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YWHAZ Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA026293MA1HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HEK293T whole cell lysate(20µg), SH-SY5Y whole cell lysate(20µg), Jurkat whole cell lysate(20µg), NIH/3T3 whole cell lysate(20µg), A549 whole cell lysate(20µg), K562 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), HeLa whole cell lysate(20µg)
    All lanes: YWHAZ antibody at 1:1000
    Secondary
    Goat polyclonal to human IgG at 1/40000 dilution
    Predicted band size: 28 kDa
    Observed band size: 28 kDa
    Exposure time：30s
  • Western Blot
    Positive WB detected in: Mouse brain tissue lysate(20µg), Rat brain tissue lysate(20µg), Mouse uterus tissue lysate(20µg), Rat uterus tissue lysate(20µg)
    All lanes: YWHAZ antibody at 1:1000
    Secondary
    Goat polyclonal to human IgG at 1/40000 dilution
    Predicted band size: 27.7 kDa
    Observed band size: 28 kDa
    Exposure time: 2min
  • Immunofluorescence staining of Hela cell with CSB-RA026293MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L).
  • Immunofluorescence staining of MCF-7 cell with CSB-RA026293MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L).
  • Overlay Peak curve showing A431 cells stained with CSB-RA026293MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P63104
Target Names
YWHAZ
Alternative Names
14 3 3 delta antibody; 14 3 3 protein zeta/delta antibody; 14 3 3 protein/cytosolic phospholipase A2 antibody; 14 3 3 zeta antibody; 14-3-3 protein zeta/delta antibody; 1433Z_HUMAN antibody; Epididymis luminal protein 4 antibody; Epididymis secretory protein Li 3 antibody; HEL S 3 antibody; HEL4 antibody; KCIP-1 antibody; KCIP1 antibody; MGC111427 antibody; MGC126532 antibody; MGC138156 antibody; Phospholipase A2 antibody; Protein kinase C inhibitor protein 1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; delta polypeptide antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta polypeptide antibody; Tyrosine 3/tryptophan 5 monooxygenase activation protein; zeta polypeptide antibody; YWHAD antibody; YWHAZ antibody
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant Human YWHAZ protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
19G7E9
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IF 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

YWHAZ, also known as 14-3-3 protein zeta/delta, belongs to the highly conserved 14-3-3 protein family that plays fundamental roles in signal transduction, cell cycle regulation, and apoptotic pathways. This adapter protein functions by binding to phosphoserine-containing motifs on target proteins, thereby modulating their activity, localization, and stability. Its involvement in diverse cellular processes including metabolism, protein trafficking, and stress responses makes YWHAZ a valuable target for researchers investigating cancer biology, neurodegenerative diseases, and cellular signaling networks.

This recombinant monoclonal antibody, clone 19G7E9, offers the consistency and reproducibility that demanding research applications require. Because recombinant antibodies are produced from defined sequences rather than hybridoma-derived sources, you can expect reliable lot-to-lot performance across long-term studies and collaborative projects. The antibody is affinity-purified and supplied in a glycerol-based buffer optimized for stability during storage.

Validation studies demonstrate robust performance across multiple experimental platforms. In Western blot applications, the antibody detects a clean band at the expected 28 kDa molecular weight across an extensive panel of human cell lines including HEK293T, SH-SY5Y, Jurkat, A549, K562, PC-3, MCF-7, and HeLa cells. Cross-species reactivity has been confirmed through successful detection in mouse and rat brain and uterus tissue lysates, expanding its utility for comparative and translational studies. Immunofluorescence staining has been validated in HeLa and MCF-7 cells, revealing cytoplasmic localization patterns consistent with YWHAZ's known cellular distribution. Flow cytometry applications have been demonstrated using A431 cells with clear separation from isotype controls.

