ACTB Monoclonal Antibody

Datasheet

Code	CSB-MA000091M2m
Size	US$120
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue, Rat heart tissue, Rat skeletal muscle tissue, Rabbit lung tissue, Rabbit liver tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time：5min Western Blot Positive WB detected in: 293T whole cell lysate, Zebrafish tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time：5min Western Blot Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse skeletal muscle tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time：5min Western Blot Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue, U87 whole cell lysate, Rat skeletal muscle tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time：5min Western Blot Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue, A549 whole cell lysate, MCF-7 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time：5min Western Blot Positive WB detected in: Hela whole cell lysate at 40μg, 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time：5min Western Blot Positive WB detected in: 20μg Hela whole cell lysate ACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time：5min
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Product Details
Customer Reviews and Q&A

Product Details

Target Names

ACTB

Alternative Names

ACTB; ACTB; ACTB; PS1TP5BP1; beta-ac6n; β-ac6n

Raised in

Mouse

Species Reactivity

Human, Mouse, Rat, Rabbit

Immunogen

A synthesized peptide derived from human Beta-Actin (1-50AA)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

6D8D8

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:10000-1:1280000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Beta-actin (ACTB) is a constitutively expressed cytoskeletal housekeeping protein widely adopted as a standard endogenous loading control for normalizing protein expression data in biochemical research. CSB-MA000091M2m is a mouse monoclonal IgG1 antibody generated against a synthetic peptide from human ACTB (residues 1–50), purified via Protein G affinity chromatography with purity above 95% to guarantee consistent batch-to-batch performance and low non-specific background. Designed as a reliable loading control antibody, it demonstrates broad cross-species reactivity covering human, mouse, rat, and rabbit samples. Western blot detects a distinct 42 kDa target band across diverse cell lysates and multiple organ tissues, delivering clear signals at an ultra-high dilution up to 1:1280000 and reliably detecting lysate loads as low as 0.3125 μg. Validated for ELISA and Western blot applications, this antibody provides flexible working dilution ranges and delivers stable, reproducible results, serving as a dependable reference standard for quantitative proteomics and cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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