HA-Tag Monoclonal Antibody

Code CSB-MA000158
Size US$100
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  • IP antibody use: 5µg HA Mouse IgG1 per ml Lysate, WB 1:5000
    Lane 1: untransfected 293 cell lysate
    Lane 2: transfected 293 cell lysate with HA-tag fusion protein
    Lane 3: IP (untransfted 293 + anti-HA mAb + Protein G agarose)
    Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
    Lane 5: IP (transfected 293 + anti-HA mAb + Protein G agarose)
    Lane 6: IP (transfected 293 + Protein G)
    Lane 7: Recombinant protein (E.coli)

  • IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA)

  • 0.5µg HA fusion protein + Primary antibody dilution at 1:10000

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Product Details

Target Names
HA-Tag
Raised in
Mouse
Species Reactivity
N/A
Immunogen
Synthetic Peptide
Conjugate
Non-conjugated
Isotype
IgG
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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