Myc tag Monoclonal Antibody

Datasheet
Code CSB-MA000041M0m
See More Details Free Antibody trial simple
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
    Lane 1: Recombinant Myc-tagged fusion protein at 50ng
    Lane 2: Recombinant Myc-tagged fusion protein at 25ng
    Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
    Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
    Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
    Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 50 kd
    Observed band size: 50 kd

  • Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating MYC-tag with transfected HEK293
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
    Lane 3: Transfected HEK293 whole cell lysate (10µg)

Product Details

Target Names Myc tag
Alternative Names bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
Raised in Mouse
Species Reactivity N/A
Immunogen EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Clone No. 5F92F9
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:100-1:300
IP 1:1000-1:1500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex. Abad M A, et al,The Journal of Cell Biology,2019

Sample type: HeLa Kyoto cells
Sample species: Mouse
Antibody dilution factor: 1:1000
Application: WB
Review: Analysis of the expression levels of the Myc-Borealin constructs used in Fig. 4 (B and C).
PMID: 31570499

Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Jeon Y.et al,Plant Mol Biol,2017

Application: Immunoblotting
Antibody dilution factor: 1:5000
Review: Since YFPN and YFPC are fused to Myc and HA tags, respectively, NbRbgA:YFPN and cpRPL:YFPC proteins were detected by immunoblotting using anti-Myc and anti-HA antibodies, respectively.
PMID: 29038916

Q&A and Customer Reviews

 Customer Reviews
  • Tested application: WB
    Test sample: Cells
    Sample volume: 4μg
    Electrophoresis parameters: 100V
    Primary antibody dilution ratio: 1:500
    Review: Antibody specificity is very good.
  • Tested application: WB
    Test sample: HEK293 cell lysate
    Sample volume: 20μg
    Electrophoresis parameters: 80V/100V
    Primary antibody dilution ratio: 1:1000
    Review: Myc antibody has good specificity.

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