6*His Monoclonal Antibody

Datasheet
Code CSB-MA000011M0m
Product Type Monoclonal Antibody
Size US$200Purchase it in Cusabio online store
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Image
  • Western Blot
    Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
    All lanes: HIS antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 55 kDa
    Observed band size: 55 kDa
     

  • Western Blot
    Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
    All lanes: HIS antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 60 kDa
    Observed band size: 60 kDa

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen HHHHHH(6x His) synthetic peptide conjugate to KLH
Raised in Mouse
Tested Applications ELISA, WB; Recommended dilution: WB:1:500-1:2000
Relevance A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein A purified
Isotype IgG2a
Clonality Monoclonal
Alias 6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 3G5H8
References
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