TUBA1A Monoclonal Antibody

Code CSB-MA754656A0m
Size US$120
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Image
  • Western Blot
    Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,
    All lanes: TUBA1A antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa
     

  • Western Blot
    Positive WB detected in: Rabbit heatrt tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue
    All lanes: TUBA1A antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa
     

  • Western Blot
    Positive WB detected in: Rat brain tissue, Rat stomach tissue, Rat kidney tissue
    All lanes: TUBA1A antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa
     

  • Western Blot
    Positive WB detected in: NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse brain tissue, Mouse kidney tissue
    All lanes: TUBA1A antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa
     

  • Western Blot
    Positive WB detected in: Hela whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg
    All lanes: TUBA1A antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa
     

  • Western Blot
    Positive WB detected in: Hela whole cell lysate
    All lanes: TUBA1A antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa
     

  • Western Blot
    Positive WB detected in: Hela whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg, 0.3125µg, 0.15625µg
    All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa
     

  • Western Blot
    Positive WB detected in: Hela whole cell lysate
    All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 320000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa
     

  • IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-MA754656A0m at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating TUBA1A in Hela whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA754656A0m (5µg) + Hela whole cell lysate (500µg)
    Lane 3: Hela whole cell lysate (20µg)
     

  • Immunoprecipitating TUBA1A in Hela whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: Company A (5µg), Company B (5µg), Company C (5µg), Company D (5µg), CSB-MA754656A0m (5µg) + Hela whole cell lysate (500µg)
    Lane 3: Hela whole cell lysate (20µg)
     

  • Overlay histogram showing Hela cells stained with CSB-MA754656A0m (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • Overlay histogram showing Hela cells stained with Company A (5µg), Company B (5µg), Company C (5µg), Company D (5µg), CSB-MA754656A0m (5µg) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

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Product Details

Full Product Name
Mouse anti-Homo sapiens (Human) TUBA1A Monoclonal Antibody antibody
Uniprot No.
Target Names
TUBA1A
Alternative Names
Alpha tubulin 3 antibody; Alpha-tubulin 3 antibody; B alpha 1 antibody; FLJ25113 antibody; LIS3 antibody; TBA1A_HUMAN antibody; TUBA1A antibody; TUBA3 antibody; Tubulin alpha 1a antibody; Tubulin alpha 1A chain antibody; Tubulin alpha 3 antibody; Tubulin alpha 3 chain antibody; Tubulin alpha brain specific antibody; Tubulin alpha-1A chain antibody; Tubulin alpha-3 chain antibody; Tubulin B alpha 1 antibody; Tubulin B-alpha-1 antibody
Raised in
Mouse
Species Reactivity
Human, Rabbit, Rat, Mouse
Immunogen
A synthesized peptide derived from human Tubulin alpha-1A chain (297-309aa)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal Antibody
Isotype
IgG2a
Clone No.
7E5C12
Purification Method
>95%, Protein A purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF, FC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:20000-1:320000
IHC 1:100-1:300
IF 1:50-1:200
FC 1:100-1:300
IP 1µg-5µg
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The product CSB-MA754656A0m is an unconjugated monoclonal antibody against the human TUBA1A. It is produced from the mouse splenocyte-myeloma cell fused hybridoma. The splenocyte is isolated from the mouse immunized with the synthesized peptide that is derived from human TUBA1A protein (297-309aa). This TUBA1A monoclonal antibody can react with TUBA1A protein from four species, including human, rabbit, rat, and mouse. Protein A-mediated purification of this TUBA1A monoclonal antibody makes its purity up to more than 95%. This antibody has been validated for use in ELISA, WB, IHC, IF, IP, and FC applications.

TUBA1A protein plays a crucial role in the organization and stabilization of microtubules, which are important for maintaining the shape and structural integrity of cells. Additionally, TUBA1A has been shown to play a role in neuronal development and function, as it is a major constituent of the microtubules that form the axon and dendrites of neurons. Mutations in the TUBA1A gene are associated with a range of neurological disorders, including microcephaly, lissencephaly, and intellectual disability.

