VSV-G-Tag Monoclonal Antibody

IP antibody use: 5µg VSV-G Mouse IgG1 per ml Lysate, WB 1:5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with VSV-G-tag fusion protein
Lane 3: IP (untransfted 293 + anti-VSV-G mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-VSV-G mAb + Protein G agarose)
Lane 6: IP (transfected 293 + Protein G)
Lane 7: Recombinant protein (E.coli)

IF analysis of 293T cells transfected with a VSV-G-tagged protein,1:2000 dilution (blue DAPI, red anti-VSV-G)

1µg VSV-G fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000