacts with the kinase domain of ASK1 in close proximity to its active site, thus indicating this interaction might block its accessibility and/or affect its conformation. PMID: 27514745
  6. The 14-3-3 family is dysregulated in schizophrenia, perhaps owing to specific regulatory mechanisms; the expression of the 14-3-3 epsilon, theta and zeta isoforms could be useful indicators of disease severity. PMID: 27030512
  7. Studies show that patients with clear cell sarcoma of the kidney (CCSK) and the fusion YWHAE-NUTM2B/E were relatively young, had low tumor volumes, and did not present with stage I disease which fail to identify an explicit clinical phenotype. PMID: 26542179
  8. Data show that the NS3 protein of dengue virus bound to 14-3-3 epsilon protein (14-3-3varepsilon) and prevented translocation of retinoic acid-inducible gene-I protein (RIG-I) to the adaptor MAVS protein and thereby blocked antiviral signaling. PMID: 26998762
  9. Report mutually exclusive BCOR internal tandem duplications and YWHAE-NUTM2 fusions in clear cell sarcoma of kidney. PMID: 27000436
  10. CEP131 is the key regulatory target of MK2 and 14-3-3 in centriolar satellite remodeling. PMID: 26616734
  11. 14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
  12. DAPK2-induced apoptosis is negatively regulated by Akt and 14-3-3 proteins. PMID: 26047703
  13. These data demonstrate a novel role for HDAC6 in controlling 14-3-3zeta binding activity. PMID: 25770209
  14. YWHAE-FAM22 rearrangement is associated with endometrial stromal sarcomas. PMID: 25244606
  15. Results suggest a specific role for YWHAE single nucleotide polymorphisms in the pathophysiology of schizophrenia. PMID: 25105667
  16. Cleft lip/palate has been found associated with 17p13.3 duplication involving a single candidate gene (YWHAE) in a father and his son. PMID: 23901910
  17. Data show that two isoforms of the 14-3-3 family, 14-3-3epsilon and 14-3-3 gamma, can stabilize Cdt2 independent of each other when overexpressed. PMID: 25154416
  18. These results suggest a possible role for the YWHAE genotype in the early development of the OFC sulcogyral pattern in patients with schizophrenia. PMID: 24561237
  19. data indicate that 14-3-3 is required for Bim expression and that it has an anti-cancer effect under pERK1/2 inhibition in 143B cells PMID: 24536031
  20. for endometrial sarcoma cases with high-grade morphology we recommend to test for YWHAE rearrangements by FISH break-apart PMID: 23599159
  21. YWHAE polymorphisms may be a genetic risk factor for HIV-associated neurocognitive disorder (HAND). PMID: 23982958
  22. Data indicate that 14-3-3 zeta, gamma, epsilon, and tau isoforms but not the sigma protein hydrolyze ATP. PMID: 24269678
  23. Carriers of the YWHAE*C allele of rs3752826 polymorphism have increased risk of suicidal behavior. PMID: 24450199
  24. findings suggest that FAK expression is correlated with and upregulated by 14-3-3epsilon via activation of NFkappaB PMID: 22934705
  25. interaction between NAV2 and 14-3-3epsilon PMID: 24161943
  26. 14-3-3epsilon associates with TAK1 in a phosphorylation-dependent manner to determine the cell fate of Bleomycin-treated HCC cells PMID: 23472066
  27. YWHAE gene duplication is associated with developmental, behavioral and brain abnormalities. PMID: 23813913
  28. 14-3-3epsilon may act as an important regulator in modulating tumor metastasis by promoting epithelial-mesenchymal transition as well as cell migration. PMID: 23483955
  29. The subcellular localization of full-length human MLF1 is 14-3-3epsilon-independent. PMID: 23271436
  30. The ERK/MAPK pathway is involved in the pathogenesis of gastric cancer; RKIP and 14-3-3epsilon exert an opposite effect on this pathway and the cells possibly via both direct and indirect reactions with the elements in this pathway. PMID: 22899242
  31. the -458G>T YWHAE variant contributes to the abnormal myocardial morphogenesis characteristic of LVNC as well as abnormal brain development, and implicate YWHAE as a novel candidate gene in pediatric cardiomyopathies. PMID: 23266643
