KT3-Tag Monoclonal Antibody

Derived from the SV40 large T antigen, the KT3 tag is a compact 11-amino acid peptide sequence (KPPTPPPEPET). Frequently fused to the termini of target proteins, this epitope serves as a highly effective immunological handle for recombinant protein detection, affinity purification, and subcellular localization studies.

The Mouse Anti-KT3-Tag Monoclonal Antibody (CSB-MA000170, IgG isotype) is meticulously developed to provide researchers with monospecific and reliable recognition of this tag. Produced via robust hybridoma technology utilizing a synthetic peptide immunogen, this reagent ensures exceptional specificity and consistent lot-to-lot reproducibility.

Validated for both Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB) applications, this antibody exhibits outstanding sensitivity. As vividly demonstrated in the accompanying Western Blot analysis, the antibody successfully detects 1 µg of KT3 fusion protein at impressively high working dilutions of 1:5,000 and 1:10,000. The presence of distinct, intensely staining bands at both concentrations—conspicuously devoid of background interference or non-specific artifacts—unequivocally confirms the antibody's superior immunoreactivity and high signal-to-noise ratio. Such high titers translate into significant cost savings, making it a remarkably economical and powerful tool for demanding laboratory applications.

This monoclonal antibody is an indispensable asset for scientists engaged in molecular and cellular biology. It acts as a definitive reagent for verifying KT3-tagged protein expression, accurately assessing molecular weight, and monitoring the efficiency of affinity purification protocols. With its proven reliability, the KT3-Tag Monoclonal Antibody consistently delivers high-fidelity results, effectively supporting groundbreaking research in protein engineering and related disciplines.