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| Code | CSB-MA000011M0m |
| Size | US$200 |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The 6His Monoclonal Antibody specifically reacts to recombinant proteins containing the 6xHis-Tag. The 6*His tag is widely used in recombinant protein isolation and purification, and this antibody is a valuable tool for researchers who need to detect or isolate proteins that have been tagged with the 6*His sequence. This antibody is immunized with a 6*His synthetic peptide conjugated to KLH and produced in mouse. The 6*His Monoclonal Antibody underwent protein A mediated purification and was purified to a high degree of purity, >95%. It is available in liquid form and belongs to the IgG2a isotype. This antibody has been validated in both ELISA and WB assays, making it a useful tool for the detection and quantification of recombinant proteins containing the 6*His-Tag. Its high specificity and purity ensure reliable and reproducible results. This antibody has been cited in a paper by Dominguez G.et al. in 2018, increasing its reliability and usefulness. |
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| Target Names | 6*His | ||||
| Alternative Names |
6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
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| Raised in | Mouse | ||||
| Species Reactivity | N/A | ||||
| Immunogen | HHHHHH(6x His) synthetic peptide conjugate to KLH | ||||
| Conjugate | Non-conjugated | ||||
| Clonality | Monoclonal | ||||
| Isotype | IgG2a | ||||
| Clone No. | 3G5H8 | ||||
| Purification Method | >95%, Protein A purified | ||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
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| Form | Liquid | ||||
| Tested Applications | ELISA, WB | ||||
| Recommended Dilution |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
Applications : WB
Sample type: Aliquots of the cultures
Review: Validation of anti-SphK2 antibody. Escherichia coli strain BL21 overexpressing His-tagged full length SphK1, His-tagged full length SphK2, and human brain lysate were analyzed by Western blotting using anti-Histidine.
By Anonymous
