GST Monoclonal Antibody

Code CSB-MA000031M0m
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western blot
    All lanes : Recombinant GST Protein at 30ng/ml
    Line 1: Mouse Anti-GST monoclonal antibody at 1:1000
    Line 2: Mouse Anti-GST monoclonal antibody at 1:2000
    Line 3: Mouse Anti-GST monoclonal antibody at 1:5000
    Line 4: Mouse Anti-GST monoclonal antibody at 1:10000
    Line 5: Mouse Anti-GST monoclonal antibody at 1:20000
    Line 6: Mouse Anti-GST monoclonal antibody at 1:40000
    Line 7: Mouse Anti-GST monoclonal antibody at 1:80000
    Predicted band size : 28 kd
    Observed band size : 28 kd

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Product Details

Description
GST monoclonal antibody CSB-MA000031M0m was produced in the mouse immunized by using the Recombinant GST Protein as the immunogen. GST can be used to purify a protein by inserting its coding sequence next to your protein of interest. They will then be expressed as a fusion protein after transcription and translation. The GST tag system has the characteristics of high protein expression yield, convenient purification of expression products, and favorable preparation of GST antibodies.
This GST Monoclonal Antibody was tested in the WB and ELISA applications. The non-conjugated IgG2b got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of GST-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.
Target Names GST
Alternative Names Glutathione S-Transferase, gst, GST tag
Raised in mouse
Species Reactivity N/A
Immunogen Recombinant GST Protein
Conjugate Non-conjugated
Clonality monoclonal
Isotype IgG2b
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form liquid
Tested Applications ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Ubiquitin-Conjugating Enzyme E2 E Inhibits the Accumulation of Rice Stripe Virus in Laodelphax striatellus (Fallén). Y Li,Viruses,2020

Applications: GST Pull-Down Assay
Review: Proteins were then separated by SDS–PAGE and detected using anti-GST and anti-LstrE3 antibodies.
PMID: 32825037

The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission. Y Li,PLoS Pathog,2020

Applications: GST pull-down assay
Review: GST pull-down assays. LsTUB was fused to GST and incubated with viruliferous SBPH extracts or GST (control). Blots were probed with anti-NS3 or anti-GST antibodies.
PMID: 32817722

Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane. B Lee.et al,Biochimica et biophysica acta-molecular cell research,2017

Applications: WB
Antibody dilution factor: 1: 3000
Review: The in vitro translated emerin deletion mutants were subjected to GST pull-down assays with purified GST and GST-NICD. Bound proteins were analyzed by western blotting with anti-HA antibody. The purified proteins were subjected to western blot analysis with anti-GST antibody.
PMID: 27865926

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