Code | CSB-MA000011M0m |
Size | US$120 |
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Application | Recommended Dilution |
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WB | 1:500-1:2000 |
The 6His Monoclonal Antibody specifically reacts to recombinant proteins containing the 6xHis-Tag. The 6*His tag is widely used in recombinant protein isolation and purification, and this antibody is a valuable tool for researchers who need to detect or isolate proteins that have been tagged with the 6*His sequence. This antibody is immunized with a 6*His synthetic peptide conjugated to KLH and produced in mouse.
The 6*His Monoclonal Antibody underwent protein A mediated purification and was purified to a high degree of purity, >95%. It is available in liquid form and belongs to the IgG2a isotype. This antibody has been validated in both ELISA and WB assays, making it a useful tool for the detection and quantification of recombinant proteins containing the 6*His-Tag. Its high specificity and purity ensure reliable and reproducible results. This antibody has been cited in a paper by Dominguez G.et al. in 2018, increasing its reliability and usefulness.
Applications : WB
Sample type: Aliquots of the cultures
Review: Validation of anti-SphK2 antibody. Escherichia coli strain BL21 overexpressing His-tagged full length SphK1, His-tagged full length SphK2, and human brain lysate were analyzed by Western blotting using anti-Histidine.
By Anonymous