E-Tag Monoclonal Antibody

Datasheet
Code CSB-MA000151M0m
Product Type Monoclonal Antibody
Size
Image
  • Western Blot
    Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
    All lanes: E-Tag antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36 kDa
     

  • Western Blot
    Positive WB detected in: E-tagged fusion protein
    All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36 kDa
     

  • Immunoprecipitating E-Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
    Lane 3: 293F transfected whole cell lysate (20µg)

     

  • ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
    Antigen at dilution of 1µg/ml
    E-Tag antibody at (0-0.15µg)
    Goat polyclonal to Mouse IgG at 1/50000 dilution

     

  • Western Blot
    Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
    All lanes: E-Tag antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 30, 68, 55, 67-157kDa
    Observed band size: 30, 68, 55, 67-157kDa

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Immunogen GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
Raised in Mouse
Species Reactivity All
Tested Applications ELISA, WB, IP; Recommended dilution: ELISA:1:4000-1:8000 WB:1:1000-1:320000, IP: 1µg-5µg
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG1
Clonality Monoclonal Antibody
Alias E-Tag
Target Names E-Tag
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 11H12B3
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