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Target Background

Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Gene References into Functions
  1. A de novo heterozygous c.320A>G [p.(His 107 Arg)] mutation in TUBA1A was identified in a patient with microcephaly, epileptic seizures, and severe developmental delay. PMID: 29109381
  2. Given that Spastin engages the MT in two places, we propose that severing occurs by forces exerted on the C-terminal tail of tubulin, which results in a conformational change in tubulin, which releases it from the polymer. PMID: 17389232
  3. Molecular docking studies revealed that 6f interacted and bound ef fi ciently with the colchicine-binding site of tubulin. In addition, 6f treatment induced G2/M cell cycle arrest dose-dependently and subsequently induced cell apoptosis PMID: 28440465
  4. induced pluripotent stem cells (iPSCs) from the umbilical cord and peripheral blood of two lissencephaly patients with different clinical severities carrying alpha tubulin (TUBA1A) missense mutations, were generated. PMID: 27431206
  5. Long intergenic non-coding RNA APOC1P1-3 inhibits apoptosis by decreasing alpha-tubulin acetylation in breast cancer. PMID: 27228351
  6. Results show that Tuba1a plays an essential, noncompensated role in neuronal saltatory migration in vivo and highlight the importance of microtubule flexibility in nucleus-centrosome coupling and neuronal-branching regulation during neuronal migration. PMID: 28687665
  7. data suggest that the TUBA1A mutations disrupting lateral interactions have pronounced dominant-negative effects on microtubule dynamics that are associated with the severe end of the lissencephaly spectrum PMID: 26493046
  8. Data show that tubulin phosphorylation and acetylation play important roles in the control of microtubule assembly and stability. PMID: 26165356
  9. Data show that plasma membrane Ca(2+)-ATPase (PMCA) was associated with tubulin in normotensive and hypertensive erythrocytes. PMID: 26307527
  10. Studies indicate that alpha-tubulin acetylation and microtubule level is mainly governed by opposing actions of alpha-tubulin acetyltransferase 1 (ATAT1) and histone deacetylase 6 (HDAC6). PMID: 26227334
  11. Data from studies using peptide fragment of alpha-tubulin (residues 31-49) suggest that Ser38 is crucial for substrate recognition by alpha-tubulin acetylase 1 (ATAT1); Asp39, Ile42, the glycine stretch (residues 43-45), and Asp46 are also involved. PMID: 25602620
  12. Lysine 40 acetylation of alpha-tubulin does not result in significant changes in kinesin-1's landing rate or motility parameters. PMID: 24940781
  13. These results demonstrated that SelP interacts with tubulin, alpha 1a (TUBA1A). PMID: 24914767
  14. This study show all foetuses with lissencephaly and cerebellar hypoplasia carried distinct TUBA1A mutations. PMID: 25059107
  15. These findings call attention to PKC-mediated phosphorylation of alpha-tubulin as a novel mechanism for controlling the dynamics of microtubules that result in cell movement. PMID: 24574051
  16. case provides new insight into the wide spectrum of disease phenotypes associated with TUBA1A mutation PMID: 23528852
  17. The present study confirms that mutations in tubulin genes are responsible for complex brain malformation. PMID: 24392928
  18. Studies suggest that tubulin-interactive agents have the potential to play a significant role in the fight against cancer. PMID: 23818224
  19. Missense mutations in TUBA1A were found in 3 patients with polymicrogyria. PMID: 22948023
  20. We described the clinical course and pathological findings in a child with TUBA1A mutation PMID: 22633752
  21. TUBA1A and TUBB2B coding regions have been sequenced that are associated with cortical malformations. PMID: 23361065
  22. Data show that Na(+),K(+)-ATPase activity was >50% lower and membrane-associated tubulin content was >200% higher in erythrocyte membranes from diabetic patients. PMID: 22565168
  23. study describes a 14-month-old girl with TUBA1A mutation-associated lissencephaly, and summarize the clinical and neuroradiologic findings of 19 cases in the literature PMID: 22264709
  24. Alpha2B-adrenergic receptor interaction with tubulin controls its transport from the endoplasmic reticulum to the cell surface PMID: 21357695
  25. The expression of alpha-tubulin and MDR1 may play an important role in the development and progression of human non-small cell lung carcinoma. PMID: 20510079
  26. We report a mutation in TUBA1A as a cause of polymicrogyria. So far, all mutations in TUBA1A have occurred de novo, resulting in isolated cases. This article describes familial recurrence of TUBA1A mutations due to somatic mosaicism in a parent. PMID: 21403111
  27. Data show that IAV-infected cells contain elevated level of AcTub and alpha-tubulin. PMID: 21094644
  28. Mutations in TUBA1A result in defects in the tubulin folding and heterodimer assembly. PMID: 20603323
  29. LIS-associated mutations of TUBA1A operate via diverse mechanisms that include disruption of binding sites for microtubule-associated proteins. PMID: 20466733
  30. the dipole moments of each tubulin isotype may influence their functional characteristics within the cell, resulting in differences for MT assembly kinetics and stability PMID: 16941085
  31. Mutations in alpha-tubulin in mice and humans that affect neuronal migration result in abnormal lamination of brain structures with associated behavioral deficits. PMID: 17218254
  32. Retrospective examination of MR images suggests that patients with TUBA1A mutations share not only cortical dysgenesis, but also cerebellar, hippocampal, corpus callosum, and brainstem abnormalities PMID: 17584854
  33. Increased expression of tubulin alpha is associated with pulmonary sclerosing hemangioma PMID: 17914564
  34. The diminished production of TUBA1A tubulin in R264C individuals is consistent with haploinsufficiency as a cause of the disease phenotype. PMID: 18199681
  35. the TUBA1A phenotype is distinct from LIS1, DCX, RELN and ARX lissencephalies. Compared with the phenotypes of children mutated for TUBA1A, these prenatally diagnosed fetal cases occur at the severe end of the TUBA1A lissencephaly spectrum. PMID: 18669490
  36. Missense mutations within the TUBA1A gene are associated with specific abnormalities in lissencephaly. PMID: 18728072
  37. mutation analysis in the TUBA1A gene in 46 patients with classical lissencephaly. PMID: 18954413
  38. This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953

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Involvement in disease
Lissencephaly 3 (LIS3)
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Tubulin family
Tissue Specificity
Expressed at a high level in fetal brain.
Database Links

HGNC: 20766

OMIM: 602529

KEGG: hsa:7846

STRING: 9606.ENSP00000301071

UniGene: Hs.654422

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