6*His Monoclonal Antibody

Code CSB-MA000011M0m
Size US$120
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  • Western Blot
    Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
    All lanes: HIS antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 55 kDa
    Observed band size: 55 kDa
     

  • Western Blot
    Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
    All lanes: HIS antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 60 kDa
    Observed band size: 60 kDa

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Product Details

Target Names
6*His
Alternative Names
6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
Raised in
Mouse
Species Reactivity
N/A
Immunogen
HHHHHH(6x His) synthetic peptide conjugate to KLH
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
IgG2a
Clone No.
3G5H8
Purification Method
>95%, Protein A purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The 6His Monoclonal Antibody specifically reacts to recombinant proteins containing the 6xHis-Tag. The 6*His tag is widely used in recombinant protein isolation and purification, and this antibody is a valuable tool for researchers who need to detect or isolate proteins that have been tagged with the 6*His sequence. This antibody is immunized with a 6*His synthetic peptide conjugated to KLH and produced in mouse.

The 6*His Monoclonal Antibody underwent protein A mediated purification and was purified to a high degree of purity, >95%. It is available in liquid form and belongs to the IgG2a isotype. This antibody has been validated in both ELISA and WB assays, making it a useful tool for the detection and quantification of recombinant proteins containing the 6*His-Tag. Its high specificity and purity ensure reliable and reproducible results. This antibody has been cited in a paper by Dominguez G.et al. in 2018, increasing its reliability and usefulness.

Customer Reviews and Q&A

 Customer Reviews
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Applications : WB

Sample type: Aliquots of the cultures

Review: Validation of anti-SphK2 antibody. Escherichia coli strain BL21 overexpressing His-tagged full length SphK1, His-tagged full length SphK2, and human brain lysate were analyzed by Western blotting using anti-Histidine.

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