V5-Tag Monoclonal Antibody

Code CSB-MA000160
Size US$167
  • IP antibody use: 5µg V5 Mouse IgG1 per ml Lysate, WB 1: 5000
    Lane 1: untransfected 293 cell lysate
    Lane 2: transfected 293 cell lysate with V5-tag fusion protein
    Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose)
    Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
    Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose)

  • IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)

  • 1µg V5 fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

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Product Details

V5-Tag monoclonal antibody CSB-MA000160 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The V5 tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
This V5-Tag Monoclonal Antibody was tested in the ELISA, WB, IF and IHC applications. V5-Tag is one of the epitope tags, which provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
Target Names V5-Tag
Raised in Mouse
Species Reactivity N/A
Immunogen Synthetic Peptide
Conjugate Non-conjugated
Isotype IgG
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form Liquid
Tested Applications ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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