IP antibody use: 5µg V5 Mouse IgG1 per ml Lysate, WB 1: 5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with V5-tag fusion protein
Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose)
IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)
1µg V5 fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.