6*His Monoclonal Antibody

Datasheet
Code CSB-MA000011M0m
Product Type Monoclonal Antibody
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • All lanes :Mouse Anti-6*his monoclonal antibody at 1μg/ml
    Western blot analysis of 6*his Epitope Tag was performde by loading various amounts of lysates containing different HIS tagged
    Recombinant proteins per well onto a 15% Tris-HCL polyacrylamide gel.
    Line 1: FABP3 insect cell lysate
    Line 2: TK1 insect cell lysate
    Line 3: cagA insect cell lysate
    Line 4: NGAL insect cell lysate
    Line 5: IGFBP1 293T cell lysate
    Line 6: TRY2 293T cell lysate

  • All lanes : Recombinant DST Protein at 30ng/ml
    Line 1: Mouse Anti-his monoclonal antibody at 1:64000
    Line 2: Mouse Anti-his monoclonal antibody at 1:32000
    Line 3: Mouse Anti-his monoclonal antibody at 1:16000
    Line 4: Mouse Anti-his monoclonal antibody at 1:8000
    Line 5: Mouse Anti-his monoclonal antibody at 1:4000
    Observed band size : 35kd
    Predicted band size : 35kd

Immunogen HHHHHH(6x His) synthetic peptide conjugate to KLH
Raised in Mouse
Tested Applications ELISA, WB; Recommended dilution: WB:1:500-1:5000
Relevance A HIS tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the HIS codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%,Protein G purified
Isotype IgG2a
Clonality Monoclonal
Alias 6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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