E-Tag Monoclonal Antibody

Datasheet

Code	CSB-MA000151M0m
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Image	Western Blot Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: E-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Western Blot Positive WB detected in: E-tagged fusion protein All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Immunoprecipitating E-Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg) Lane 3: 293F transfected whole cell lysate (20µg) ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag) Antigen at dilution of 1µg/ml E-Tag antibody at (0-0.15µg) Goat polyclonal to Mouse IgG at 1/50000 dilution Western Blot Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag All lanes: E-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 30, 68, 55, 67-157kDa Observed band size: 30, 68, 55, 67-157kDa
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Product Details
Customer Reviews and Q&A

Product Details

Alternative Names

E-Tag

Raised in

Mouse

Species Reactivity

N/A

Immunogen

GAPVPYPDPLEPRC synthetic peptide conjugate to KLH

Conjugate

Non-conjugated

Clonality

Monoclonal Antibody

Isotype

IgG1

Clone No.

11H12B3

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IP

Recommended Dilution

Application	Recommended Dilution
ELISA	1:4000-1:8000
WB	1:1000-1:320000
IP	1μg-5μg

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

E-Tag is a widely utilized peptide fusion tag for recombinant protein expression, detection, purification, and identification in molecular cloning and protein engineering workflows. CSB-MA000151M0m is a mouse monoclonal IgG1 antibody generated against KLH-conjugated GAPVPYPDPLEPRC synthetic peptide, purified via Protein G chromatography with over 95% purity to deliver consistent lot-to-lot performance and ultra-low non-specific cross-reactivity against untagged proteins. As a highly specific tag antibody, it exclusively recognizes E-tagged fusion constructs with no detectable signal on tag-free controls. ELISA exhibits strong dose-dependent binding curves with minimal background, Western blot clearly detects as low as 3.125 ng E-tagged protein and maintains distinct bands at dilutions up to 1:320000 across multiple fusion molecular weights, while IP efficiently pulls down E-tagged target protein from transfected 293F cell lysate with no enrichment in isotype control lanes. Validated for ELISA, WB, and IP, this antibody offers broad, flexible dilution ranges to support E-Tagged recombinant protein screening, quantification, and pull-down experiments in protein research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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