ACTB Monoclonal Antibody

Code CSB-MA000091M2m
Size US$120
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  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue, Rat heart tissue, Rat skeletal muscle tissue, Rabbit lung tissue, Rabbit liver tissue
    All lanes: ACTB antibody at 1:5000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 42 KDa
    Observed band size: 42 KDa
    Exposure time:5min
  • Western Blot
    Positive WB detected in: 293T whole cell lysate, Zebrafish tissue
    All lanes: ACTB antibody at 1:5000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 42 KDa
    Observed band size: 42 KDa
    Exposure time:5min
  • Western Blot
    Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse skeletal muscle tissue
    All lanes: ACTB antibody at 1:5000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 42 KDa
    Observed band size: 42 KDa
    Exposure time:5min
  • Western Blot
    Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue, U87 whole cell lysate Rat skeletal muscle tissue
    All lanes: ACTB antibody at 1:5000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 42 KDa
    Observed band size: 42 KDa
    Exposure time:5min
  • Western Blot
    Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue, A549 whole cell lysate, MCF-7 whole cell lysate
    All lanes: ACTB antibody at 1:5000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 42 KDa
    Observed band size: 42 KDa
    Exposure time:5min
  • Western Blot
    Positive WB detected in: Hela whole cell lysate at 40μg, 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg
    All lanes: ACTB antibody at 1:5000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 42 KDa
    Observed band size: 42 KDa
    Exposure time:5min
  • Western Blot
    Positive WB detected in: 20μg Hela whole cell lysate
    ACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 42 KDa
    Observed band size: 42 KDa
    Exposure time:5min
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Product Details

Target Names
ACTB
Alternative Names
ACTB;ACTB;ACTB;PS1TP5BP1;beta-ac6n;β-ac6n
Raised in
Mouse
Species Reactivity
Human, Mouse, Rat, Rabbit
Immunogen
A synthesized peptide derived from human Beta-Actin (1-50AA)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
IgG1
Clone No.
6D8D8
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:10000-1:1280000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The ACTB monoclonal antibody is created using the synthesized peptide derived from human Beta-Actin (1-50aa) to immunize the mouse, with hybridoma cells being generated by fusing mouse B cells (isolated from the immunized mouse) with myeloma cells. The hybridoma cells that produce the ACTB antibody are then selected and cultured, and the ACTB monoclonal antibody is purified from the mouse ascites using protein G affinity chromatography. This unconjugated IgG1 antibody is highly specific to the ACTB proteins from four species including human, mouse, rat, and rabbit. It can be used in ELISA and WB assays.

ACTB, also known as beta-actin, is a protein that plays a structural role in the cytoskeleton of eukaryotic cells. It is involved in maintaining cell shape, adhesion, and motility, and is also important for intracellular transport and cytokinesis. Additionally, ACTB is involved in gene transcription and chromatin remodeling and has been shown to interact with a variety of proteins involved in signaling pathways and cellular processes.

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