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| Code | CSB-MA000151M0m |
| Size | |
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| Application | Recommended Dilution |
|---|---|
| ELISA | 1:4000-1:8000 |
| WB | 1:1000-1:320000 |
| IP | 1μg-5μg |
The monoclonal anti-E-Tag (IgG1 isotype) antibody is produced from the hybridomas fused by the myeloma cells and mouse splenocytes. The splenocytes are isolated from the mouse immunized with the E-tag synthetic peptide conjugated to KLH. This unconjugated E-Tag monoclonal antibody is purified through protein G with a purity of more than 95%. It can react with all E-Tag-fused proteins. And it is amenable to ELISA, WB, and IP applications.
E-Tag is a short amino acid sequence (GAPVPYPDPLEPR) that is frequently used as an epitope tag in molecular biology experiments to facilitate the detection and purification of recombinant proteins. The E-Tag sequence is genetically fused to the protein of interest, and antibodies or affinity resins that specifically recognize the E-Tag can be used to detect or purify the tagged protein. This allows researchers to study the expression, localization, and function of the protein in cells or tissues.
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