This versatile antibody supports researchers exploring 14-3-3 protein biology, signal transduction mechanisms, and disease-relevant pathways across human and rodent model systems.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity.
Gene References into Functions
  1. Amount of 14-3-3 proteins is decreased in pineal gland, blood platelets and ileum of patients with ASD. PMID: 28522826
  2. These results imply that disorder in the N-terminal helices of 14-3-3 zeta is a consequence of the dimer-monomer dynamics and may play a role in conferring chaperone function to 14-3-3 zeta protein. PMID: 29109150
  3. Knockdown of YWHAZ inhibited cell cycle progression, migration, and the expression of stem cell markers and tumorigenicity was suppressed in tumor-bearing BALB/c nude mice. The expression of YWHAZ was directly down-regulated by miR-30e in resistant ovarian cancer cells. PMID: 30134224
  4. our data suggest miR-204 and 14-3-3zeta as potential therapeutic targets in osteosarcoma PMID: 29441884
  5. evidence is lacking to conclude that 14-3-3zeta is a useful marker of tamoxifen resistance. PMID: 28643021
  6. TRIM21 positively regulated osteosarcoma cell proliferation. Overexpression of TRIM21 enhanced osteosarcoma cell tolerance toward various stresses. YWHAZ protein was identified as a novel interacting partner of TRIM21 and its expression levels were negatively regulated by TRIM21. PMID: 29673441
  7. several disordered regions of PI4KB become protected from proteolytical degradation upon 14-3-3 binding. PMID: 28864297
  8. Ectopic expression of miR-451 could inhibit the cell migration and invasion, promoted apoptosis, induced cell-cycle arrest Furthermore, tyrosine3-monooxygenase/tryptophan5-monooxygenase activation protein zeta (YWHAZ) was identified as a direct target of miR-451 PMID: 28981108
  9. Serum autoantibodies to YWHAZ are produced at substantially greater levels in gastric cancer patients as compared to controls. PMID: 28944820
  10. Dimerization of 14-3-3 zeta (14-3-3zeta) dimer was disrupted by a double mutant (L12E, M78K). PMID: 29203375
  11. Results identified YWHAZ as the direct target of miR-613 in hepatocellular carcinoma (HCC). Its overexpression reverses the tumor suppressing role of miR-613 in HCC cells. PMID: 29551505
  12. 14-3-3zetaoverexpression might be a potential prognostic biomarker for ovarian cancer. PMID: 29214776
  13. In AML patients, low level of miR-451 is negatively correlated with high levels of c-Myc and YWHAZ, while c-Myc level is positively related to YWHAZ expression. These results suggested that c-Myc dash, verticalmiR-451 dash, verticalYWHAZ/AKT cascade might play a crucial role during leukemogenesis, and reintroduction of miR-451 could be as a potential strategy for AML therapy. PMID: 27764807
  14. miR-22 exhibits tumor-suppressive effects in hepatocellular carcinoma cells by regulating YWHAZ/AKT/FOXO3a signaling. PMID: 27811373
  15. our data demonstrate that overexpression of 14-3-3zeta in early stage pre-cancerous breast epithelial cells may trigger an elevated glycolysis and transcriptionally up-regulating LDHA, thereby contributes to human breast cancer initiation. PMID: 27150057
  16. 14-3-3zeta can bind to the FOXO3a transcription factor to promote the export of the complex to the cytoplasm, leading to enhanced proliferation and migration of tongue cancer cells. PMID: 27080223
  17. Structure of the complex of phosphorylated liver kinase B1 and 14-3-3zeta has been reported. PMID: 28368277
  18. These results suggest that the hypoxia/14-3-3zeta/HIF-1alpha pathway plays an important role in portal vein tumor thrombus formation and hepatocellular carcinoma metastasis PMID: 26910835
  19. 14-3-3zeta recruited YAP and p-LATS to form a complex under high cells density status and 14-3-3zeta other than YAP or phospho-LATS was the key regulatory molecule of this complex. PMID: 27334574
  20. This study shows that human procaspase-2 interaction with 14-3-3 zeta is governed by phosphorylation at both S139 and S164. PMID: 28943433
  21. The results highlight a new role of TSC2 in protecting glioblastoma against photodynamic therapy-induced cell death, and TSC2 and YWHAZ as new RIP3 partners. PMID: 27984090
  22. These results suggest that 14-3-3-zeta is involved in the TLR3-TICAM-1 pathway in promoting multimerization of TICAM-1 for the formation of a TICAM-1 signalosome. PMID: 27058640
  23. The data indicate that microtubule-bound tau is resistant to 14-3-3zeta-induced tau aggregation and suggest that tau phosphorylation promotes tau aggregation in the brain by detaching tau from microtubules and thus making it accessible to 14-3-3zeta. PMID: 27548710
  24. Structural interface between LRRK2 and 14-3-3 delta protein has been presented. PMID: 28202711
  25. 14-3-3zeta-mediated invasion of cancer cells was found to upregulate Snail through the activation of atypical protein kinase C (aPKC). PMID: 27554601
