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The monoclonal anti-E-Tag (mouse IgG1 isotype) is produced from the hybridoma fused by the mouse myeloma cells and splenocytes from the immunized mouse. The splenocytes are isolated from the mouse immunized with the E-tag synthetic peptide conjugated to KLH and can secret the E-Tag antibody. This unconjugated E-Tag antibody is purified through protein G with a purity of more than 95%. It only reacts with samples containing E-Tag. And it has been amenable for ELISA, WB, and IP applications.
The E-tag is a popular epitope tag containing 13 amino acids. It is commonly expressed or detected with the protein of interest as an N- or C-terminal fusion. Anti-E tag antibodies are amenable for the detection, isolation, and purification of E-tagged proteins.
|Immunogen||GAPVPYPDPLEPRC synthetic peptide conjugate to KLH|
|Purification Method||>95%, Protein G purified|
|Concentration||It differs from different batches. Please contact us to confirm it.|
|Buffer||Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
|Tested Applications||ELISA, WB, IP|
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
|Troubleshooting and FAQs||Antibody FAQs|
|Storage||Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.|
|Lead Time||Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.|
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