Avi-Tag Monoclonal Antibody

The Avi-Tag is a widely utilized 15-amino acid peptide sequence that serves as a specific substrate for the bacterial biotin ligase, BirA. This system enables precise, site-specific enzymatic biotinylation of target proteins in vivo or in vitro. The resulting biotinylated proteins can then be efficiently captured or detected using streptavidin or avidin-based systems, making Avi-Tag an invaluable tool for protein engineering, affinity purification, and molecular interaction studies.

The Mouse Anti-Avi-Tag Monoclonal Antibody (CSB-MA000314, IgG isotype) is specifically developed to provide high-affinity recognition of this unique peptide sequence. Produced via hybridoma technology using a recombinant Avi-tagged protein as the immunogen, this antibody ensures exceptional specificity and lot-to-lot consistency, with little cross-reactivity to endogenous biotin.

Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity and robust immunoreactivity. As demonstrated in the Western Blot analysis, the antibody successfully detects Avi-tagged recombinant protein at two different working dilutions: 1:5,000 and 1:10,000. The clear, crisp signals observed at both concentrations confirm its reliability for precise molecular weight identification and highlight its excellent signal-to-noise ratio, making it a highly cost-effective reagent for routine laboratory use.

This antibody is an indispensable tool for molecular biologists working with tagged constructs. It enables reliable confirmation of successful protein expression, accurate assessment of protein molecular weight, and serves as a critical reagent for downstream purification and detection workflows.