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V5-Tag Monoclonal Antibody

Datasheet
Code CSB-MA000160
Size US$100
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  • CSB-MA000160-IP-1

    IP antibody use: 5µg V5 Mouse IgG1 per ml Lysate, WB 1: 5000
    Lane 1: untransfected 293 cell lysate
    Lane 2: transfected 293 cell lysate with V5-tag fusion protein
    Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose)
    Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
    Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose)

  • CSB-MA000160-IF-1

    IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)

  • CSB-MA000160-WB-1

    1µg V5 fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

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Product Details

Target Names
V5-Tag
Raised in
Mouse
Species Reactivity
N/A
Immunogen
Synthetic Peptide
Conjugate
Non-conjugated
Isotype
IgG
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
IP 1:200-1:2000
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
V5-Tag monoclonal antibody CSB-MA000160 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The V5 tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
This V5-Tag Monoclonal Antibody was tested in the ELISA, WB, IF and IHC applications. V5-Tag is one of the epitope tags, which provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.

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