Product Details

Uniprot No.

Target Names

YY1

Alternative Names

CF1 antibody; Delta antibody; Delta transcription factor antibody; INO80 complex subunit S antibody; INO80S antibody; NF E1 antibody; NF-E1 antibody; NFE1 antibody; OTTHUMP00000197459 antibody; Transcriptional repressor protein YY1 antibody; TYY1_HUMAN antibody; UCR motif DNA binding protein antibody; UCRBP antibody; Yin and yang 1 antibody; Yin and Yang 1 protein antibody; Yin Yang 1 antibody; Ying Yang 1 antibody; YY 1 antibody; YY 1 transcription factor antibody; YY-1 antibody; YY1 antibody; YY1 transcription factor antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human YY1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3A11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the YY1 recombinant monoclonal antibody involves the application of DNA recombinant technology and in vitro genetic manipulation. Firstly, animals are immunized with a synthesized peptide derived from human YY1, leading to the isolation and selection of positive B cells. Subsequently, the identified positive B cells undergo single clone identification to ensure their specificity. The light and heavy chains of the YY1 antibody are then amplified through PCR and inserted into a plasmid vector. This recombinant vector is introduced into a host cell line, allowing for the expression of the antibody. The YY1 recombinant monoclonal antibody is purified from the cell culture supernatant utilizing affinity chromatography. It exhibits strong binding to human YY1 protein and is well-suited for ELISA.

The YY1 protein is a transcription factor that can bind to DNA and regulate the expression of genes involved in various cellular processes, such as cell proliferation, differentiation, and apoptosis. YY1 can also function as a repressor, depending on the specific context and interacting partners. One of the main functions of YY1 is to regulate the balance between cell proliferation and cell death. YY1 also plays a role in DNA damage response by regulating the expression of genes involved in cell cycle checkpoint control and DNA repair. In addition, YY1 is involved in the regulation of immune responses, inflammation, and metabolism.

Target Background

Function

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation.; Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.

