HA-Tag Monoclonal Antibody

Code CSB-MA000299
Size US$119
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  • Western blot analysis of His-MBP-HA recombinant protein, diluted at 1:10000.
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Product Details

Target Names
HA-Tag
Raised in
Mouse
Species Reactivity
N/A
Immunogen
Synthetic Peptide
Immunogen Species
N/A
Conjugate
Non-conjugated
Isotype
IgG
Clone No.
23H1
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Form
Liquid
Tested Applications
ELISA,WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The HA-Tag monoclonal antibody (CSB-MA000299, mouse IgG isotype) is specifically developed for the detection of recombinant proteins fused with the HA (Hemagglutinin) epitope tag. Originating from the influenza virus hemagglutinin protein, the HA tag consists of a highly conserved 9-amino acid sequence (YPYDVPDYA). Its compact nature ensures minimal steric hindrance, allowing it to be fused to either the N- or C-terminus of target proteins without significantly affecting their native conformation or biological function.

Produced via hybridoma technology using a synthetic peptide as the immunogen, this antibody guarantees exceptional specificity and batch-to-batch consistency. Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity and robust immunoreactivity. As demonstrated in the Western Blot analysis, the antibody successfully detects the His-MBP-HA recombinant protein even at a high working dilution of 1:10,000. The distinct, crisp band observed on the membrane underscores the antibody's high titer, excellent affinity, and superior signal-to-noise ratio, making it a highly cost-effective reagent for routine laboratory use.

This antibody is an indispensable tool for molecular biologists. It enables reliable confirmation of successful protein expression, accurate assessment of protein molecular weight, and serves as a critical reagent for downstream purification and detection workflows.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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