Myc tag Monoclonal Antibody

Code CSB-MA000041M0m
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
    Lane 1: Recombinant Myc-tagged fusion protein at 50ng
    Lane 2: Recombinant Myc-tagged fusion protein at 25ng
    Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
    Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
    Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
    Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 50 kd
    Observed band size: 50 kd

  • Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating MYC-tag with transfected HEK293
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
    Lane 3: Transfected HEK293 whole cell lysate (10µg)

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Product Details

Description
Myc tag monoclonal antibody CSB-MA000041M0m was produced in the mouse immunized by using the EQKLISEEDL (Myc) synthetic peptide conjugate to KLH as the immunogen. The target peptide Myc is a popular short peptide tag (EQKLISEEDL) derived from the c-myc gene product and recognized by numerous commercial antibodies as a tag. This tag can be added to the C- or N-terminus by recombinant DNA technology and can be used for affinity chromatography and for isolating protein complexes with multiple subunits.
This Myc tag monoclonal antibody was tested in the ELISA, WB, IF and IP. The non-conjugated IgG1 got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of Myc tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of Myc fusion-expressed proteins and the like. It doesn’t have species restricted.
Target Names Myc tag
Alternative Names bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
Raised in Mouse
Species Reactivity N/A
Immunogen EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Clone No. 5F92F9
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:100-1:300
IP 1:1000-1:1500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 4 reviews

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Applications : Western Blot (WB)

Sample type: Cells

Sample dilution: 1:500

Review: Antibody specificity is very good.

By Anonymous

Applications : Western Blot (WB)

Sample type: HEK293 cell lysate

Sample dilution: 1:1000

Review: Myc antibody has good specificity.

By Anonymous

Applications : Immunoblotting

Sample dilution: 1:5000

Review: Since YFPN and YFPC are fused to Myc and HA tags, respectively, NbRbgA:YFPN and cpRPL:YFPC proteins were detected by immunoblotting using anti-Myc and anti-HA antibodies, respectively.

By Anonymous

Applications : WB

Sample type: Mouse HeLa Kyoto cells

Sample dilution: 1:1000

Review: Analysis of the expression levels of the Myc-Borealin constructs used in Fig. 4 (B and C).

By Anonymous

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