His-Tag Monoclonal Antibody

The His-Tag stands as one of the most universally adopted affinity and epitope tags in modern molecular biology. Its widespread utility stems from the strong, specific coordination between the imidazole side chains of histidine and immobilized transition metal ions (such as Ni²⁺ or Co²⁺) under mildly denaturing conditions. Beyond its dominant role in rapid one-step affinity purification, the His-tag serves as an exceptionally reliable handle for the immunological detection of recombinant proteins across diverse host systems, including bacteria, yeast, insect, and mammalian cells.

The Mouse Anti-His-Tag Monoclonal Antibody (CSB-MA000288, IgG isotype) is meticulously developed to provide high-affinity, monospecific recognition of this unique peptide sequence. Produced via robust hybridoma technology using a synthetic peptide as the immunogen, this antibody guarantees exceptional specificity, lot-to-lot consistency, and minimal cross-reactivity with endogenous cellular components.

Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity and a high binding affinity. The accompanying Western Blot analysis, conducted at a high working dilution of 1:5,000, demonstrates its remarkable immunoreactivity. In Lane 1, the antibody successfully detects the His-Tag in transiently transfected 293T mammalian cells, confirming its efficacy in eukaryotic expression systems. Similarly, Lane 2 reveals a crisp, distinct band for the purified His-MBP (Maltose-Binding Protein) recombinant protein. The clear visualization of both signals, even at this high dilution, underscores the antibody's excellent signal-to-noise ratio and cost-effectiveness for routine laboratory use.

This antibody is an indispensable tool for protein engineers and molecular biologists. It enables reliable confirmation of successful protein expression, accurate assessment of protein molecular weight, and serves as a critical reagent for evaluating the efficiency of affinity purification workflows. With its proven performance across prokaryotic and eukaryotic platforms, the His-Tag Monoclonal Antibody (CSB-MA000288) ensures dependable, high-quality results to support cutting-edge research in protein characterization, structural biology, and biopharmaceutical development.