MBP-Tag Monoclonal Antibody

Maltose-Binding Protein (MBP), derived from the Escherichia coli malE gene, is a highly soluble and stable protein widely utilized in molecular biology as a fusion tag to enhance the solubility and expression levels of recombinant target proteins. Beyond its role as an affinity tag for purification via maltose-based chromatography, MBP serves as an exceptional molecular chaperone, facilitating the correct folding of challenging hydrophobic or insoluble proteins.

The Mouse Anti-MBP-Tag Monoclonal Antibody (CSB-MA000163, IgG isotype) has been meticulously generated using highly purified recombinant MBP protein as the immunogen. Produced via robust hybridoma technology, this reagent guarantees exceptional monospecificity and consistent lot-to-lot reproducibility, ensuring reliable results across different experimental batches.

Validated for both Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB), this antibody exhibits remarkable sensitivity and high binding avidity. As vividly demonstrated in the accompanying Western Blot analysis, the antibody successfully detects 0.5 µg of MBP fusion protein at impressively high primary antibody working dilutions ranging from 1:3,000 to 1:10,000. The presence of distinct, intensely staining bands at all tested concentrations—completely devoid of background interference or non-specific artifacts—unequivocally confirms the antibody's superior immunoreactivity and excellent signal-to-noise ratio. Such potent performance at minimal concentrations not only ensures high fidelity in detection but also offers significant cost-efficiency for various laboratory applications.

This monoclonal antibody serves as an indispensable tool for scientists engaged in advanced protein engineering and molecular biology. It provides a definitive method for verifying MBP-tagged protein expression, accurately assessing molecular weight, and monitoring the efficiency of recombinant protein purification.