S-Tag Monoclonal Antibody

Originating from the pancreatic ribonuclease A (RNase A), the S-Tag is a highly practical 15-amino-acid peptide sequence (KETAAAKFERQHMDS). Initially characterized by its remarkable ability to refold and restore enzymatic activity upon binding with its cognate S-protein, this specific high-affinity interaction has been ingeniously adapted for recombinant protein research. As a fusion tag, the S-Tag is exceptionally compact, ensuring minimal steric hindrance and negligible impact on the native folding or biological function of the target molecule.

The Mouse Anti-S-Tag Monoclonal Antibody (CSB-MA000172, IgG subclass) is expertly developed to provide precise, monospecific recognition of this linear epitope. Produced via rigorous hybridoma technology utilizing a synthetic peptide immunogen, this reagent guarantees exceptional specificity and robust lot-to-lot consistency across experimental batches.

Validated for both ELISA and Western Blot (WB) applications, this antibody exhibits extraordinary sensitivity and binding capacity. As vividly demonstrated in the accompanying Western Blot analysis, the antibody successfully identifies 2 µg of the S-Tag fusion protein at impressively high working dilutions of 1:5,000 (Lane 1) and 1:10,000 (Lane 2). The distinct, intense bands observed at both concentrations, conspicuously free of background interference, smearing, or non-specific artifacts, unequivocally attest to the antibody's superior immunoreactivity and high signal-to-noise ratio. Such high titers render it a remarkably cost-effective solution for demanding laboratory environments.

This monoclonal antibody serves as an indispensable tool for researchers engaged in molecular and cellular biology. It acts as a definitive reagent for verifying successful S-Tag fusion protein expression, accurately assessing molecular weight, and serving as a robust handle for affinity purification and downstream biochemical assays. With its proven performance and adaptability, the S-Tag Monoclonal Antibody ensures consistently high-quality, dependable results, effectively supporting robust research in protein engineering and structural genomics.