E-Tag Monoclonal Antibody

Datasheet

Code	CSB-MA000151M0m
Size
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Image	Western Blot Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: E-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Western Blot Positive WB detected in: E-tagged fusion protein All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Immunoprecipitating E-Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg) Lane 3: 293F transfected whole cell lysate (20µg) ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag) Antigen at dilution of 1µg/ml E-Tag antibody at (0-0.15µg) Goat polyclonal to Mouse IgG at 1/50000 dilution Western Blot Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag All lanes: E-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 30, 68, 55, 67-157kDa Observed band size: 30, 68, 55, 67-157kDa
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Product Details
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Product Details

Target Names

E-Tag

Alternative Names

E-Tag

Raised in

Mouse

Species Reactivity

All

Immunogen

GAPVPYPDPLEPRC synthetic peptide conjugate to KLH

Conjugate

Non-conjugated

Clonality

Monoclonal Antibody

Isotype

IgG1

Clone No.

11H12B3

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IP

Recommended Dilution

Application	Recommended Dilution
ELISA	1:4000-1:8000
WB	1:1000-1:320000
IP	1μg-5μg

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The monoclonal anti-E-Tag (IgG1 isotype) antibody is produced from the hybridomas fused by the myeloma cells and mouse splenocytes. The splenocytes are isolated from the mouse immunized with the E-tag synthetic peptide conjugated to KLH. This unconjugated E-Tag monoclonal antibody is purified through protein G with a purity of more than 95%. It can react with all E-Tag-fused proteins. And it is amenable to ELISA, WB, and IP applications.

E-Tag is a short amino acid sequence (GAPVPYPDPLEPR) that is frequently used as an epitope tag in molecular biology experiments to facilitate the detection and purification of recombinant proteins. The E-Tag sequence is genetically fused to the protein of interest, and antibodies or affinity resins that specifically recognize the E-Tag can be used to detect or purify the tagged protein. This allows researchers to study the expression, localization, and function of the protein in cells or tissues.

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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