VSV-G-Tag Monoclonal Antibody

The VSV-G tag, derived from the C-terminal region of the Vesicular Stomatitis Virus (VSV) envelope glycoprotein, has emerged as an exceptionally versatile and powerful tool in modern molecular and cell biology. Renowned for its extremely strong affinity to liposomes and its ability to facilitate efficient pH-dependent membrane fusion, this 11-amino acid peptide sequence (YTDIEMNRLGK) is ubiquitously employed for the production and concentration of viral vectors, such as lentivirus and retrovirus, as well as for enhancing the solubility of challenging recombinant fusion proteins.

The Mouse Anti-VSV-G-Tag Monoclonal Antibody (CSB-MA000161, IgG isotype) has been meticulously developed to provide researchers with a reagent of the highest purity and monospecificity. Generated through rigorous hybridoma technology utilizing a precisely synthesized peptide immunogen, this antibody was subsequently subjected to stringent affinity chromatography using the specific immunogen to purify it from mouse ascites. This sophisticated multi-step purification process guarantees exceptional batch-to-batch consistency and eliminates non-specific background noise.

Validated across an extensive spectrum of applications including Enzyme-Linked Immunosorbent Assay (ELISA), Western Blotting (WB), Immunofluorescence (IF), and Immunoprecipitation (IP), this antibody exhibits remarkable versatility and robust binding avidity. As vividly demonstrated in the accompanying multi-platform validation data, the antibody successfully detects VSV-G fusion proteins with high sensitivity and precision. The Western Blot panels reveal distinct, sharply defined bands at various working dilutions, completely devoid of background interference. Furthermore, its efficacy in IP and IF applications underscores its capability to reliably capture and visualize native protein complexes within complex cellular environments.

This monoclonal antibody stands as an indispensable asset for scientists engaged in advanced virology, gene therapy, and protein engineering. It provides a definitive method for verifying VSV-G-tagged protein expression, accurately assessing molecular weight, and meticulously monitoring the efficiency of recombinant protein purification or viral particle assembly. With its proven reliability across multiple experimental paradigms, the VSV-G-Tag Monoclonal Antibody consistently delivers high-fidelity results, effectively supporting cutting-edge research and development in vector production and beyond.