Gene References into Functions

Data show that MiR-147b targets the 3' untranslated region (3'UTR) of Yin Yang 1 (YY1). PMID: 30060075
Results show that YY1 is phosphorylated by c-Abl kinase at tyrosine residue 254 and that this phosphorylation likely modulates its transcriptional activity. PMID: 29807053
YY1-CDKN3-MDM2/P53-P21 axis is involved in pancreatic tumorigenesis PMID: 29168185
The repression of TBX21 expression by high-affinity binding of YY1 to the -1993C allele may contribute to a decreased development of AIH-1 via suppression of type 1 immunity. PMID: 29358858
expression of miR-192-5p was negatively correlated with that of YY1 in bladder cancer tissues PMID: 29536411
YY1 promoted glioma cell proliferation and miR218 could inhibit glioma cell proliferation by targeting YY1. PMID: 29138857
miR-30a acts in a feedback loop to modulate the pro-autophagic activities of YY1. PMID: 29052509
Data suggest a role for YY1 transcription factor (YY1) in transcriptional activity on inactive X (Xi) in general through sequence-specific binding, and its involvement at superloop anchors. PMID: 27857184
Results suggest that up-regulation of YY1 is closely associated with the progression of cervical squamous cell carcinoma, and YY1 may play an important role in the pathogenesis of cervical cancer by modulating the expression of E-cadherin and HPV16 E6. PMID: 29470526
Results show that histone deacetylase 1 (HDAC1) expression was positively correlated with YY1 transcription factor (YY1) in hepatocellular carcinoma (HCC) cell lines and primary tumor tissues. PMID: 28489564
Authors found that YY1 and STAT1 were upregulated in ox-LDL-stimulating macrophages followed by translocation in the nucleus and binding to the transcriptional promoter region of miR-29a, thus leading to the increase of miR-29a expression. PMID: 28593745
results support a model in which YY1 acts as an architectural protein to connect developmentally regulated looping interactions; the location of YY1-mediated interactions may be demarcated in development by a preexisting topological framework created by constitutive CTCF-mediated interactions. PMID: 28536180
High expression of nuclear YY1 was significantly associated with higher AJCC stage and small vessel invasion. PMID: 28412749
This is the first report on the status of YY1 T372R in insulinoma cases of Indian origin. PMID: 28472826
YY1 promotes proliferation and migration with suppression of apoptosis via directly inhibiting MYCT1 in laryngeal cancer cells, suggesting that YY1 is a useful target as a potential oncogene in laryngeal cancer development and progression. PMID: 28485541
These data suggested that YY1 may be important for apoptosis induction via the regulation of Fas during sepsis. Therefore, Fas may be a potential therapeutic target to prevent MOD through regulation of YY1 expression. Furthermore, YY1 and Fas expression in PBMCs may be used to as prognostic markers. PMID: 28447715
Study shows that the ubiquitously expressed transcription factor Yin Yang 1 (YY1) contributes to enhancer-promoter structural interactions in a manner analogous to DNA interactions mediated by CTCF. PMID: 29224777
YY1 was over-expressed in RA which can bind to the promotor of IL-6 to introduce IL-6 expression, contributing to the inflammation of RA via stimulation of Th17 differentiation. PMID: 27829535
YY1 promotes apoptosis via upregulating Bax transcription and subsequent activation of Bax by translocation from the cytosol to the mitochondrial membrane. PMID: 27074573
High YY1 expression is associated with Lung Cancer Progression. PMID: 28972861
Low YY1 expression is associated with pancreatic ductal adenocarcinoma. PMID: 28867247
PRE-PIK3C2B interacts directly with YY1 in vitro and recruits PRC2 complex in vivo. The localization of PcG proteins including YY1 to PRE-PIK3C2B in HEK cells is decreased on knock-down of either YY1 or SUZ12. PMID: 23805300
YY1 promotes mTORC2-mediated AKT activation PMID: 26762111
these results define a clinical syndrome caused by haploinsufficiency of YY1 through dysregulation of key transcriptional regulators. PMID: 28575647
Interactions of the chromatin remodeling protein INO80 with DNA, and the role of YY1 in this process have been described. PMID: 27428271
ZFs of YY1 are able to interact with multiple RNA sequences, and that their specificity is not limited to putative endogenous RNA target Xist. The mode of interaction appears to be distinct from both the DNA binding of YY1 and the RNA binding of other classical ZF proteins. PMID: 27369384
the existence of both INO80 and YY1 is required for recruiting the INO80/YY1 complex to BCCIP promoter region. Our findings strongly indicate that BCCIP is a potential target gene of the INO80/YY1 complex. PMID: 27535137
miR-215 is downregulated in colon cancer and it suppresses colon cancer cell proliferation, migration and invasion by directly targeting YY1 PMID: 27663660
High YY1 expression is Associated with Endometrial Cancer. PMID: 27259051
Our results suggested that YY1 depletion repressed prostate cancer cell viability and proliferation and induced apoptosis at least in a miR-146a-assisted manner. PMID: 28101571
Knockdown of miR-381 target gene, YY1, mimicked the phenotype induced by miR-381 overexpression. PMID: 26768613
SET7/SET9-mediated YY1 methylation was shown to be involved in YY1-regulated gene transcription and cell proliferation. PMID: 26902152
The results of our study indicate that YY1 belongs to the family of intrinsically disordered proteins, which exist natively in a partially unfolded conformation. PMID: 25963536
Study shows that YY1 regulates inflammatory responses in vascular endothelium through its binding to ANRIL. PMID: 26618242
YY1 regulates lung CCL4 transcription in pulmonary tuberculosis. PMID: 26786659
Significance of the pathogenic mutation T372R in the Yin Yang 1 protein interaction with DNA--thermodynamic studies: the mutation does not affect the secondary structure of either zinc fingers or the N-terminal fragment of the protein. PMID: 26910132
YY1 overexpression contributes to EZH2 recruitment for H3K27me3-mediated silencing of tumour-suppressive microRNAs, thereby activating NF-kappaB signalling in hepatocarcinogenesis. PMID: 26800240
IL-18 induced YY1 expression, which repressed FXR and MRP2 expression in HepG2 cells. PMID: 26292095
YY1 is overexpressed in differentiated thyroid cancer, and more frequently overexpressed in the papillary carcinoma subtype. PMID: 25698133
Results identify a novel YY1 ~ miR-9 ~ RYBP axis involved in melanoma tumorigenesis and reinforce the idea that regulatory circuitries involving miRNAs and TFs are prevalent mechanisms. PMID: 26104682
Rescue experiments indicated that the suppressive effect of miR-34a on invasion and migration was mediated by activating YY1 expression. PMID: 25954903
Results demonstrate that YY1 is a critical effecter in IL-13-induced fibroblast differentiation: increasing YY1 enhances it whereas decreasing YY1 reduces it. PMID: 25775215
These findings imply that the T allele significantly suppresses PSCA expression in vivo by recruiting YY1 to its promoter, which eventually predisposes gastric epithelial cells to GC development PMID: 25727947
Data show that YY1 transcription factor (YY1) is tyrosine-phosphorylated by Src family kinases, leading to down-regulation of its activity. PMID: 26198631
YY1 is a transcription repressor of FEN1 regulating FEN1 levels in response to DNA damaging agents. PMID: 25885449
These data demonstrate the crucial requirement for YY1 in lung morphogenesis. PMID: 26329601
Transcription factor YY1 is critically involved in the chromatin remodeling and chromosomal interactions at the Th2 cytokine locus. PMID: 25764217
The somatic Thr372Arg YY1 mutation is a relevant finding in female patients with sporadic insulinomas. PMID: 25763608
YY1 is necessary to maintain the steady-state level of VEGFA expression while its silencing increases VEGFA mRNA half-life at 4 h and impairs the maturation of miRNA 200b/c. PMID: 25644713
YY1 regulates, in a sex-specific manner, Mena expression in heart failure PMID: 25935483