Myc tag Monoclonal Antibody

Datasheet
Code CSB-MA000041M0m
Product Type Monoclonal Antibody
Size US$200Purchase it in Cusabio online store
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Image
  • WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
    Lane 1: Recombinant Myc-tagged fusion protein at 50ng
    Lane 2: Recombinant Myc-tagged fusion protein at 25ng
    Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
    Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
    Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
    Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 50 kd
    Observed band size: 50 kd

  • Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating MYC-tag with transfected HEK293
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
    Lane 3: Transfected HEK293 whole cell lysate (10µg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Immunogen EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
Raised in Mouse
Tested Applications ELISA, WB, IF, IP; Recommended dilution: WB:1:500-1:5000, IF:1:100-1:300, IP:1:1000-1:1500
Relevance Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein. It can help to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Myc Tag also can be used to isolate protein complexes with multiple subunits. The Myc Tag Antibody is produced by the conjugation of a synthetic Myc tag peptide to KLH. Myc Tag Antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG1
Clonality Monoclonal
Alias bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 5F92F9
References
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Q&A and Customer Reviews

Customer Reviews
  • Tested application: WB
    Test sample: Cells
    Sample volume: 4μg
    Electrophoresis parameters: 100V
    Primary antibody dilution ratio: 1:500
    Review: Antibody specificity is very good.
  • Tested application: WB
    Test sample: HEK293 cell lysate
    Sample volume: 20μg
    Electrophoresis parameters: 80V/100V
    Primary antibody dilution ratio: 1:1000
    Review: Myc antibody has good specificity.

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