Recombinant Chicken Occludin (OCLN)

Code CSB-CF016263CH
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
OCLN
Uniprot No.
Alternative Names
OCLN; Occludin
Species
Gallus gallus (Chicken)
Expression Region
1-504
Target Protein Sequence
MFSKKSYDGPPAGYGPPTGYGAPTADYGYGSPPPGSYYVDDAPQLFYKWTSPPGAVRGLQ AGVLVLCIAIFACVASTLAWDYGYGLGGAYGTGLGGFYGSNYYGSGLSYSYGYGGYYGGV NQRTANGFMIAMAVLCFLAQLGLLVAALSKSGATRSRRFYLAVLVLSAVLAFVMLIASIV YIMGVNPQAQMSSGYYYSPLLAMCSQAYGSTYLNQYIYHYCTVDPQEAVAAVCGFLIVIL LCLICFFAQKTRSKIWRYGKANIYWDRAPVVQEGPDVEEWVKNVADGASVQDETATLAYS EKPTSPVAAPPYSYVPPPSAGYYPSGTYSSRGDQPDRALSASPVHGEEEEEKGKDQPSRP PARRGRRRRRNPELDESQYETDYTTAVESSDERDQEQWASLYPPITSDGARQRYKQEFDT DLKRYKQLCAEMDSINDRLNQLSRRLDSITEDSPQYQDVAEEYNQLKDLKRSPDYQSKKQ ESKVLRNKLFHIKRMVSAYDKVRG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Interacts with ZO-1.
Gene References into Functions
  1. the level of feed intake is related to or may directly regulate OCLN mRNA expression or may have an indirect effect through paracrine or autocrine factors in the ovary. PMID: 28238709
  2. Dietary Zn supplementation appeared to alleviate the loss of intestinal mucosal barrier function induced by S. Typhimurium challenge and the partial mechanism might be related to the increased expression of occludin and claudin-1 in broiler chickens. PMID: 22834550
  3. a unique motif in the occludin sequence that is involved in the regulation of ZO-1 binding by reversible phosphorylation of specific Tyr residues. PMID: 19017651
Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, tight junction.
Protein Families
ELL/occludin family
Tissue Specificity
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in lung and liver. Expressed at a lower level in brain.
Database Links

KEGG: gga:396026

UniGene: Gga.603

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