Recombinant Chicken Tyrosinase (TYR)

Code CSB-CF025394CH
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
TYR
Uniprot No.
Alternative Names
TYR; Tyrosinase; Monophenol monooxygenase
Species
Gallus gallus (Chicken)
Expression Region
19-529
Target Protein Sequence
QFPRVCANTQSLLRKECCPPWDGDGTPCGERSNRGTCQRILLSQAPLGPQFPFSGVDDREDWPSVFYNRTCRCRGNFMGFNCGECKFGFSGQNCTERRLRTRRNIFQLTISEKDKFLAYLNLAKNIPSKDYVIATGTYAQMNNGSNPMFRNINVYDLFVWMHYYASRDTLLGGSNVWRDIDFAHEAPGFLPWHRAFLLLWEREIQKITGDENFTIPYWDWRDAEDCVICTDEYMGGQHPTNPNLLSPASFFSSWQVICTQSEEYNSQQALCNATSEGPILRNPGNNDKSRTPRLPSSSEVEFCLTLTQYESGSMDKMANYSFRNTLEGFADPHTAISNISQSGLHNALHIYMNGSMSQVQGSANDPIFILHHAFVDSIFERWLRRHRPMLEVYPAANAPIGHNRENYMVPFIPLYRNGEFFISSRELGYDYEYLQEPALGSFQDFLIPYLKQAHQIWPWLVGAAVIGGIITAVLSGLILACRKKRKGTSPEIQPLLTESEDYNNVSYQSHF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
Gene References into Functions
  1. Individuals with genotypes AA and AT had greater TYR expression than those with genotype TT. A luciferase assay of the promoter activity revealed that genotype AA had greater activity than genotype TT. PMID: 29050424
  2. The co-segregation of plumage coloration and sequence polymorphism in TYR and MC1R genes were investigated using an intercross between black and white plumage color types of the Dongxiang blue-shelled chicken. PMID: 20460655
  3. This study provided original information for elucidating the possible roles of exon-1 of TYR gene and 5' flanking region in chickens with different flesh color chicken. PMID: 19437131
  4. Mutated proteins showed the insertion of a complete avian retroviral sequence of the Avian Leukosis Virus (ALV) family. PMID: 16457736
  5. An avian retroviral sequence insertion in the tyrosinase gene was the cause of recessive white phenotype in chickens. PMID: 17878441

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Subcellular Location
Melanosome membrane; Single-pass type I membrane protein. Melanosome.
Protein Families
Tyrosinase family
Database Links
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