| Code | CSB-MA000299 |
| Size | US$119 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
The HA-Tag monoclonal antibody (CSB-MA000299, mouse IgG isotype) is specifically developed for the detection of recombinant proteins fused with the HA (Hemagglutinin) epitope tag. Originating from the influenza virus hemagglutinin protein, the HA tag consists of a highly conserved 9-amino acid sequence (YPYDVPDYA). Its compact nature ensures minimal steric hindrance, allowing it to be fused to either the N- or C-terminus of target proteins without significantly affecting their native conformation or biological function.
Produced via hybridoma technology using a synthetic peptide as the immunogen, this antibody guarantees exceptional specificity and batch-to-batch consistency. Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity and robust immunoreactivity. As demonstrated in the Western Blot analysis, the antibody successfully detects the His-MBP-HA recombinant protein even at a high working dilution of 1:10,000. The distinct, crisp band observed on the membrane underscores the antibody's high titer, excellent affinity, and superior signal-to-noise ratio, making it a highly cost-effective reagent for routine laboratory use.
This antibody is an indispensable tool for molecular biologists. It enables reliable confirmation of successful protein expression, accurate assessment of protein molecular weight, and serves as a critical reagent for downstream purification and detection workflows.
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