  32. YWHAE gene contribute to the phenotype of small 17p13.3 chromosomal duplication in Miller-Dieker syndrome PMID: 23035971
  33. results suggest that YWHAE does play a major role in bipolar disorder in the Han Chinese population. PMID: 23140658
  34. These results showed that IgE is produced during malaria, and that the negative correlation between levels of IgE to 14-3-3 epsilon protein and parasitemia in asymptomatic malaria supports a role for these IgE molecules in defense mechanisms. PMID: 22984113
  35. Data obtained, which have never previously been reported, were used to modify the published 14-3-3 crystal structure using molecular modeling. PMID: 22580067
  36. When evaluating high-grade uterine sarcomas, cyclin D1 can be included in the immuno-histochemical panel as an indicator of YWHAE-FAM22endometrial stromal sarcoma. PMID: 22982899
  37. results indicate that the dosage effect of YWHAE varies from severe to very mild structural brain abnormalities, and suggest that the expression of YWHAE is associated with a complex mechanism of neuronal development PMID: 22887762
  38. 14-3-3epsilon is essential for the stable interaction of RIG-I with TRIM25, which facilitates RIG-I ubiquitination and initiation of innate immunity against hepatitis C virus and other pathogenic RNA viruses. PMID: 22607805
  39. Tumors with YWHAE-FAM22 rearrangements constitute a distinct group of endometrial stromal sarcoma (ESS), which is associated with high-grade morphology and aggressive clinical behavior compared to JAZF1 ESS. PMID: 22456610
  40. Collectively, these results identify a novel locus of apoptosomal regulation wherein MAPK signalling promotes Rsk-catalysed Apaf-1 phosphorylation and consequent binding of 14-3-3varepsilon, resulting in decreased cellular responsiveness to cytochrome c. PMID: 22246185
  41. Data present the high-resolution crystal structure of this binding motif [MLF1(29-42)pSer34] in complex with 14-3-3epsilon and analyse the interaction with isothermal titration calorimetry. PMID: 22151054
  42. Fluorescence in situ hybridization analysis demonstrated absolute specificity of YWHAE-FAM22A/B genetic rearrangement for high-grade endometrial stromal sarcoma. PMID: 22223660
  43. YWHAE does not play a major role in schizophrenia, major depressive disorder or bipolar disorder in the Han Chinese populatio PMID: 21184166
  44. Data suggest a regulatory circuitry involving Smad4 dependent up-regulation of KRT23 (directly or indirectly) which in turn modulates the interaction between KRT23 and 14-3-3epsilon leading to a cytoplasmic sequestration of 14-3-3epsilon. PMID: 21492476
  45. Results suggest that ERK and p38 MAPK regulate subcellular localization of Prdx6 by activation of 14-3-3epsilon as a chaperone protein, resulting in its translocation to acidic organelles. PMID: 21346153
  46. Overexpression of 14-3-3epsilon predicts tumour metastasis and poor survival in hepatocellular carcinoma PMID: 21401702
  47. For the first time, we found that TNF-alpha stimulation enhances the interactions between 14-3-3 epsilon and some key components in the mitogen-activated protein kinase (MAPK) signal module which is located at the immediate upstream of NF-kappaB. PMID: 20462248
  48. The 14-3-3 epsilon, zeta and theta may be involved in tumorigenesis of meningioma and be efficient markers for predicting the degree of malignancy in meningioma. PMID: 20388496
  49. Decreased expression of 14-3-3epsilon in contributes to the initiation and progression of laryngeal carcinoma by affecting apoptosis and invasion. PMID: 20565895
  50. the identification of a novel splicing variant of human 14-3-3 epsilon (14-3-3 epsilon sv), derived from a novel exon 1' insertion is reported. PMID: 20417184

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Subcellular Location
Nucleus. Cytoplasm. Melanosome.
Protein Families
14-3-3 family
Database Links

HGNC: 12851

OMIM: 605066

KEGG: hsa:7531

STRING: 9606.ENSP00000264335

UniGene: Hs.513851

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