6*His Monoclonal Antibody

Code CSB-MA000011M0m
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
    All lanes: HIS antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 55 kDa
    Observed band size: 55 kDa
     

  • Western Blot
    Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
    All lanes: HIS antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 60 kDa
    Observed band size: 60 kDa

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Product Details

Description
6*His monoclonal antibody CSB-MA000011M0m was produced in mouse immunized by using HHHHHH(6x His) synthetic peptide conjugate to KLH as the immunogen. The immunogen, 6x His, a short peptide, consists of six histidine residues (His-His-His-His-His-His). It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
This 6*His Monoclonal Antibody was tested in the WB, IHC, ICC and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.
Target Names 6*His
Alternative Names 6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
Raised in Mouse
Species Reactivity N/A
Immunogen HHHHHH(6x His) synthetic peptide conjugate to KLH
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG2a
Clone No. 3G5H8
Purification Method >95%, Protein A purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain. Dominguez G.et al,Acta Neuropathol Commun,2018

Sample type: Aliquots of the cultures
Application: WB
Review: Validation of anti-SphK2 antibody. Escherichia coli strain BL21 overexpressing His-tagged full length SphK1, His-tagged full length SphK2, and human brain lysate were analyzed by Western blotting using anti-Histidine.
PMID: 29615132

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