  26. results have identified a novel mechanism by which 14-3-3sigma maintains the epithelial phenotype by inhibiting Epithelial to Mesenchymal Transition and suggest that this property of 14-3-3sigma might contribute to its function as a tumor suppressor gene. PMID: 27261462
  27. 14-3-3zeta regulates HIF-1alpha production in hepatocellular carcinoma cells by directly binding to HIF-1alpha and via PI3K/Akt/NF-small ka, CyrillicB signal transduction pathway. PMID: 26884855
  28. Results indicate that HuR induces 14-3-3zeta translation via interaction with its 3' UTR and that 14-3-3zeta is necessary for stimulation of intestinal epithelial cell migration after wounding. PMID: 27401462
  29. this study suggests that the down-regulation of 14-3-3 zeta leads to the inhibition of TGFb1- induced contraction by decreasing the expression of total RhoA in TM cells. PMID: 26906158
  30. loss of expression or even the down-regulation of c-abl, but not WYHAZ, is a fundamental event that leads to genesis and progression of tumors PMID: 26429164
  31. This study provides the molecular basis for C-Raf C-terminal-derived phosphopeptide interaction with 14-3-3zeta protein and gives structural insights responsible for phosphorylation-mediated protein binding. PMID: 26295714
  32. 14-3-3z may play an important role in signaling pathway in breast cancer. Also, a high 14-3-3z expression could positively regulate growth factor receptors and protein kinase pathways PMID: 25861752
  33. Studies show that 14-3-3zeta is overexpressed in oral squamous cell carcinoma and provide evidence that may regulate tumor inflammation and immune response through Stat3 signaling. PMID: 25556369
  34. Activation of PCTAIRE-1 is mediated through interaction with the phosphorylated form of cyclin Y in complex with 14-3-3. PMID: 26205494
  35. C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer. PMID: 25971962
  36. Our findings indicate that YWHAZ could serve as a promising prognostic biomarker in localized PCa to predict poor prognosis PMID: 25156059
  37. studyconfirmed the interaction of Ser9-phosphorylated GSK3beta with 14-3-3zeta; Ser9-phosphorylation of GSK3beta promoted by 14-3-3zeta is critical for the activation of NF-kappaB pathway PMID: 25138042
  38. A detailed analysis of the interaction between singly or doubly phosphorylated human tyrosine hydroxylase isoform 1(1-50) peptides and 14-3-3zeta PMID: 25418103
  39. BIS targeting induces cellular senescence through the regulation of 14-3-3 zeta/STAT3/SKP2/p27 in glioblastoma cells. PMID: 25412315
  40. Aberrant upregulation of 14-3-3sigma and EZH2 expression serves as an inferior prognostic biomarker for hepatocellular carcinoma. PMID: 25226601
  41. The 14-3-3zeta-driven contextual changes of Smad partners from p53 to Gli2 may serve as biomarkers and therapeutic targets of TGF-b-mediated cancer progression. PMID: 25670079
  42. Among the genes found disrupted in this study, there is evidence suggesting that YWHAZ and also the X-linked DRP2 may be considered as novel autism candidate genes. PMID: 23999528
  43. Data found that the interaction between 14-3-3 zeta and Atg9A is mediated by phosphorylation at Ser761. PMID: 25266655
  44. miR-375-mediated regulation of 14-3-3zeta contributes to decrease telomerase activity by altering nuclear translocation of TERT. PMID: 24708873
  45. 14-3-3zeta regulates nuclear trafficking of PP1alpha in mammalian cells PMID: 24956593
  46. By preventing the inactivation of cofilin, metabolic stress-induced degradation of 14-3-3zeta promotes the conversion of blood monocytes into a hypermigratory, proatherogenic phenotype. PMID: 24812321
  47. Compared to HL-60 cells, multidrug-resistant HL-60/VCR cells had increased 14-3-3zeta mRNA and protein expression.Silencing of 14-3-3zeta increased the sensitivity of both sensitive and resistant HL-60 cells to TPT-induced apoptosis. PMID: 24603438
  48. 14-3-3zeta causes synaptic loss by destabilizing microtubules, leading to proteosomal degradation of synaptophysin in the neurons of patients suffering from Alzheimer's disease. PMID: 24367683
  49. Data suggest that the combined expression of 14-3-3zeta and Hsp27 may be a biomarker for predicting survival in patients with NSCLC, and this combination may have potential as a therapeutic target for NSCLC. PMID: 24804299
  50. Somatic copy number alterations by whole-exome sequencing implicates YWHAZ and PTK2 in castration-resistant prostate cancer. PMID: 24114522

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Subcellular Location
Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.
Protein Families
14-3-3 family
Database Links

HGNC: 12855

OMIM: 601288

KEGG: hsa:7534

STRING: 9606.ENSP00000309503

UniGene: Hs.492407

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