Sumo tag Monoclonal Antibody

Code CSB-MA000132M0m
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
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  • WB:SUMO-tagged fusion protein(20ng/ml) was subjected to SDS-PAGE followed by Western Blot with CSB-MA000132M0m at dilution of
    Lane 1:1000 Lane 4:8000 Lane 7:64000
    Lane 2:2000 Lane 5:16000 Lane 8:128000
    Lane 3:4000 Lane 6:32000 Lane 9:256000
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size:55kd
    Observed band size:55kd

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Product Details

Description

The mice spleens isolated from sumo tag-immunized mice are fused with myeloma cells to generate hybridomas. Hybridoma cells are further injected into the abdominal cavity of mice, producing and obtaining mouse ascites fluid containing sumo tag monoclonal antibody. This monoclonal antibody occurs as an unconjugated IgG1. Its purity is greater than 95% using protein G purified. The target sumo tag is commonly used as an N-terminus fusion sequence in yeast to increase the expression and solubility of the wanted recombinant proteins. And this anti-sumo tag monoclonal antibody has been validated for use in the ELISA and WB assays.

Target Names Sumo tag
Alternative Names SUMO tag,SUMO
Raised in mouse
Species Reactivity N/A
Immunogen sumo tag protein
Conjugate Non-conjugated
Clonality monoclonal
Isotype IgG1
Purification Method >95%,Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form liquid
Tested Applications ELISA,WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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