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Western Blot
Positive WB detected in: HEK293T whole cell lysate(20µg), SH-SY5Y whole cell lysate(20µg), Jurkat whole cell lysate(20µg), NIH/3T3 whole cell lysate(20µg), A549 whole cell lysate(20µg), K562 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), HeLa whole cell lysate(20µg)
All lanes: YWHAZ antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time：30s
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Western Blot
Positive WB detected in: Mouse brain tissue lysate(20µg), Rat brain tissue lysate(20µg), Mouse uterus tissue lysate(20µg), Rat uterus tissue lysate(20µg)
All lanes: YWHAZ antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 27.7 kDa
Observed band size: 28 kDa
Exposure time: 2min
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Immunofluorescence staining of Hela cell with CSB-RA026293MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L).
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Immunofluorescence staining of MCF-7 cell with CSB-RA026293MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L).
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Overlay Peak curve showing A431 cells stained with CSB-RA026293MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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