GST Monoclonal Antibody

Datasheet
Code CSB-MA000031M0m
Product Type Monoclonal Antibody
Size US$200Purchase it in Cusabio online store
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Image
  • Western blot
    All lanes : Recombinant GST Protein at 30ng/ml
    Line 1: Mouse Anti-GST monoclonal antibody at 1:1000
    Line 2: Mouse Anti-GST monoclonal antibody at 1:2000
    Line 3: Mouse Anti-GST monoclonal antibody at 1:5000
    Line 4: Mouse Anti-GST monoclonal antibody at 1:10000
    Line 5: Mouse Anti-GST monoclonal antibody at 1:20000
    Line 6: Mouse Anti-GST monoclonal antibody at 1:40000
    Line 7: Mouse Anti-GST monoclonal antibody at 1:80000
    Predicted band size : 28 kd
    Observed band size : 28 kd

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen Recombinant GST Protein
Raised in mouse
Tested Applications ELISA, WB; Recommended dilution: WB:1:500-1:5000
Relevance Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Form liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG2b
Clonality monoclonal
Alias Glutathione S-Transferase, gst, GST tag
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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