E-Tag Monoclonal Antibody

Code CSB-MA000151M0m
Size Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
    All lanes: E-Tag antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36 kDa
     

  • Western Blot
    Positive WB detected in: E-tagged fusion protein
    All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36 kDa
     

  • Immunoprecipitating E-Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
    Lane 3: 293F transfected whole cell lysate (20µg)

     

  • ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
    Antigen at dilution of 1µg/ml
    E-Tag antibody at (0-0.15µg)
    Goat polyclonal to Mouse IgG at 1/50000 dilution

     

  • Western Blot
    Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
    All lanes: E-Tag antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 30, 68, 55, 67-157kDa
    Observed band size: 30, 68, 55, 67-157kDa

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Product Details

Description

The monoclonal anti-E-Tag (mouse IgG1 isotype) is produced from the hybridoma fused by the mouse myeloma cells and splenocytes from the immunized mouse. The splenocytes are isolated from the mouse immunized with the E-tag synthetic peptide conjugated to KLH and can secret the E-Tag antibody. This unconjugated E-Tag antibody is purified through protein G with a purity of more than 95%. It only reacts with samples containing E-Tag. And it has been amenable for ELISA, WB, and IP applications.

The E-tag is a popular epitope tag containing 13 amino acids. It is commonly expressed or detected with the protein of interest as an N- or C-terminal fusion. Anti-E tag antibodies are amenable for the detection, isolation, and purification of E-tagged proteins.

Target Names E-Tag
Alternative Names E-Tag
Raised in Mouse
Species Reactivity All
Immunogen GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
Conjugate Non-conjugated
Clonality Monoclonal Antibody
Isotype IgG1
Clone No. 11H12B3
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IP
Recommended Dilution
Application Recommended Dilution
ELISA 1:4000-1:8000
WB 1:1000-1:320000
IP 1μg-5μg
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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